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Allahverdi Mamaghani, Bahareh, Hesamzadeh Hejazi, Seyed Mohsen, Mirza, Mehdi, Movafeghi, Ali. (1403). Comparative Analysis of the Essential Oils of Dracocephalum moldavica L. from Greenhouse and In vitro Cultured Conditions. سامانه مدیریت نشریات علمی, 14(1), 69-79. doi: 10.22034/jmpb.2024.366414.1722
Bahareh Allahverdi Mamaghani; Seyed Mohsen Hesamzadeh Hejazi; Mehdi Mirza; Ali Movafeghi. "Comparative Analysis of the Essential Oils of Dracocephalum moldavica L. from Greenhouse and In vitro Cultured Conditions". سامانه مدیریت نشریات علمی, 14, 1, 1403, 69-79. doi: 10.22034/jmpb.2024.366414.1722
Allahverdi Mamaghani, Bahareh, Hesamzadeh Hejazi, Seyed Mohsen, Mirza, Mehdi, Movafeghi, Ali. (1403). 'Comparative Analysis of the Essential Oils of Dracocephalum moldavica L. from Greenhouse and In vitro Cultured Conditions', سامانه مدیریت نشریات علمی, 14(1), pp. 69-79. doi: 10.22034/jmpb.2024.366414.1722
Allahverdi Mamaghani, Bahareh, Hesamzadeh Hejazi, Seyed Mohsen, Mirza, Mehdi, Movafeghi, Ali. Comparative Analysis of the Essential Oils of Dracocephalum moldavica L. from Greenhouse and In vitro Cultured Conditions. سامانه مدیریت نشریات علمی, 1403; 14(1): 69-79. doi: 10.22034/jmpb.2024.366414.1722

Comparative Analysis of the Essential Oils of Dracocephalum moldavica L. from Greenhouse and In vitro Cultured Conditions

Journal of Medicinal plants and By-products
مقاله 9، دوره 14، شماره 1، فروردین و اردیبهشت 2025، صفحه 69-79    اصل مقاله (910.53 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22034/jmpb.2024.366414.1722
نویسندگان
Bahareh Allahverdi Mamaghani1؛ Seyed Mohsen Hesamzadeh Hejazi* 2؛ Mehdi Mirza3؛ Ali Movafeghi1
1Faculty of Natural science, University of Tabriz, Tabriz, Iran
2Department of Biotechnology, Research Institute of Forest and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
3Department of Medicinal Plants and By-products, Research Institute of Forest and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
چکیده
Dracocephalum moldavica L. is an aromatic annual plant belongs to the Lamiaceae family. A study was conducted to analyze the essential oils of this plant from six population cultivated in a greenhouse and four population under invitro culture conditions. The essential oils were isolated using the hydro-distillation method and Clevenger apparatus. Gas Chromatography (GC) and Gas Chromatography/Mass spectrometry (GC-MS) were used for analysis. The identified components constituted approximately 91.35-96.58% of the essential oil composition under greenhouse conditions and 90.73-98.3% under in vitro culture conditions. The highest essential oil percentage (0.27%) was found in the Karaj (2) population under greenhouse conditions, while the maximum essential oil yield under in vitro culture was 0.1% in the Hamedan (2) population. The main components identified were Neral, Geraniol, Geranial, and Geranyl acetate. Hamedan (2) (greenhouse) had the highest Neral (27.05%) content, Karaj (3) (in vitro culture) had the highest Geraniol (34.32%) content, Karaj (2) (in-vitro culture) had the highest Geranial (56.12%) content, and Karaj (1) (greenhouse) had the highest geranyl acetate (24.75%) content. In conclusion, the content of Neral and Geranyl acetate increased under greenhouse conditions, while the maximum values of Geraniol and Geranial percentage were observed in the essential oils of in vitro culture. The results suggested that both the culture condition and the origin of the population influenced the essential oil percentage and chemical constituents of D. moldavica species.
