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Wang, H., Ding, H.. (1403). A confirmatory method of tetracycline antibiotics in Chinese mitten crab (Eriocheir sinensis) with ultra-performance liquid chromatography tandem high-resolution mass spectrometry. سامانه مدیریت نشریات علمی, 23(4), 575-587. doi: 10.22092/ijfs.2024.131274
H. Wang; H. Ding. "A confirmatory method of tetracycline antibiotics in Chinese mitten crab (Eriocheir sinensis) with ultra-performance liquid chromatography tandem high-resolution mass spectrometry". سامانه مدیریت نشریات علمی, 23, 4, 1403, 575-587. doi: 10.22092/ijfs.2024.131274
Wang, H., Ding, H.. (1403). 'A confirmatory method of tetracycline antibiotics in Chinese mitten crab (Eriocheir sinensis) with ultra-performance liquid chromatography tandem high-resolution mass spectrometry', سامانه مدیریت نشریات علمی, 23(4), pp. 575-587. doi: 10.22092/ijfs.2024.131274
Wang, H., Ding, H.. A confirmatory method of tetracycline antibiotics in Chinese mitten crab (Eriocheir sinensis) with ultra-performance liquid chromatography tandem high-resolution mass spectrometry. سامانه مدیریت نشریات علمی, 1403; 23(4): 575-587. doi: 10.22092/ijfs.2024.131274

A confirmatory method of tetracycline antibiotics in Chinese mitten crab (Eriocheir sinensis) with ultra-performance liquid chromatography tandem high-resolution mass spectrometry

Iranian Journal of Fisheries Sciences
دوره 23، شماره 4، مهر 2024، صفحه 575-587    اصل مقاله (477.03 K)
شناسه دیجیتال (DOI): 10.22092/ijfs.2024.131274
نویسندگان
H. Wang* 1؛ H. Ding2
1Instrumental Analysis Center, Yancheng Teachers University, 224007, Yancheng, China
2College of Pharmacy, Yancheng Teachers University, 224007, Yancheng, China
چکیده
A confirmatory method for the determination of tetracycline antibiotics (TCs) in Chinese mitten crab has been developed for the first time. The method comprises two stages: pretreatment of the samples and instrumental analysis. The samples are treated with Na2EDTA–McIlvaine buffer followed by extraction. The extract is then analyzed with ultra-performance liquid chromatography tandem high-resolution mass spectrometry (UPLC-HRMS). Four mostly used TCs, including tetracycline, oxytetracycline, chlortetracycline, and doxycycline, were detected in 10 min. The specificity was confirmed via the retention time and their high-resolution mass spectra. In the range of 50-500 μg/kg, the linear regression coefficients (R2) for all analytes were greater than 0.9985. Recoveries at three concentration levels were between 90.3% and 106.2%. The within-laboratory reproducibility was precise. The decision limit (CCα) and detection capability (CCβ) of the method were determined using the permitted limit (100 μg/kg) of TCs. The detection results of TCs in 12 batches of Chinese mitten crab showed that no target residues were detected. This comprehensive method is compliant with the 2002/657/EC decision. It could be applied for the determination of TCs in Chinese mitten crab samples and potentially in other aquatic products.
کلیدواژه‌ها
Tetracycline؛ Confirmatory method؛ Chinese mitten crab؛ UPLC؛ HR-MS
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