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Abdalfatah, Mariwan Fathalla, Hjazi, Ahmed, Saravani, Khadijeh, Hassanshahian, Mehdi, Bayat, Zeynab, Soheil beigie, Gelareh. (1403). Screening of biofilm-producing genes from Acinetobacter isolates obtained Covid-19 patients in ICU hospital section. سامانه مدیریت نشریات علمی, 80(1), 147-152. doi: 10.32592/ARI.2025.80.1.147
Mariwan Fathalla Abdalfatah; Ahmed Hjazi; Khadijeh Saravani; Mehdi Hassanshahian; Zeynab Bayat; Gelareh Soheil beigie. "Screening of biofilm-producing genes from Acinetobacter isolates obtained Covid-19 patients in ICU hospital section". سامانه مدیریت نشریات علمی, 80, 1, 1403, 147-152. doi: 10.32592/ARI.2025.80.1.147
Abdalfatah, Mariwan Fathalla, Hjazi, Ahmed, Saravani, Khadijeh, Hassanshahian, Mehdi, Bayat, Zeynab, Soheil beigie, Gelareh. (1403). 'Screening of biofilm-producing genes from Acinetobacter isolates obtained Covid-19 patients in ICU hospital section', سامانه مدیریت نشریات علمی, 80(1), pp. 147-152. doi: 10.32592/ARI.2025.80.1.147
Abdalfatah, Mariwan Fathalla, Hjazi, Ahmed, Saravani, Khadijeh, Hassanshahian, Mehdi, Bayat, Zeynab, Soheil beigie, Gelareh. Screening of biofilm-producing genes from Acinetobacter isolates obtained Covid-19 patients in ICU hospital section. سامانه مدیریت نشریات علمی, 1403; 80(1): 147-152. doi: 10.32592/ARI.2025.80.1.147

Screening of biofilm-producing genes from Acinetobacter isolates obtained Covid-19 patients in ICU hospital section

Archives of Razi Institute
مقاله 17، دوره 80، شماره 1، فروردین و اردیبهشت 2025، صفحه 147-152    اصل مقاله (398.05 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.1.147
نویسندگان
Mariwan Fathalla Abdalfatah1؛ Ahmed Hjazi2؛ Khadijeh Saravani3؛ Mehdi Hassanshahian* 4؛ Zeynab Bayat4؛ Gelareh Soheil beigie5
1Department of Medical Laboratory, Kurdistan Technical Institute, Sulaymaniyah, Kurdistan Region, Iraq
2PhD in Clinical Medicine, Department of Medical Laboratory, College of Applied Medical Sciences, Prince Stattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia
3Department of community Medicine, School of Medicine, Pediatric Gastroenterology and Hepatology Research Center, Zabol University of Medical Science, Zabol, Iran
4Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran
5PhD Student in microbiology, Islamic Azad University of Ayatollah Amoli, Amol, Iran
چکیده
The corona pandemic caused a large number of people to be admitted to the intensive care units of hospitals. Due to the special closed system of these parts and the lack of proper air circulation, Spire equipment is a suitable place for the accumulation of commensal bacteria and the creation of biofilm. Biofilm is actually a collection of microbes that stabilize on a surface. Bacteria in biofilm structures have relationships with each other and their behavior is different from when they are alone. The resistance of bacteria to antimicrobial agents in the biofilm state is different from the planktonic state. Many genes are involved in the formation of biofilm, these genes act in a cascade manner and the activity of one gene depends on the activity of another gene
Acinetobacter, recognized as a nosocomial pathogen, undergoes structural changes when exposed to various antibiotics, rendering it relatively resistant and posing challenges in disease treatment. This study aimed to identify two biofilm-related genes and assess the drug resistance profile of clinical strains. Clinical isolates were collected from the ICU of Afzalipour Hospital in Kerman, Iran, and phenotypically identified. Confirmation was achieved for 55 clinical Acinetobacter isolates. Antibiogram testing was conducted for meropenem, amikacin, ampicillin-sulbactam, cefotaxime, levofloxacin, rifampin, and tigecycline antibiotics. Biofilm formation ability was assessed using microtiter plates and crystal violet staining, followed by spectrophotometry at OD 490 nm. PCR was employed to determine the frequency of pslA and pelB genes. Analysis revealed that the highest age group affected was 1 to 15 years (19%), while the lowest was 26 to 35 years (5%). The frequencies of pslA and pelB genes were 34.5% and 65.5%, respectively, and drug resistance ranged from 72% to 100% for the mentioned antibiotics. Given the pelB gene's approximately twofold higher frequency compared to pslA, it suggests that in most studied isolates, Psl may often be disrupted or that intracellular c-di-GMP levels have significantly increased.