کلیدواژه‌ها
Dracocephalum moldavica L. Gas chromatography؛ Neral؛ Geraniol؛ Geranial and Geranyl acetate؛ Culture condition
مراجع
  1. Maham M., Akbari H., Delazar A. Chemical Composition and Antinociceptive Effect of the Essential Oil of Dracocephalum moldavica L. Pharmaceut. Sci. 2013; 18(4), 187-192.
  2. Nikitina A.S., Popva O.I., Ushakova L.S., Chumakova V.V., Ivanova L. Studies of the essential oil Dracocephalum moldavica L. cultivated in the Stavropol reigion. Pharmaceut. Chem. J. 2008; 42 (8): 35-39.
  3. Said-Al Ahl H.A.H., Sabra A.S., El Gendy A.N.G., Aziz E.E., Tkachenko K.G. Changes in content and chemical composition of Dracocephalum moldavica L. essential oil at different harvest dates. J. Med. Plants Stud. 2015; 3(2): 61-64.
  4. Aćimović M., Sikora V., Brdar-Jokanović M., Kiprovski B., Popović V. Koren A., Puvača N. Dracocephalum moldovica: Cultivation, Chemical composition and biological activity. J. Agron. Technol. Engineer. Manag. 2019; 2(1): 153-167.
  5. Jamzad Z. Flora of Iran. Research Institute of Forest and Rangelands publication. 2012; 1066p.
  6. Dastmalchi K., Damien-Dorman H.J., Laakso I., Hiltunen R. Chemical composition and antioxidative activity of Moldavian balm (Dracocephalum moldavica L.) extracts. LWT 2007; 40: 1655–1663.
  7. Zargari A. Medicinal Plants (vol 4). Tehran university publication. 1997; 969p.
  8. Sonboli A., Salehi P., Gharehnaghadeh S. Chemical variability in the essential oil composition of Salvia hypoleuca. An endemic species from Iran. J. Essent. Oil Res. 2016; 28 (5): 421-427.
  9. Ehsani A., Alizadeh O., Hashemi M., Afshari A., Aminzare M. Phytochemical, antioxidant and antibacterial properties of Melissa officinalis and Dracocephalum moldavica essential oils. Vet. Res. Forum 2017; 8 (3) 223-229.
  10. A´cimovi´c M., Šovljanski O., Šeregelj V., Pezo, L., Zheljazkov V.D., Ljuji´C J., Tomi´C. A., Cetkovi´c G., Canadanovi´c-Brunet J., Miljkovi´c A. Chemical Composition, Antioxidant, and Antimicrobial Activity of Dracocephalum moldavica L. Essential Oil and Hydrolate. Plants 2022; 11: 941.
  11. Olha B., Antonina P., Mariia Sh. Chemical compositions and sedative activities of the Dracocephalum moldavica L. and Ocimum americanum L. essential oil. Pharmacol. Online 2021; 2: 179-187.
  12. Jiménez Zúñiga M.I., Hurtado Mariles A.J., Castrejón Flores J.L., Mondragón Herrera J.A., Ramírez Sotelo M.G., Cerón Montes G.I. Antidepressant-Like Effects of Dracocephalum moldavica L. in Mouse Models of Immobility Tests. Pharmacog. J. 2019; 11(5): 976-983.
  13. Mafakheri S., Hallaj R., AsghariB. Study on phytochemical and antioxidant properties of dragonhead (Dracocephalum moldavica L.) seed oil, ethanoland aqueous extracts. Iranian J. Med. Aromatic Plant Res. 2022; 38 (1): 176-189.
  14. Racz G., Tibori G., Csedo C. Composition of volatile oil from Dracocephalum moldavica L. Farmacia 1978;26: 93-96.
  15. Holm Y., Galambosi B., Hiltunen R. Variation of the main terpenes in dragonhead (Dracocephalum moldavica L.) during growth. Flav. Fragr. J. 1988; 3 (3): 113-115.
  16. Omidbaigi R., Borna F. Borna T., Inotai, K. Sowing dates affecting on the essential oil content of dragonhead (Dracocephalum moldavica L.) and its constituents. J. Essent. Oil Bear. Plants 2009; 12(5): 580-585.
  17. Mafakheri S., Omidbaigi R., Sefidkon F., Rejali, F. Influence of biofertilizers on the essential oil content and constituents of Dracocephalum moldavica L. J. Essent. Oil Bear. Plants 2012; 15(1): 58-65.
  18. Aziz E.E., El-Sherbeny S. E. Effect of some micro-nutrients on growth and chemical constituents of Sideritis montana as a new plant introduced into Egypt. Arab. Univ. J. Agric. Sci. Ain Shams Univ. Cairo. 2003; 12: 391-403.
  19. Kakasy A.Z., Lemberkovics E., Simandi B., Lelik L., Hethelyi E., Antal I., Szoke E. Comparative study of traditional essential oil and supercritical fluid extracts of moldavian dragonhead (Dracocephalum moldavica L.). Flav. Fragr. J. 2006; 21(4): 598-603.
  20. Yousefzadeh S., Modarres-Sanavy S.A., Sefidkon F., Asgarzadeh A., Ghalavand A., Sadat-Asilan K. Effects of azocompost and ureaon the herbage yield and contents and compositions of essential oils from two genotypes of dragonhead (Dracocephalum moldavica L.) in two regions of Iran. Food Chem. 2013; 138(2-3): 1407-1413.
  21. Alaei S.h., Mahna N. Comparison of essential oil composition in Dracocephalum moldavica in green house and field. TEOP 2013;16 (3): 346-351.
  22. Chu S.h., Liu S.h.L., Liu Q. Zh., Liu, Zh. L., Du S.h. Composition and toxicity of Chinese Dracocephalum moldavica (Labiatae) essential oil against two grain storage insects. J. Med. Plants Res. 2011; 5(18): 4621-4626
  23. Murashige T., Skoog F.A. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 1962; 15: 473-497.
  24. Allahverdi-Mamaghani B., Hesamzadeh Hejazi S.M., Mirza M., Movafeghi A. Comparison of Essential Oils Composition Between in-vitro Plantlets and Greenhouse Plants from Various Populations of Dracocephalum kotschyi Boiss. J. Med. Plants and By-prod. 2022; 2: 219-230.
  25. Brithish Pharmacopeia, vol. 2, Appendix XI F, HMSO, London. 1988,p. A138.
  26. Adams R.P. Identification of essential oils by Ion trap Mass Spectroscopy. Academic Press. 2017; San Diego, CA.
  27. Davies N.W. Gas chromatoghraphic retention indices of monoterpenes and sesquiterpenes on methylsilicone and carbowax 20M phases. J. Chromatogr. 1990; 503: 1-24.
  28. Shibomoto T. Retention indices in essential oil analysis, In: Sandra P, and Bicchi C (eds.) Capillary Gas Chromathography in essential oil analysis. Editors New York. Alfred Heuthig Verlag. 1987; pp. 259-274.
  29. Ghasemi Pirbalouti A., Nourafcan H., Solyamani-Babadi E., Variation in chemical composition and antibacterial activity of essential oils from Bakhtiari Savory (Satureja bachtiarica Bunge.). TEOP 2017; 20(2): 474-484.
  30. Karami, A., Bohlooli, A., Essential oil chemical diversity of Ducrosia anethifolia (DC) Boiss accession from Iran. TEOP 2017; 20 (5): 1342-1348.
  31. Bajalan I., Rouzbahani R., Ghasemi Pirbalouti A., Maggi F. Quali-quantitative variation of essential oil from Iranian rosemary (Rosmarinus officinalis L.) accessions according to environmental factors. J. Essential Oil Res. 2017; 1-9.
  32. Otroshy M., Moradi K. Microporpagation of medicinal plant Dracocephalum kotschyi Boiss. Via nodal cutting technique. J. Medicinal Plants Res. 2011; 5: 5967-5972.
  33. Mendes M.D., Figueriredo A.C., Oliveeria M.M., Trindade H. Essential oil production in shoot cultures versus field –grown plants of Thymus caespititius. Plant Cell Tissue Org. Cult. 2013; 113: 341-351.
  34. Arikat N.A., Jawad F.M., Karam N.S., Shibil R.A. Micropropagation and accumulation of essential oil in wild sage (Salvia fruticosa Mill.). Sci Hortic-Amsterdam. 2004; 100: 193-202.
  35. Sudria C., Pinol M.T., Palazon J., Cusido R.M., Vila R., Morales C., Bonfill M., Canigueral S. Influence of plant growth regulators on the growth and essential oil content of cultured Lavandula dentate plantlets. Plant Cell Tissue Organ Cult. 1999; 58: 177-184.
  36. Al-Qudah T.S., Shibil R.A., Alali F.Q. In-vitro propagation and secondary metabolites production in wild germander. In-vitro Cell Dev. B-Plant 2011; 47: 496-505.
  37. Julianijr H.R., Koroch A.R., Juliani H.R., and Trippi V.S. Micropropagation of Lippia Junelliana (Mold.) Tronc. Plant Cell Tissue Organ Cult. 1999; 59: 175-179.
  38. Nogueira J.M.F., Romano A. Essential oils from micropropagated plants of Lavandula viridis. Phytochem. Anal. 2002; 13: 4-7.
  39. Zuzarte M.R., Dinis A.M., Cavaleiro C., Salgueiro L.R., Canhoto J.M. Trichomes, essential oil and in-vitro propagation of Lavandula pedunculata (Lamiaceae). Ind. Crop Prod. 2010; 32: 580-587.
  40. Chebel A.V., Koroch A.R., Juliani H.R., Trippi V.S. Micropropagation of Minthostachys mollis (H. B. K) Grieseb and essential oil composition of clonally propagated plants. In-vitro Cell Dev. B- Plant 1998; 34: 249-251.
  41. Hirata T., Murakami S., Ogihara K., Suga T. Volatile monoterpenoid constituents of the plantlets of Mentha spicata produced by shoot tip culture. Phytochemistry 1990; 29: 493-495.
  42. Fraternale D., Giamperi L., Ricci D., Rocchi M.B.L., Guidi L., Epifano F., Marcotullio M.C. The effect of triacontanol on micropropagation and on secretory system of Thymus mastichina. Plant Cell Tissue Organ Cult. 2003; 74: 87-97.
  43. Makowczyn´ska J., Sliwinska E., Kalemba D., Pia˛tczak E., Wysokin´ska H. In-vitro propagation, DNA content and essential oil composition of Teucrium scorodonia L. ssp. Scorodonia. Plant Cell Tissue Organ Cult. 2016; 127: 1-13.
  44. Andrys D., Kulpa D. In-vitro propagation affects the composition of narrow - leaved lavender essential oil. Acta Chromatographica 2017. doi: 10.1556/1326.2017.00317.
  45. Luczkiewicz M., Jesionek A., Kokotkiewicz A., Migas P., Mardarowicz M., Szreniawa-Sztajnert A., Zabiegala B., Bucinski A. Production of essential oils from in-vitro cultures of Caryopteris species and comparison of their concentrations with in-vivo plants. Acta Physiol. Plant 2015; 37-58.
  46. Skoula M., Abbes J.E., Johnson C.B. Genetic variation of volatiles and rosmarinic acid in populations of Salvia fruticosa Mill growing increte. Biochem. Syst. Ecol. 2000; 28: 551-561.
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