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Saberi, Hassan, Mehranfard, Nasrin, rezazadeh, Hossein, Ghasemi, Maedeh. (1403). Involvement of γ-Aminobutyric acid and N-methyl-D-aspartate receptors in diabetic gastropathy in rats: possible beneficial effect of prolonged treatment with insulin and magnesium supplement. سامانه مدیریت نشریات علمی, 79(3), 601-608. doi: 10.32592/ARI.2024.79.3.601
Hassan Saberi; Nasrin Mehranfard; Hossein rezazadeh; Maedeh Ghasemi. "Involvement of γ-Aminobutyric acid and N-methyl-D-aspartate receptors in diabetic gastropathy in rats: possible beneficial effect of prolonged treatment with insulin and magnesium supplement". سامانه مدیریت نشریات علمی, 79, 3, 1403, 601-608. doi: 10.32592/ARI.2024.79.3.601
Saberi, Hassan, Mehranfard, Nasrin, rezazadeh, Hossein, Ghasemi, Maedeh. (1403). 'Involvement of γ-Aminobutyric acid and N-methyl-D-aspartate receptors in diabetic gastropathy in rats: possible beneficial effect of prolonged treatment with insulin and magnesium supplement', سامانه مدیریت نشریات علمی, 79(3), pp. 601-608. doi: 10.32592/ARI.2024.79.3.601
Saberi, Hassan, Mehranfard, Nasrin, rezazadeh, Hossein, Ghasemi, Maedeh. Involvement of γ-Aminobutyric acid and N-methyl-D-aspartate receptors in diabetic gastropathy in rats: possible beneficial effect of prolonged treatment with insulin and magnesium supplement. سامانه مدیریت نشریات علمی, 1403; 79(3): 601-608. doi: 10.32592/ARI.2024.79.3.601

Involvement of γ-Aminobutyric acid and N-methyl-D-aspartate receptors in diabetic gastropathy in rats: possible beneficial effect of prolonged treatment with insulin and magnesium supplement

Archives of Razi Institute
مقاله 20، دوره 79، شماره 3، مرداد و شهریور 2024، صفحه 601-608    اصل مقاله (507.35 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.3.601
نویسندگان
Hassan Saberi1؛ Nasrin Mehranfard2؛ Hossein rezazadeh3؛ Maedeh Ghasemi* 3
1School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2Neurophysiology Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran
3Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده
Gastrointestinal dysfunction is a severe and common complication in diabetic patients. Some evidence shows that gamma-aminobutyric acid (GABA) and glutamate contribute to diabetic gastrointestinal abnormalities. Hence, we examined the impact of prolonged treatment with insulin and magnesium supplement on the expression pattern of GABA type A (GABA-A), GABA-B and N-methyl-D-aspartate (NMDA) glutamate receptors as well as nitric oxide synthase 1 (NOS-1) in the stomach of type 2 diabetic rats. Twenty-four male Wistar rats were randomized to 4 groups (6 rats each): 1) control, 2) type 2 diabetes: rats fed with a high fat diet for 3 months + low dose of streptozotocin (35 mg/kg), 3) type 2 diabetes + magnesium, and 4) type 2 diabetes + insulin. The expression of NOS-1 as well as GABA-A, GABA-B, and NMDA receptors was detected using western blotting. NOS-1 expression was substantially diminished (p<0.01) while the expression of GABA-A (p<0.001), GABA-B (p<0.001), and NMDA (p<0.001) receptors was enhanced in the stomach of diabetic rats relative to control. Treatment with magnesium and insulin improved NOS-1 expression in diabetic rats, although this effect was greater in magnesium treatment alone. Magnesium also restored the expression of GABA-A and GABA-B receptors in diabetic rats to control values. Moreover, insulin treatment improved GABA-A receptor expression in diabetic rats (p<0.05). No considerable alterations were detected in NMDA receptor level in the treatment groups. The results suggest a significant role of magnesium and insulin in improving gastric motility and secretory disorders associated with diabetes through modifying the expression of GABAergic receptors.
کلیدواژه‌ها
Type 2 Diabetes؛ Magnesium Supplement؛ GABA Receptor؛ NMDA Receptor؛ NOS-1
عنوان مقاله [English]
دخالت رسپتورهای گاما-آمینوبوتیریک اسید و ان-متیل-دی-آسپارتات در گاستروپاتی دیابتی در موش صحرائی: اثرات سودمند درمان بلند مدت انسولین و مکمل منیزیم
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