کلیدواژه‌ها
Biofilms؛ Acinetobacter؛ Drug Resistance؛ Antibiotic؛ pslA and pelB genes
عنوان مقاله [English]
غربالگری ژن‌های تولیدکننده بیوفیلم از ایزوله‌های اسینتوباکتر به‌دست‌آمده از بیماران کووید-19 در بخش ICU بیمارستان
چکیده [English]
سابقه و هدف: اسینتوباکتر که یک عامل عفونی بیمارستانی محسوب می شود، به دلیل قرار گرفتن در معرض آنتی بیوتیک های مختلف، ساختار خود را تغییر داده و نسبت به آنها مقاوم می شود که درمان بیماری های ناشی از این باکتری را دشوار می کند.
اپیدمی کرونا که از سال 2019 در جهان آغاز شد، منجر به افزایش تعداد بیماران در بخش ICU بیمارستان ها شد. ویروس کرونا به دلیل درگیری دستگاه تنفسی مشکلات زیادی را برای بیماران ایجاد کرد
هدف: این مطالعه با هدف شناسایی دو ژن سازنده بیوفیلم و همچنین مشخصات مقاومت دارویی سویه های بالینی انجام شد.
روش کار: در این مطالعه، ایزوله های بالینی از بیمارستان افضلی پور کرمان جمع آوری و به عنوان فنوتیپی شناسایی شدند. 55 ایزوله بالینی اسینتوباکتر تایید شد. آزمایش آنتی بیوگرام برای آنتی بیوتیک های مروپنم، آمیکاسین، آمپی سیلین-سولباکتام، سفوتاکسیم، لووفلوکساسین، ریفامپین و تیژسیکلین انجام شد. توانایی تشکیل بیوفیلم جدایه ها با استفاده از پلیت میکروتیتر و رنگ آمیزی با استفاده از کریستال ویولت و سپس اسپکتروفتومتری در OD 490 نانومتر تعیین شد. PCR برای شناسایی فراوانی ژن های pslA و pelB انجام شد.
یافته ها: داده ها نشان داد که بیشترین گروه سنی 1 تا 15 سال شامل 19 درصد و کمترین گروه سنی 26 تا 35 سال شامل 5 درصد بود. فراوانی ژن های pslA و pelB به ترتیب 5/34 و 5/65 درصد و دامنه مقاومت دارویی برای آنتی بیوتیک های مذکور بین 72 تا 100 درصد بود.
نتیجه‌گیری: از آنجایی که فراوانی ژن pelB حدود دو برابر pslA است، می‌توان نتیجه گرفت که در اکثر جدایه‌های مورد مطالعه، Psl اغلب دارای اختلال است یا سطح داخل سلولی c-di-GMP به شدت افزایش یافته است. .
کلیدواژه‌ها [English]
بیوفیلم, اسینتوباکتر, مقاومت دارویی, انتی بیوتیک, ژنهای PslA
مراجع
  1. Al-Rashedi, N. A., Alburkat, H., Hadi, A. O., Munahi, M. G., Jasim, A., Hameed, A, Smura, T. High prevalence of an alpha variant lineage with a premature stop codon in ORF7a in Iraq, winter 2020–2021. PLoS One. 2022;17(5):e0267295.
  2. Qasemi A, Bayat Z, Akbari N, Babazadeh D. Bacterial Resistance of Acinetobacter baumannii: A Global Concern. Research in Biotechnology and Environmental Science. 2022;1(2): 36-42.
  3. Bahrami Nejad Joneghani R, Bahrami Nejad Joneghani R, Dustmohammadloo H, Bouzari P, Ebrahimi P, Fekete-Farkas M. Self-Compassion, Work Engagement and Job Performance among Intensive Care Nurses during COVID-19 Pandemic: The Mediation Role of Mental Health and the Moderating Role of Gender. InHealthcare. MDPI. 2023;11(13):1884.
  4. Salehinasab A, Sichani AR, Mousavi M, Bayat Z, Pezhhan A, Hussien BM, Ahmed M, Hassanshahian M. Investigation of Microbial Biofilms during COVID-19 Pandemic: A Bibliometric Analysis. Iranian Red Crescent Medical JournaL 2023;25(9).
  5. Al-Rashedi, N. A., Alburkat, H., Munahi, M. G., Jasim, A. H., Salman, B. K., Oda, B. S, Smura, T. Genome Sequence of an Early Imported Case of SARS-CoV-2 Delta Variant (B. 1.617. 2 AY. 122) in Iraq in April 2021. Microbiology Resource Announcements. 2022;11(11):e00977-22.
  6. Al-Rashedi, N. A., Munahi, M. G., & Ah ALObaidi, L. Prediction of potential inhibitors against SARS-CoV-2 endoribonuclease: RNA immunity sensing. Journal of Biomolecular Structure and Dynamics. 2022;40(11), 4879-4892.
  7. Hussain, S, Sheikh, N, Anjum, M., Raza, A. G, Rizvi, R. Mathematical modelling of COVID-19 pandemic in Pakistan with optimal control. Journal of Asian Scientific Research. 2023;13(1), 28–44.
  8. Luque- Ramos, L., Vilca, J. A., Pilco, A. V., & Cachicatari -Vargas, E. (2024). Events allegedly attributable to vaccination and immunization of COVID-19 in people who received up to the third dose, Tacna-Peru. Nurture. 2022;18(1):91–102.
