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Effects of temperature stress on genome-wide DNA methylation levels in the sea cucumber, Apostichopus japonicus | ||
| Iranian Journal of Fisheries Sciences | ||
| دوره 23، شماره 3، مرداد 2024، صفحه 437-456 اصل مقاله (902.32 K) | ||
| شناسه دیجیتال (DOI): 10.22092/ijfs.2024.131219 | ||
| نویسندگان | ||
| P. Gao؛ L. Li؛ L. Han؛ Y. Zhang؛ Z. Quan؛ B. Ding؛ Y. Sun؛ B. Han* ؛ J. Ding* | ||
| Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, 116023, China | ||
| چکیده | ||
| Sea cucumber Apostichopus japonicus lives in the lower temperature of the seawater and will enter the state of aestivation when the temperature is high. In recent years, the continuous high temperatures in summer have brought great loss to the production of sea cucumber in north China. To study the epigenetic regulating mechanism of A. japonicus and guide the temperature-controlled culture, the methylation level of A. japonicus genome was detected. Breeding imitation A. japonicus under different temperature conditions for a period of time, the difference of methylation level between the set temperature group and the control group was detected and verified. The methylation levels of A. japonicus in different temperature groups were detected. The methylation sites ranged from 84,766 to 96,225, and the sequencing depth was between 42.33 and 153.21. Under the temperature stress, more than 60% of the differentiated methylated genes in different groups were down-regulated, the HBW group had the most differentiated methylated genes (1,405), and the SBW group had the least differentiated methylated genes (643). Gene function classification and enrichment analysis were carried out, and a total of 3,512 differential genes were found, mostly related to immunity and metabolism. The results showed that temperature can change the gene methylation level of A. japonicus, affecting its expression level, and ultimately changing the metabolic and immune processes of A. japonicus. | ||
| کلیدواژهها | ||
| Apostichopus japonicus؛ Temperature stress؛ Genomic methylation؛ Methylated differential genes | ||
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