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Najafi, Mahdiyeh, Khordadmehr, Monireh, baradaran, Behzad, Najafi, Souzan, Amini, Mohammad, Asadpour, Reza. (1403). The effects of stem cell-conditioned media on malignancy behavior of breast cancer cells in vitro. سامانه مدیریت نشریات علمی, 79(6), 1249-1256. doi: 10.32592/ARI.2024.79.6.1249
Mahdiyeh Najafi; Monireh Khordadmehr; Behzad baradaran; Souzan Najafi; Mohammad Amini; Reza Asadpour. "The effects of stem cell-conditioned media on malignancy behavior of breast cancer cells in vitro". سامانه مدیریت نشریات علمی, 79, 6, 1403, 1249-1256. doi: 10.32592/ARI.2024.79.6.1249
Najafi, Mahdiyeh, Khordadmehr, Monireh, baradaran, Behzad, Najafi, Souzan, Amini, Mohammad, Asadpour, Reza. (1403). 'The effects of stem cell-conditioned media on malignancy behavior of breast cancer cells in vitro', سامانه مدیریت نشریات علمی, 79(6), pp. 1249-1256. doi: 10.32592/ARI.2024.79.6.1249
Najafi, Mahdiyeh, Khordadmehr, Monireh, baradaran, Behzad, Najafi, Souzan, Amini, Mohammad, Asadpour, Reza. The effects of stem cell-conditioned media on malignancy behavior of breast cancer cells in vitro. سامانه مدیریت نشریات علمی, 1403; 79(6): 1249-1256. doi: 10.32592/ARI.2024.79.6.1249

The effects of stem cell-conditioned media on malignancy behavior of breast cancer cells in vitro

Archives of Razi Institute
مقاله 15، دوره 79، شماره 6، بهمن و اسفند 2024، صفحه 1249-1256    اصل مقاله (428.89 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.6.1249
نویسندگان
Mahdiyeh Najafi1؛ Monireh Khordadmehr* 1؛ Behzad baradaran2؛ Souzan Najafi3؛ Mohammad Amini3؛ Reza Asadpour4
1Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, 51665-1647, Tabriz, Iran
2Immunology Research Center, Tabriz University of Medical Sciences, 51666-14761, Tabriz, Iran, and Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, 51666-14761, Tabriz, Iran
3Immunology Research Center, Tabriz University of Medical Sciences, 51666-14761, Tabriz, Iran
4Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, 51665-1647, Tabriz, Iran
چکیده
Breast cancer is the most commonly diagnosed cancer among women worldwide. In recent years, there has been growing interest among researchers in exploring alternative therapeutic methods, including stem cell therapy. Therefore, this study aimed to investigate the effects of adipose-derived mesenchymal stem cell-conditioned media (AD-MSCs-CM) on apoptosis induction and migration inhibition of breast cancer cells (MDA-MB-231) in vitro. Here, malignant breast cancer cells (MDA-MB-231) and adipose mesenchymal stem cells (AD-MSC) were cultured separately in DMEM F12/FBS15% culture media in standard conditions. Subsequently, the conditioned media derived from AD-MSCs was exposed to the MDA-MB-231 cells. After 24 and 48 hours of exposure, the expression levels of CASP3, KRAS, and MMP9, were assessed using a qRT-PCR assay. Additionally, the proliferation and migration abilities of the cancer cells were evaluated using MTT and wound healing assays, respectively. Also, the protein expression of Caspase-3, K-RAS, and MMP-9 was examined using the western blot assay. Notably, the expression level of MMP9 and KRAS genes significantly decreased following AD-MSCs-CM treatment in MDA-MB-231 cells. Moreover, the expression level of CASP3 gene remarkably increased in the treated groups. Besides, the proliferation of MDA-MB-231 cells treated with AD-MSCs-CM was strongly reduced by MTT and wound healing assays. Furthermore, the AD-MSCs-CM demonstrated an increase in the activation of Caspase-3 and a decrease in the protein expressions of K-RAS and MMP-9. The findings of this study suggest that the AD-MSCs-CM have the potential to influence apoptosis, proliferation, and migration of breast cancer cells. Thus, it could be considered a promising therapeutic strategy for suppressing breast cancer. However, further testing and research are required to validate these findings and explore the full potential of this approach.
کلیدواژه‌ها
Breast cancer؛ Stem cells؛ Apoptosis؛ Migration؛ Gene expression
عنوان مقاله [English]
بررسی اثر محیط کشت سلول های بنیادی مزانشیمی بر رفتار بدخیمی سلول های سرطان پستان در شرایط آزمایشگاهی
چکیده [English]
سرطان پستان شایع ترین سرطان تشخیص داده شده در بین زنان در سراسر جهان است. در سال‌های اخیر، علاقه فزاینده‌ای در میان محققان به بررسی روش‌های درمانی جایگزین، از جمله درمان با سلول‌های بنیادی، وجود داشته است. بنابراین، این مطالعه با هدف بررسی اثرات محیط کشت سلولهای بنیادی بر سلول های بنیادی مزانشیمی مشتق از چربی (AD-MSCs-CM) بر القای آپوپتوز و مهار مهاجرت سلول های سرطان پستان (MDA-MB-231) در شرایط آزمایشگاهی انجام شد. در این مطالعه، سلول های سرطانی بدخیم پستان (MDA-MB-231) و سلول های بنیادی مزانشیمی چربی (AD-MSC) به طور جداگانه در محیط های کشت DMEM F12/FBS15% در شرایط استاندارد کشت داده شدند. متعاقبا، محیط شرطی شده مشتق شده از سلول های بنیادی مزانشیمی AD-MSC در معرض سلول های MDA-MB-231 قرار گرفت. پس از 24 و 48 ساعت قرار گرفتن در معرض، سطح بیان CASP3، KRAS و MMP9 با استفاده از روش qRT-PCR ارزیابی شد. علاوه بر این، توانایی‌های تکثیر و مهاجرت سلول‌های سرطانی به ترتیب با استفاده از روش‌های MTT و تست خراش مورد ارزیابی قرار گرفت. همچنین بیان پروتئین کاسپاز-3، K-RAS و MMP-9 با استفاده از روش وسترن بلات مورد بررسی قرار گرفت. قابل ذکر است که سطح بیان ژن های MMP9 و KRAS به دنبال درمان AD-MSCs-CM در سلول های MDA-MB-231 به طور قابل توجهی کاهش یافت. همچنین میزان بیان ژن CASP3 در گروه‌های آزمایش به‌طور قابل‌توجهی افزایش یافت. علاوه بر این، تکثیر سلول‌های MDA-MB-231 تحت درمان با AD-MSCs-CM توسط روش‌های MTT و تست خراش به شدت کاهش یافت. علاوه بر این، AD-MSCs-CM افزایش در فعال سازی کاسپاز-3 و کاهش بیان پروتئین K-RAS و MMP-9 را نشان داد. یافته‌های این مطالعه نشان می‌دهد که AD-MSCs-CM پتانسیل تأثیرگذاری بر آپوپتوز، تکثیر و مهاجرت سلول‌های سرطان پستان را دارد. بنابراین، می توان آن را یک استراتژی درمانی امیدوارکننده برای سرکوب سرطان پستان در نظر گرفت. با این حال، برای تأیید این یافته‌ها و کشف پتانسیل کامل این رویکرد، آزمایش‌ها و تحقیقات بیشتری لازم است.
کلیدواژه‌ها [English]
سرطان پستان, سلول بنیادی, آپپتوز, مهاجرت, بیان ژن
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