  9. Davoudi-Monfared E, Khaje-Mozafari J, Keramatinia A, Naimi E, Amiri P, Rahimpour E, et al. A Review on Lifestyle Before and After COVID-19 Pandemic: Four Levels of Prevention: Lifestyle before and after COVID-19 pandemic. Int J Body Mind Cult. 11(2).
  10. Saberi-Hamedani M, Amiri P, Keramatinia A, Shahrbaf MA, Shekarriz-Foumani R. The Prediction of Suicide Ideation Based on Perceived Social Support, Personality Traits, and Meaning of Life in Medical Students during COVID-19 Pandemic: A Cross-Sectional Study: Suicide ideation prediction in medical students. Int J Body Mind Cult. :272–281.
  11. Hashemikamangar SS, Afshari A, Aghamir ZS, Kamali F. Impact of Oral Health Literacy and COVID-19 Induced Anxiety on Dentistry Visits of the Iranian Public: COVID-19 induced anxiety and dentistry visits. Int J Body Mind Cult. :366–373.
  12. Motavaselian M, Farrokhi M, Jafari Khouzani P, et al. Diagnostic Performance of Ultrasonography for Identification of Small Bowel Obstruction; a Systematic Review and Meta-analysis. Arch Acad Emerg Med. 2024; 12(1): e33.
  13. Shamabadi, A., Karimi, H., Arabzadeh Bahri, R., Motavaselian, M., & Akhondzadeh, S. Emerging drugs for the treatment of irritability associated with autism spectrum disorder. Expert Opinion on Emerging Drugs, (just-accepted). 2024.
  14. Saadi, M. I., Nikandish, M., Ghahramani, Z., Valandani, F. M., Ahmadyan, M., Hosseini, F, Ramzi, M. miR-155 and miR-92 levels in ALL, post-transplant aGVHD, and CMV: possible new treatment options. Journal of the Egyptian National Cancer Institute. 2023;35(1), 18.
  15. Iravani Saadi M, Jiang M, Banakar M, Mardani Valandani F., Ahmadyan M., Rostamipour HA, Hosseini F. Are the Costimulatory Molecule Gene Polymorphisms (CTLA-4) Associated With Infection in Organ Transplantation? A Meta-Analysis. Cell Transplantation. 2023.
  16. Khalafi-Nezhad A, Saadi MI., Noshadi N, Jalali H, Ahmadyan M, Kheradmand N, Hamidieh, AA. Change in Programmed Death-1 and Inducible Costimulator Expression in Patients with Acute Myeloid Leukemia Following Chemotherapy and Its Cytogenetic Abnormalities. Galen Medical Journal. 2022;11:e2394-e2394.
  17. Hjazi, A. The effects of Capsicum annuum supplementation on lipid profiles in adults with metabolic syndrome and related disorders: A systematic review and meta‐analysis of randomized controlled trials. Phytotherapy Research. 2023.
  18. Al-Obaidi ZMJ, Hussain YA, Ali AA, Al-Rekabi MD. The influence of vitamin-C intake on blood glucose measurements in COVID-19 pandemic. J Infect Dev Ctries. 2021;15(2):209-213.
  19. Lattef Ismaeel, Z. A. Anatomical study with antibacterial and antibiofilm effects of Erodium cicutarium (L.) L'H phenolic roots extract. Caspian Journal of Environmental Sciences. 2024;22(1), 137-146.
  20. Ahmed M., K. Resen, A, S. AL-Niaeem, K. (). Effects of antibiotic residues on some health parameters of Planiliza abu H. in Shatt Al-Arab, Southern Iraq. Caspian Journal of Environmental Sciences. 2022;20(5):1059-1068.
  21. Obaid, R. F., Kadhim Hindi, N. K., Kadhum, S. A., jafaar alwaeli, LA, Jalil, AT. Antibacterial activity, anti-adherence and anti-biofilm activities of plants extracts against Aggregatibacter actinomycetemcomitans: An in vitro study in Hilla City, Iraq. Caspian Journal of Environmental Sciences, 2022;20(2):367-372.
  22. Stepanović S, Vuković D, Dakić I, Savić B, Švabić-Vlahović M. A modified microtiter-plate test for quantification of staphylococcal biofilm formation. Journal of microbiological methods. 2000;40(2):175-9.
  23. Garcia KCdOD, de Oliveira Corrêa IM, Pereira LQ, Silva TM, Mioni MdSR, de Moraes Izidoro AC, et al. Bacteriophage use to control Salmonella biofilm on surfaces present in chicken slaughterhouses. Poultry science. 2017;96(9):3392-8.
  24. Sekiguchi J-I, Asagi T, Miyoshi-Akiyama T, Kasai A, Mizuguchi Y, Araake M, et al. Outbreaks of multidrug-resistant Pseudomonas aeruginosa in community hospitals in Japan. Journal of clinical microbiology. 2007;45(3):979-89.
  25. Nikbakht, G. Novel Insights into Infection and immunity. Iranian Journal of Veterinary Medicine, 2022; 16(2): 99-100.
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