اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها2,998
تعداد مقالات33,579
تعداد مشاهده مقاله44,251,005
تعداد دریافت فایل اصل مقاله20,621,979
Mahjoub, Mohammad Ali, Iman, Maryam, salimisabour, ebrahim, anbara, hojat. (1403). Evaluation of Anti-Inflammatory Effects of Chitosan film Loaded Arnebia euchroma Extract through In Vitro and In Vivo Studies. سامانه مدیریت نشریات علمی, 79(6), 1271-1280. doi: 10.32592/ARI.2024.79.6.1271
Mohammad Ali Mahjoub; Maryam Iman; ebrahim salimisabour; hojat anbara. "Evaluation of Anti-Inflammatory Effects of Chitosan film Loaded Arnebia euchroma Extract through In Vitro and In Vivo Studies". سامانه مدیریت نشریات علمی, 79, 6, 1403, 1271-1280. doi: 10.32592/ARI.2024.79.6.1271
Mahjoub, Mohammad Ali, Iman, Maryam, salimisabour, ebrahim, anbara, hojat. (1403). 'Evaluation of Anti-Inflammatory Effects of Chitosan film Loaded Arnebia euchroma Extract through In Vitro and In Vivo Studies', سامانه مدیریت نشریات علمی, 79(6), pp. 1271-1280. doi: 10.32592/ARI.2024.79.6.1271
Mahjoub, Mohammad Ali, Iman, Maryam, salimisabour, ebrahim, anbara, hojat. Evaluation of Anti-Inflammatory Effects of Chitosan film Loaded Arnebia euchroma Extract through In Vitro and In Vivo Studies. سامانه مدیریت نشریات علمی, 1403; 79(6): 1271-1280. doi: 10.32592/ARI.2024.79.6.1271

Evaluation of Anti-Inflammatory Effects of Chitosan film Loaded Arnebia euchroma Extract through In Vitro and In Vivo Studies

Archives of Razi Institute
مقاله 18، دوره 79، شماره 6، بهمن و اسفند 2024، صفحه 1271-1280    اصل مقاله (588.3 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.6.1271
نویسندگان
Mohammad Ali Mahjoub1؛ Maryam Iman* 1؛ ebrahim salimisabour2؛ hojat anbara3
1Department of Pharmaceutics, Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran
2Department of Pharmacognosy, Faculty of Pharmacy, Baqiatallah University of Medical Sciences, Tehran, Iran.
3Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
چکیده
This study assessed the anti-inflammatory effects of chitosan (CHT) film loaded with Arnebia euchroma extract, both in vitro and in vivo. Arnebia euchroma contains shikonin (SHKN), a naphthoquinone with significant anti-inflammatory, antimicrobial, and wound-healing properties. High-quality SHKN extract was standardized and incorporated into CHT films, which were then evaluated for stability, drug release, antibacterial effectiveness, and anti-inflammatory activity.
Two concentrations of SHKN were used in CHT film preparations. In vitro studies showed that the optimal CHT film formulation was stable over four weeks at 4°C. A biphasic SHKN release profile from the films was observed, indicative of a sustained drug release mechanism. The films exhibited a strong antibacterial effect against Staphylococcus aureus (S. aureus) due to the presence of SHKN, but no such effect against Escherichia coli (E. coli). A synergistic antibacterial effect was noted when CHT was combined with A. euchroma extract against S. aureus.
In vivo, CHT film with A. euchroma extract showed anti-inflammatory effects in a mice paw swelling test, comparable to betamethasone (mice were divided into 4 groups of 6) the difference was not statistically significant (p value > 0.05). Histological examination confirmed the reduction of immune cell infiltration in the treatment group. The study concluded that CHT films containing A. euchroma extract exhibit promising anti-inflammatory and antimicrobial properties. They are also suitable for use as wound dressings, having high portability, mechanical strength, and being non-adhesive, making them readily applicable for medical and pharmaceutical use, as well as potential carriers for antimicrobial agents and antioxidants in various industries. In conclusion the use of Chitosan films embedded with Arnebia euchroma extract could offer an accessible and convenient therapeutic application for various wounds and inflammatory conditions.
کلیدواژه‌ها
Arnebia euchroma؛ Chitosan؛ Shikonin؛ Anti-inflammatory؛ Anti-bacterial
عنوان مقاله [English]
بررسی اثرات ضد التهابی فیلم کیتوزانی دارای عصاره ابوخلسا به صورت برون تن و درون تن
مراجع
  1. Kumar A, Shashni S, Kumar P, Pant D, Singh A, Verma RKJJoE. Phytochemical constituents, distributions and traditional usages of Arnebia euchroma: A review. 2021;271:113896.
  2. Ashkani-Esfahani S, Imanieh M, Khoshneviszadeh M, Meshksar A, Noorafshan A, Geramizadeh B, et al. The healing effect of arnebia euchroma in second degree burn wounds in rat as an animal model. 2012;14(2):70.
  3. Nasiri E, Hosseinimehr SJ, Hosseinzadeh AZ, Azadbakht M, Akbari J, Azadbakht MJJoe. The effects of Arnebia euchroma ointment on second-degree burn wounds: a randomized clinical trial. 2016;189:107-16.
  4. Liu T, Ma C, Yang L, Wang W, Sui X, Zhao C, et al. Optimization of shikonin homogenate extraction from Arnebia euchroma using response surface methodology. 2013;18(1):466-81.
  5. Huang X-Y, Fu H-L, Tang H-Q, Yin Z-Q, Zhang W, Shu G, et al. Optimization extraction of shikonin using ultrasound-assisted response surface methodology and antibacterial studies. 2020;2020:1-10.
  6. Yadav S, Sharma A, Nayik GA, Cooper R, Bhardwaj G, Sohal HS, et al. Review of shikonin and derivatives: isolation, chemistry, biosynthesis, pharmacology and toxicology. 2022;13:905755.
  7. Wang B, Wu Z, Wang J, Li W, Liu G, Zhang B, et al. Insights into the mechanism of Arnebia euchroma on leukemia via network pharmacology approach. 2020;20(1):1-15.
  8. Jiménez-Gómez CP, Cecilia JAJM. Chitosan: a natural biopolymer with a wide and varied range of applications. 2020;25(17):3981.
  9. Gupta RK, Guha P, Srivastav PPJFCA. Natural polymers in bio-degradable/edible film: A review on environmental concerns, cold plasma technology and nanotechnology application on food packaging-A recent trends. 2022;1:100135.
  10. Kienzle‐Sterzer CA, Rodriguez‐Sanchez D, Rha CJDMCMC, Physics. Mechanical properties of chitosan films: effect of solvent acid. 1982;183(5):1353-9.
  11. Tanaka S, Tajima M, Tsukada M, Tabata MJJoNP. A comparative study on anti-inflammatory activities of the enantiomers, shikonin and alkannin. 1986;49(3):466-9.
  12. Liao P-L, Lin C-H, Li C-H, Tsai C-H, Ho J-D, Chiou GC, et al. Anti-inflammatory properties of shikonin contribute to improved early-stage diabetic retinopathy. 2017;7(1):44985.
  13. Selvamani V. Stability studies on nanomaterials used in drugs. In Staphylococcus aureus Characterization and biology of nanomaterials for drug delivery 2019 Jan 1 (pp. 425-444). Elsevier.
  14. Singh B, Sharma M, Meghwal P, Sahu P, Singh SJP. Anti-inflammatory activity of shikonin derivatives from Arnebia hispidissima. 2003;10(5):375-80.
  15. Shokrzadeh M, Azadbakht M, Shakibamanesh HJP, Research B. The hepatoprotective effect of Arnebia euchroma hydro-alcoholic extract against liver toxicity induced by CCl4 in mice. 2017;3(4):23-9.
  16. Feng H, Zhang L, Zhu CJC, Biointerfaces SB. Genipin crosslinked ethyl cellulose–chitosan complex microspheres for anti-tuberculosis delivery. 2013;103:530-7.
  17. Andújar I, Ríos JL, Giner RM, Recio MCJPm. Pharmacological properties of shikonin–a review of literature since 2002. 2013:1685-97.
  18. Ding X, Yin B, Qian L, Zeng Z, Yang Z, Li H, et al. Screening for novel quorum-sensing inhibitors to interfere with the formation of Pseudomonas aeruginosa biofilm. 2011;60(12):1827-34.
  19. Papageorgiou VP, Assimopoulou AN, Couladouros EA, Hepworth D, Nicolaou KJACIE. The chemistry and biology of alkannin, shikonin, and related naphthazarin natural products. 1999;38(3):270-301.
  20. Shen C-C, Syu W-J, Li S-Y, Lin C-H, Lee G-H, Sun C-MJJoNP. Antimicrobial activities of naphthazarins from arnebia e uchroma. 2002;65(12):1857-62.
  21. Coma V, Deschamps A, Martial‐Gros AJJofs. Bioactive packaging materials from edible chitosan polymer—antimicrobial activity assessment on dairy‐related contaminants. 2003;68(9):2788-92.
  22. De Masi A, Tonazzini I, Masciullo C, Mezzena R, Chiellini F, Puppi D, et al. Chitosan films for regenerative medicine: Fabrication methods and mechanical characterization of nanostructured chitosan films. 2019;11:807-15.
  23. Melro E, Antunes FE, da Silva GJ, Cruz I, Ramos PE, Carvalho F, et al. Chitosan films in food applications. Tuning film properties by changing acidic dissolution conditions. 2020;13(1):1.
  24. Kim S, Nakamatsu J, Maurtua D, Oliveira FJJoAPS. Formation, antimicrobial activity, and controlled release from cotton fibers with deposited functional polymers. 2016;133(8).
  25. M. Ways TM, Lau WM, Khutoryanskiy VVJP. Chitosan and its derivatives for application in mucoadhesive drug delivery systems. 2018;10(3):267.
  26. Yuan Y, Chesnutt BM, Haggard WO, Bumgardner JDJM. Deacetylation of chitosan: Material characterization and in vitro evaluation via albumin adsorption and pre-osteoblastic cell cultures. 2011;4(8):1399-416.
  27. Cheung RCF, Ng TB, Wong JH, Chan WYJMd. Chitosan: an update on potential biomedical and pharmaceutical applications. 2015;13(8):5156-86.
  28. Zhang M, Li X, Gong Y, Zhao N, Zhang XJB. Properties and biocompatibility of chitosan films modified by blending with PEG. 2002;23(13):2641-8.
  29. Ahmed S, Ikram SJAitls. Chitosan based scaffolds and their applications in wound healing. 2016;10(1):27-37.
  30. Kumirska J, Weinhold MX, Thöming J, Stepnowski PJP. Biomedical activity of chitin/chitosan based materials—influence of physicochemical properties apart from molecular weight and degree of N-acetylation. 2011;3(4):1875-901.
  31. Gallo J, Raska M, Kriegova E, Goodman SBJJoot. Inflammation and its resolution and the musculoskeletal system. 2017;10:52-67.
  32. Davydova V, Kalitnik A, Markov P, Volod’Ko A, Popov S, Ermak IJAB, et al. Cytokine-inducing and anti-inflammatory activity of chitosan and its low-molecular derivative. 2016;52:476-82.
  33. Friedman AJ, Phan J, Schairer DO, Champer J, Qin M, Pirouz A, et al. Antimicrobial and anti-inflammatory activity of chitosan–alginate nanoparticles: a targeted therapy for cutaneous pathogens. 2013;133(5):1231-9.
  34. Oliveira MI, Santos SG, Oliveira MJ, Torres AL, Barbosa MAJECM. Chitosan drives anti-inflammatory macrophage polarisation and pro-inflammatory dendritic cell stimulation. 2012;24(136):133-6.
  35. Chung MJ, Park JK, Park YIJIi. Anti-inflammatory effects of low-molecular weight chitosan oligosaccharides in IgE–antigen complex-stimulated RBL-2H3 cells and asthma model mice. 2012;12(2):453-9.
  36. Li Y, Liu H, Xu Q-S, Du Y-G, Xu JJCP. Chitosan oligosaccharides block LPS-induced O-GlcNAcylation of NF-κB and endothelial inflammatory response. 2014;99:568-78.
  37. Younes I, Rinaudo MJMd. Chitin and chitosan preparation from marine sources. Structure, properties and applications. 2015;13(3):1133-74.
  38. Aranaz I, Mengíbar M, Harris R, Paños I, Miralles B, Acosta N, et al. Functional characterization of chitin and chitosan. 2009;3(2):203-30.
  39. Bessa LJ, Fazii P, Di Giulio M, Cellini LJIwj. Bacterial isolates from infected wounds and their antibiotic susceptibility pattern: some remarks about wound infection. 2015;12(1):47-52.
  40. Kim S, Fernandes MM, Matamá T, Loureiro A, Gomes AC, Cavaco-Paulo AJC, et al. Chitosan–lignosulfonates sono-chemically prepared nanoparticles: characterisation and potential applications. 2013;103:1-8.
  41. Silva C, Matamá T, Kim S, Padrão J, Prasetyo EN, Kudanga T, et al. Antimicrobial and antioxidant linen via laccase-assisted grafting. 2011;71(7):713-20.
  42. Park BK, Kim M-MJIjoms. Applications of chitin and its derivatives in biological medicine. 2010;11(12):5152-64.
  43. Zheng L-Y, Zhu J-FJCp. Study on antimicrobial activity of chitosan with different molecular weights. 2003;54(4):527-30.
  44. Liu N, Chen X-G, Park H-J, Liu C-G, Liu C-S, Meng X-H, et al. Effect of MW and concentration of chitosan on antibacterial activity of Escherichia coli. 2006;64(1):60-5.
  45. Kumar-Krishnan S, Prokhorov E, Hernández-Iturriaga M, Mota-Morales JD, Vázquez-Lepe M, Kovalenko Y, et al. Chitosan/silver nanocomposites: Synergistic antibacterial action of silver nanoparticles and silver ions. 2015;67:242-51.
  46. Yin S-Y, Peng A-P, Huang L-T, Wang Y-T, Lan C-W, Yang N-SJE-BC, et al. The phytochemical shikonin stimulates epithelial-mesenchymal transition (EMT) in skin wound healing. 2013;2013.
  47. Ozaki Y, Ohno A, Saito Y, Satake MJB, Bulletin P. Accelerative effect of shikonin, alkannin and acetylshikonin on the proliferation of granulation tissue in rats. 1994;17(8):1075-7.
  48. Zhang Y, Han H, Sun L, Qiu H, Lin H, Yu L, et al. Antiviral activity of shikonin ester derivative PMM-034 against enterovirus 71 in vitro. 2017;50.
آمار
تعداد مشاهده مقاله: 199
تعداد دریافت فایل اصل مقاله: 246


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب