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رویکردی جامع در شناسایی نماتود بیمارگر حشرات Steinernema siamkayai و باکتری همزیستش، Xnoehabdus stockiae با استناد به داده های مولکولی و فنوتیپی | ||
| نامه انجمن حشره شناسی ایران | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 06 شهریور 1403 | ||
| نوع مقاله: مقاله کامل، انگلیسی | ||
| شناسه دیجیتال (DOI): 10.22117/jesi.2024.366133.1628 | ||
| نویسندگان | ||
| لادویی درما؛ کریشناپریا اکرام؛ عاشق حسین بهات* | ||
| گروه علوم زیستی ، دانشگاه چاندیگراه ، موهالی ، پنچاب، هندوستان | ||
| چکیده | ||
| We present the characterization of the entomopathogenic nematode isolate LD_CU, and its associated symbiotic entomopathogenic bacteria. Morphological data of the present strain showed similitude with type population of Steinernema siamkayai, with small morphometric variations. BLASTn analysis of the ITS rRNA sequence of the nematode revealed 100% similarity with S. siamkayai, confirming conspecificity. The pairwise alignment showed no nucleotide differences with the type population of S. siamkayai. Phylogenetic analysis based on ITS rRNA gene sequences supported these findings, placing isolate LD_CU in a monophyletic clade with S. siamkayai, which forms a sister clade with S. huense, S. cumgarense, S. tami, and S. minutum. Biochemical tests identified the associated bacterial symbiont as Xenorhabdus stockiae. The isolate tested negative for oxidase, catalase, nitrate reductase, O-Nitrophenyl-β-D galactopyranoside (ONPG), methyl red, tryptophan deaminase, indole production, ornithine, lysine, citrate, malonate, hydrogen sulfide, acetoin, and phenylalanine deaminase, but positive for urea and esculin hydrolysis, motility, and acid production from glucose fermentation. The bacteria exhibited neutral red adsorption on MacConkey agar and formed brownish pigmented colonies on nutrient agar. Molecular characterization using 16S rRNA gene sequences revealed 100% similarity with X. stockiae and 90% similarity with X. innexi, indicating significant divergence from other Xenorhabdus species. Phylogenetic analysis showed a close relation to X. stockiae VP-2016b and distinct differences from X. innexi DSM 16336T. Pairwise alignment confirmed no nucleotide differences between the present bacterial strain and X. stockiae. This detailed and comprehensive profiling supports the accurate identification of Steinernema siamkayai and its bacterial symbiont, Xenorhabdus stockiae, and contributes valuable information to the taxonomy and phylogeny of these organisms. | ||
| کلیدواژهها | ||
| BLAST؛ ITS rRNA؛ Steinernema siamkayai؛ Xenorhabdus stockiae؛ 16S rRNA | ||
| عنوان مقاله [English] | ||
| Integrated Profiling of Xenorhabdus stockiae and Steinernema siamkayai: Molecular and Phenotypic Perspectives | ||
| نویسندگان [English] | ||
| Ladoi Drema؛ Krishnapriya Okram؛ Aashiq Bhat | ||
| Department of Biosciences, Chandigarh University, Mohali 140413, Punjab, India | ||
| چکیده [English] | ||
| We present the characterization of the entomopathogenic nematode isolate LD_CU, and its associated symbiotic entomopathogenic bacteria. Morphological data of the present strain showed similitude with type population of Steinernema siamkayai, with small morphometric variations. BLASTn analysis of the ITS rRNA sequence of the nematode revealed 100% similarity with S. siamkayai, confirming conspecificity. The pairwise alignment showed no nucleotide differences with the type population of S. siamkayai. Phylogenetic analysis based on ITS rRNA gene sequences supported these findings, placing isolate LD_CU in a monophyletic clade with S. siamkayai, which forms a sister clade with S. huense, S. cumgarense, S. tami, and S. minutum. Biochemical tests identified the associated bacterial symbiont as Xenorhabdus stockiae. The isolate tested negative for oxidase, catalase, nitrate reductase, O-Nitrophenyl-β-D galactopyranoside (ONPG), methyl red, tryptophan deaminase, indole production, ornithine, lysine, citrate, malonate, hydrogen sulfide, acetoin, and phenylalanine deaminase, but positive for urea and esculin hydrolysis, motility, and acid production from glucose fermentation. The bacteria exhibited neutral red adsorption on MacConkey agar and formed brownish pigmented colonies on nutrient agar. Molecular characterization using 16S rRNA gene sequences revealed 100% similarity with X. stockiae and 90% similarity with X. innexi, indicating significant divergence from other Xenorhabdus species. Phylogenetic analysis showed a close relation to X. stockiae VP-2016b and distinct differences from X. innexi DSM 16336T. Pairwise alignment confirmed no nucleotide differences between the present bacterial strain and X. stockiae. This detailed and comprehensive profiling supports the accurate identification of Steinernema siamkayai and its bacterial symbiont, Xenorhabdus stockiae, and contributes valuable information to the taxonomy and phylogeny of these organisms. | ||
| کلیدواژهها [English] | ||
| BLAST, ITS rRNA, Steinernema siamkayai, Xenorhabdus stockiae, 16S rRNA | ||
| مراجع | ||
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