اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها2,998
تعداد مقالات33,579
تعداد مشاهده مقاله44,251,577
تعداد دریافت فایل اصل مقاله20,622,651
Saberi, Hassan, Mehranfard, Nasrin, rezazadeh, Hossein, Ghasemi, Maedeh. (1403). Involvement of γ-Aminobutyric acid and N-methyl-D-aspartate receptors in diabetic gastropathy in rats: possible beneficial effect of prolonged treatment with insulin and magnesium supplement. سامانه مدیریت نشریات علمی, 79(3), 601-608. doi: 10.32592/ARI.2024.79.3.601
Hassan Saberi; Nasrin Mehranfard; Hossein rezazadeh; Maedeh Ghasemi. "Involvement of γ-Aminobutyric acid and N-methyl-D-aspartate receptors in diabetic gastropathy in rats: possible beneficial effect of prolonged treatment with insulin and magnesium supplement". سامانه مدیریت نشریات علمی, 79, 3, 1403, 601-608. doi: 10.32592/ARI.2024.79.3.601
Saberi, Hassan, Mehranfard, Nasrin, rezazadeh, Hossein, Ghasemi, Maedeh. (1403). 'Involvement of γ-Aminobutyric acid and N-methyl-D-aspartate receptors in diabetic gastropathy in rats: possible beneficial effect of prolonged treatment with insulin and magnesium supplement', سامانه مدیریت نشریات علمی, 79(3), pp. 601-608. doi: 10.32592/ARI.2024.79.3.601
Saberi, Hassan, Mehranfard, Nasrin, rezazadeh, Hossein, Ghasemi, Maedeh. Involvement of γ-Aminobutyric acid and N-methyl-D-aspartate receptors in diabetic gastropathy in rats: possible beneficial effect of prolonged treatment with insulin and magnesium supplement. سامانه مدیریت نشریات علمی, 1403; 79(3): 601-608. doi: 10.32592/ARI.2024.79.3.601

Involvement of γ-Aminobutyric acid and N-methyl-D-aspartate receptors in diabetic gastropathy in rats: possible beneficial effect of prolonged treatment with insulin and magnesium supplement

Archives of Razi Institute
مقاله 20، دوره 79، شماره 3، مرداد و شهریور 2024، صفحه 601-608    اصل مقاله (507.35 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.3.601
نویسندگان
Hassan Saberi1؛ Nasrin Mehranfard2؛ Hossein rezazadeh3؛ Maedeh Ghasemi* 3
1School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2Neurophysiology Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran
3Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده
Gastrointestinal dysfunction is a severe and common complication in diabetic patients. Some evidence shows that gamma-aminobutyric acid (GABA) and glutamate contribute to diabetic gastrointestinal abnormalities. Hence, we examined the impact of prolonged treatment with insulin and magnesium supplement on the expression pattern of GABA type A (GABA-A), GABA-B and N-methyl-D-aspartate (NMDA) glutamate receptors as well as nitric oxide synthase 1 (NOS-1) in the stomach of type 2 diabetic rats. Twenty-four male Wistar rats were randomized to 4 groups (6 rats each): 1) control, 2) type 2 diabetes: rats fed with a high fat diet for 3 months + low dose of streptozotocin (35 mg/kg), 3) type 2 diabetes + magnesium, and 4) type 2 diabetes + insulin. The expression of NOS-1 as well as GABA-A, GABA-B, and NMDA receptors was detected using western blotting. NOS-1 expression was substantially diminished (p<0.01) while the expression of GABA-A (p<0.001), GABA-B (p<0.001), and NMDA (p<0.001) receptors was enhanced in the stomach of diabetic rats relative to control. Treatment with magnesium and insulin improved NOS-1 expression in diabetic rats, although this effect was greater in magnesium treatment alone. Magnesium also restored the expression of GABA-A and GABA-B receptors in diabetic rats to control values. Moreover, insulin treatment improved GABA-A receptor expression in diabetic rats (p<0.05). No considerable alterations were detected in NMDA receptor level in the treatment groups. The results suggest a significant role of magnesium and insulin in improving gastric motility and secretory disorders associated with diabetes through modifying the expression of GABAergic receptors.
کلیدواژه‌ها
Type 2 Diabetes؛ Magnesium Supplement؛ GABA Receptor؛ NMDA Receptor؛ NOS-1
عنوان مقاله [English]
دخالت رسپتورهای گاما-آمینوبوتیریک اسید و ان-متیل-دی-آسپارتات در گاستروپاتی دیابتی در موش صحرائی: اثرات سودمند درمان بلند مدت انسولین و مکمل منیزیم
سایر فایل های مرتبط با مقاله
مراجع
  1. Meldgaard T, Brock C. Diabetes and the gastrointestinal tract. Medicine (Baltimore). 2019;47(7):454–9.
  2. Aleppo G, Calhoun P, Foster NC, Maahs DM, Shah VN, Miller KM. Reported gastroparesis in adults with type 1 diabetes (T1D) from the T1D Exchange clinic registry. J Diabetes Complications [Internet]. 2017
  3. Watkins CC, Sawa A, Jaffrey S, Blackshaw S, Barrow RK, Snyder SH, et al. Insulin restores neuronal nitric oxide synthase expression and function that is lost in diabetic gastropathy. J Clin Invest. 2000;106(3):373.
  4. Gangula PRR, Sekhar KR, Mukhopadhyay S. Gender bias in gastroparesis: is nitric oxide the answer? Dig Dis Sci [Internet]. 2011;56(9):2520–7.
  5. Hyland NP, Cryan JF. A Gut Feeling about GABA: Focus on GABA(B) Receptors. Front Pharmacol. 2010;1:124.
  6. Bettler B, Kaupmann K, Mosbacher J, Gassmann M. Molecular structure and physiological functions of GABA(B) receptors. Physiol Rev. 2004;84(3):835–67.
  7. Auteri M, Zizzo MG, Serio R. GABA and GABA receptors in the gastrointestinal tract: from motility to inflammation. Pharmacol Res. 2015;93:11–21.
  8. Lehmann A. GABAB receptors as drug targets to treat gastroesophageal reflux disease. Pharmacol Ther [Internet]. 2009;122(3):239–45.
  9. Piqueras L, Martinez V. Peripheral GABAB agonists stimulate gastric acid secretion in mice. Br J Pharmacol [Internet]. 2004;142(6):1038.
  10. Watanabe K, Kanno T, Oshima T, Miwa H, Tashiro C, Nishizaki T. Vagotomy upregulates expression of the N-methyl-D-aspartate receptor NR2D subunit in the stomach. J Gastroenterol. 2008;43(5):322–6.
  11. Baj A, Moro E, Bistoletti M, Orlandi V, Crema F, Giaroni C. Glutamatergic Signaling Along The Microbiota-Gut-Brain Axis. Int J Mol Sci [Internet]. 2019;20(6).
  12. Covasa M, Ritter RC, Burns GA. NMDA receptor participation in control of food intake by the stomach. Am J Physiol - Regul Integr Comp Physiol. 2000;278(5):R1362-8.
  13. Feng J, Wang H, Jing Z, Wang Y, Cheng Y, Wang W, et al. Role of Magnesium in Type 2 Diabetes Mellitus. Biol Trace Elem Res [Internet]. 2020;196(1):74–85. /
  14. Paolisso G, Sgambato S, Gambardella A, Pizza G, Tesauro P, Varricchio M, et al. Daily magnesium supplements improve glucose handling in elderly subjects. Am J Clin Nutr. 1992;55(6):1161–7.
  15. Solati M, Kazemi L, Shahabi Majd N, Keshavarz M, Pouladian N, Soltani N. Oral herbal supplement containing magnesium sulfate improve metabolic control and insulin resistance in non-diabetic overweight patients: A randomized double blind clinical trial. Med J Islam Repub Iran [Internet]. 2019;33:2.
  16. Rondón LJ, Privat AM, Daulhac L, Davin N, Mazur A, Fialip J, et al. Magnesium attenuates chronic hypersensitivity and spinal cord NMDA receptor phosphorylation in a rat model of diabetic neuropathic pain. J Physiol. 2010;588(Pt 21):4205.
  17. Choi KM, Gibbons SJ, Roeder JL, Lurken MS, Zhu J, Wouters MM, et al. Regulation of interstitial cells of Cajal in the mouse gastric body by neuronal nitric oxide. Neurogastroenterol Motil. 2007;19(7):585–95.
  18. Zizzo MG, Mulè F, Serio R. Functional evidence for GABA as modulator of the contractility of the longitudinal muscle in mouse duodenum: Role of GABAA and GABAC receptors. Neuropharmacology. 2007;52(8):1685-1690.
  19. Purwana I, Zheng J, Li X, Deurloo M, Son DO, Zhang Z, et al. GABA promotes human β-cell proliferation and modulates glucose homeostasis. Diabetes. 2014;63(12):4197–205.
  20. Tian J, Dang HN, Yong J, Chui WS, Dizon MPG, Yaw CKY, et al. Oral treatment with γ-aminobutyric acid improves glucose tolerance and insulin sensitivity by inhibiting inflammation in high fat diet-fed mice. PLoS One. 2011;6(9).
  21. Mehranfard N, Rezazadeh H, Soltani N, Dastgerdi AH, Ghanbari Rad M, Ghasemi M. Changes in Protease-Activated Receptor and Trypsin-1 Expression Are Involved in the Therapeutic Effect of Mg2+Supplementation in Type 2 Diabetes-Induced Gastric Injury in Male Adult Rats. Adv Pharmacol Pharm Sci. 2023:5703718.
  22. Mathew AA, Panonnummal R. ‘Magnesium’-the master cation-as a drug—possibilities and evidences. BioMetals. 2021;34(5):955–86.
  23. Hansen KB, Yi F, Perszyk RE, Menniti FS, Traynelis SF. NMDA receptors in the central nervous system. Methods Mol Biol. 2017;1677:1.
  24. Swaminathan M, Hill-Yardin EL, Bornstein JC, Foong JPP. Endogenous glutamate excites myenteric calbindin neurons by activating group I metabotropic glutamate receptors in the mouse colon. Front Neurosci. 2019;13:426.
  25. Gaggini M, Carli F, Rosso C, Buzzigoli E, Marietti M, Della Latta V, et al. Altered amino acid concentrations in NAFLD: Impact of obesity and insulin resistance. Hepatology. 2018;67(1):145–58.
  26. Liu X, Zheng Y, Guasch-Ferré M, Ruiz-Canela M, Toledo E, Clish C, et al. High plasma glutamate and low glutamine-to-glutamate ratio are associated with type 2 diabetes: Case-cohort study within the PREDIMED trial. Nutr Metab Cardiovasc Dis. 2019;29(10):1040.
  27. Wiegers EC, Rooijackers HM, van Asten JJA, Tack CJ, Heerschap A, de Galan BE, et al. Elevated brain glutamate levels in type 1 diabetes: correlations with glycaemic control and age of disease onset but not with hypoglycaemia awareness status. Diabetologia. 2019;62(6):1065.
  28. Marquard J, Otter S, Welters A, Stirban A, Fischer A, Eglinger J, et al. Characterization of pancreatic NMDA receptors as possible drug targets for diabetes treatment. Nat Med. 2015;21(4):363–72.
  29. Huang X-T, Yang J-X, Wang Z, Zhang C-Y, Luo Z-Q, Liu W, et al. Activation of N-methyl-D-aspartate receptor regulates insulin sensitivity and lipid metabolism. 2021;11(5):2247–62.
  30. Golovynska I, Beregova T V., Falalyeyeva TM, Stepanova LI, Golovynskyi S, Qu J, et al. Peripheral N-methyl-D-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies. Sci Reports. 2018;8(1):1–11.
  31. Shahsavari M, Norouzi P, Kalalianmoghaddam H, Teimouri M. Effects of Kudzu Root on Oxidative Stress and Inflammation in Streptozotocin-induced Diabetic Rats. Iran J Vet Med. 2023;17(4):401–8.
  32. Cheraghi J, Zargushi M, Khyabani PK, Nasri S. Effects of Parsley (Petroselinum Crispum) Hydroalcoholic Extract on Spermatogenesis and Pituitary-Gonadal Axis in Streptozotocin-Induced Diabetic Male Rat. Iranian Journal of Veterinary Medicine. 15(4):411-422.
  33. Khani A, Khorasgani EM. Investigating the Effect of Hydroalcoholic Extract of Eryngos on Plasma Concentration of Blood Glucose, Blood Cells and Pancreatic Tissue in Diabetic Rats. Iran J Vet Med. 2021;15(4):440–51.
آمار
تعداد مشاهده مقاله: 11,607
تعداد دریافت فایل اصل مقاله: 425


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب