| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 3,021 |
| تعداد مقالات | 33,767 |
| تعداد مشاهده مقاله | 45,085,201 |
| تعداد دریافت فایل اصل مقاله | 50,472,604 |
بهینه سازی کشت درون شیشه ای با بررسی نوع ریزنمونه و شیوه ضدعفونی بر درصد شاخه زایی برخی از ارقام و ژنوتیپ‌های بادام ( (Prunus dulcis Mill) | ||
| علوم و فناوری بذر ایران | ||
| مقاله 6، دوره 13، شماره 3 - شماره پیاپی 35، مهر 1403، صفحه 87-104 اصل مقاله (1000.6 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijsst.2023.360061.1454 | ||
| نویسندگان | ||
| فرزانه رضوی* 1؛ معصومه اسدی آقبلاغی2 | ||
| 1استادیار، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان چهارمحال و بختیاری، سازمان تحقیقات، آموزش و ترویج کشاورزی، شهرکرد، ایران. | ||
| 2دانش‌آموخته دکتری علوم و تکنولوژی بذر، گروه زراعت و اصلاح نباتات، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران. | ||
| چکیده | ||
| استفاده از فن‌آوری کشت بافت برای تکثیر رویشی یا ریزازدیادی درختان میوه از مهم‌ترین کاربردهای تجاری این شیوه در حوزه کشاورزی و باغبانی میباشد. تحقیق حاضر با هدف تعیین بهترین نوع ریز نمونه و شیوه ضدعفونی برای ریز ازدیادی 11 رقم و ژنوتیپ بادام (Prunus dulcis) در سال‌های 8931 تا 0041 اجرا گردید. آزمایش بصورت فاکتوریل سه عاملی شامل ریز نمونه (خشبی یا علفی)، شیوه ضدعفونی با هیپوکلرید سدیم (غلظت 25% ، 15 دقیقه یا غلظت 50% ،10 دقیقه) و ژنوتیپ/ رقم (ارقام مامایی (M)، شکوفه (SH) و فرانیس (F) و هشت ژنوتیپ اصلاحی) در قالب طرح پایه کاملا تصادفی با سه تکرار اجراء شد. شیوه ضدعفونی، ریز نمونه، ژنوتیپ /رقم و اثرات متقابل این عوامل، تأثیر معنی‌داری بر شاخهزایی بادام داشتند. در ژنوتیپ های A1، A3، A8، A9 و ارقام شکوفه، مامایی و فرانیس، ریزنمونه خشبی درصد شاخهزایی بالاتری نسبت به ریز نمونه علفی داشت. در ژنوتیپ A1، ریز نمونه خشبی دارای درصد شاخه زایی بالاتری (20/40 ) نسبت به ریزنمونه علفی (12/30) بوده، در ژنوتیپ A7، ریزنمونه علفی منجر به شاخهزایی بالاتری (13/40) نسبت به ریزنمونه خشبی (20/36) شد. در ژنوتیپ های A1، A2، A3، A4 و رقم شکوفه، هیپوکلرید سدیم با غلظت 25% برای 15 دقیقه باعث کاهش شاخهزایی نسبت به غلظت 50 درصد برای 10 دقیقه شده است. اثر متقابل سه عامل ژنوتیپ/رقم، شیوه ضد عفونی و ریز نمونه دارای تاثیر معنی دار بر میزان شاخهزایی بادام بود. سایر ترکیبات ضدعفونی کننده، ریز نمونه‌ها و محیط کشت‌های مختلف در ریزازدیادی بادام درآینده بررسی خواهند شد. | ||
| کلیدواژه‌ها | ||
| بادام؛ ریزازدیادی؛ کشت بافت؛ پرآوری؛ ضدعفونی | ||
| عنوان مقاله [English] | ||
| The optimization of micro propagation in almond (Prunus dulcis L.) in some genotypes/cultivars: To assess the effects of explant’s type and sterilization’s method on proliferation | ||
| نویسندگان [English] | ||
| Farzaneh Razavi1؛ Masume Asadi Aghbolaghi2 | ||
| 1Assistant Professor, ChaharMahal O Bakhtiari’s Agricultural Research, Education and Extension Organization(AREEO), Shahrekord, Iran. | ||
| 2Ph.D. Graduate in Seed Science and Technology, Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| چکیده [English] | ||
| This study focused to optimize micropropagation in 11 different almond (Prunus dulcis)’s genotypes/cultivars. Main effort was to determine best explant and disinfection’s method in each genotype/cultivar. Almond’s cultivars, Mamaei (M), Shekoufeh (SH) and Fragnies (F) as well as bred almond lines; A1, A2, A3, A4, A5, A7, A8 and A9 were evaluated. The effect of explant; disinfection’s methods and genotype/cultivar were analyzed using factorial experiment on completely randomized design with 3 replications for each treatment. Results indicated 3 studied factors along with their interactions can greatly affect almond’s proliferation. The explant (woody/ herbaceous shoot) showed different effects on proliferation in different almond’s genotypes/cultivars. In genotype A1, herbaceous shoot showed not suitable proliferation (30.12) compared to woody one (40.20), while in genotype A7, herbaceous shoot resulted better (40.13) than woody shoot (36.20). Interestingly, in most studied genotypes/cultivars such as A1, A3, A8, A9, SH, M, and F, woody mature shoot exposed better proliferation. Likewise, sterilization’s method had different effects on proliferation in different genotypes/cultivars. For instance, sodium hypochlorite 25 % /15 (conc. (%)/min.) showed higher proliferation in A7, A8, A9 and F, whereas, in other genotypes/cultivars, using 50 %/10 (conc. (%)/min.) was superior. Results verified almond’s genotype/cultivar and its interaction with explant and disinfection’s method, can significantly affect on proliferation as a genetic related trait. Results indicated almomd’s genotype/cultivar, method of disinfection, type of explant (herbaceous or woody shoot) and their interaction can affect significantly on almond’s proliferation, growth’s performance and rate. | ||
| کلیدواژه‌ها [English] | ||
| Almond, micropropagation, proliferation, MSmedium, sterilization | ||
| مراجع | ||
|
Ainsley, P. J., Collins, G. G., & Sedgley, M. (2000). Adventitious shoot regeneration from leaf explants of almond (Prunus dulcis Mill.). In Vitro Cellular & Developmental Biology, 36(6), 470-474. https://doi.org/10.1007/s11627-000-0084-5 Athenaashari, M., & Zakai Khosroshahi, M. R. (2013). Comprehensive guide to plant tissue culture. Bu Ali Sina University Press. [In Persian] Azazi, S., Mirzaei Nodooshan, H., Imam, M., & Kalateh Jari, S. (2018). Factors affecting the micropropagation of almond (Amygdalus scoparia Spach.). Journal of Plant Research, 31(3), 818-828. [In Persian] Bagheri, H., & Azadi, P. (2002). Plant tissue culture (techniques and experiments). Mashhad University Jihad Publications. [In Persian] Bagheri, S., Amiri, M. A., Davoodi, D., & Entesari, M. (2013). Investigation and comparison of conventional culture containers and periodic bioreactors for mass propagation of GF677 hybrid base (peach × almond). Horticultural Sciences (Agricultural Sciences and Industries), 27(1), 36-43. [In Persian] Chaichi, S., Hassanzadeh, N., Jafarloo, M., & Bai Bordi, A. (2002). Guide to cultivate almonds (planting, holding, harvesting). Publication of Agricultural Education. [In Persian] Channuuntapipat, C., Wirthensohn, M., Ramesh, S. A., Battle, I., Arús, P., Sedgley, M., & Collins, G. (2003). Identification of incompatibility in genotypes of almond (Prunus dulcis) using specific primers based on the introns of the s-alleles. Plant Breeding, 122(2), 164-168. Dagustu, N., Fraser, P., Enfissi, E., & Bramley, P. (2008). Screening for high callus induction and Agrobacterium-mediated transformation of sunflower (Helianthus annuus L.). Biotechnology and Biotechnological Equipment, 22, 933-937. Darab, H., Soleimanpour, R., & Zolfi, A. (2010). Educational set of disinfectants and disinfectants. West Azerbaijan University of Medical Sciences and Health Services. [In Persian] Darvishian, M. (2000). Cultivation and production of almonds.Technical Publishing. [In Persian] Dejmpour, J., Grigorian, A., Majidi, A., & Ansroudi, F. (2010). Evaluation of rooting ability of several interspecific hybrids of Prunus genus in vitro culture. Iranian Journal of Horticultural Science and Technology, 11(1), 1-10. [In Persian] Food and Agriculture Organization of the United Nations (FAO) (2020). Food and agricultural commodities production. http://faostat.fao.org/site/339/default.aspx Fotopoulos, S., & Sotiropoulos, T. E. (2005). In vitro rooting of PR 204/84 rootstock (Prunus persica × P. amygdalus) as influenced by mineral concentration of the culture medium and exposure to darkness for a period. Agronomy Research, 3(1), 3–8. Gerdakaneh, M., Badakhshan, H., Mohamadi, M., & Arji, A. (2020). Effect of different media and growth regulators on micropropagation of GF677. The Plant Production (Scientific Journal of Agriculture), 43(2), 241-254. https://doi.org/10.22055/PPD.2019.27439.1667 Ghasemi, A., Izadi, H., & Masoumifar, H. (2010). Almond cultivation (planting, holding, harvesting). Agricultural Education and Extension Publications. [In Persian] Hartmann, H. T., Kester, D. E., & Davies, F. T. (1990). Plant propagation and practice. Prentice-Hall. Hasandokht, M., & Ebrahimi, R. (2006). Basics of plant tissue culture. Marz Danesh Publications. [In Persian] Izadpanah, M. (2001). Micropropagation of wild cherry (Prunus avium). Pajouhesh-va-sazandegi, 14(3), 6-74. Jalili Marandi, R., & Hakimi Rezaei, J. (2003). Investigating the factors affecting micropropagation of golmohammadi (Rose). Master thesis, Horticulture, Shiraz University. [In Persian] Kamali, K., Majidi, H. A., & Zarghami, R. (2001). Determining the most suitable culture medium and growth conditions for micropropagation of GF677 (peach × almond hybrid). Journal of Seedlings and Seeds, 17(3), 234-243. [In Persian] Kester, D. E., Gradiziel, T. M., & Grassely, C. (1991). Almond (Prunus). Resources of Temperate Fruit and Nut Crops, 290, 701-760. Mam, M., Ghamari Zare, A., Asadi Karam, F., & Loki Anaraki, K. (2013). Micropropagation of almond (Amygdalus scoparia) through bud and embryo culture. Journal of Genetic Research and Breeding of Range and Forest Plants of Iran, 1, 77-86. [In Persian] Miguel, C. M., Druart, F., & Oliveira, M. (1996). Shoot regeneration from adventitious buds induced on juvenile and adult almond (Prunus dulcis Mill.) explants. In Vitro Cellular and Developmental Biology - Plant, 2, 148-153. Mirzaei, F., Durrani, A., & Bandeh Hagh, A. (2017). Effect of genotype, explant and plant growth regulators on intracellular vitiligo (Helianthus annuus L.). Journal of Cellular and Molecular Research, 29(4), 432-424. [In Persian] Moeini, A., & Kahrizi, D. (2003). Plant tissue culture. Tehran: Student Basij Organization Publications. [In Persian] Mohamadzadeh Moghadam, N., & Hamidi, H. (2017). Investigating the effects of medium, sterilization and hormonal treatment on micropropagation of some apple (Mallus domestica Borkh.) rootstocks. The Plant Production (Scientific Journal of Agriculture), 40(1), 41-45. [In Persian] Nazari, A., Grossi, Q., Haddad, R., & Babaei, M. (2010). Study of the effect of pectin, type of culture medium and plant growth regulators on GF677 hybrid base propagation in vitro. Journal of Agricultural Biotechnology, 2(1), 126-113. [In Persian] Nas, M. N., Bolek, Y., & Sevgin, N. (2010). The effects of explant and cytokinin type on regeneration of Prunus microcarpa. Scientia Horticulturae, 126(2), 88-94. https://doi.org/10.1016/j.scienta.2010.06.012 Pierik, R. L. M. (1987). In vitro culture of higher plants. Martinus Nijhoff Publishers. Pierik, R. L. M., Steegmans, H. H. M., & Marelis, J. J. (1973). Gerbera plantlets from in vitro cultivated capitulum explants. Scientia Horticulturae, 1, 107-119. Razavi, F. (2022). The study of physiological and molecular responses to the cold stress in almond (Prunus dulcis Mill.), and cold tolerance degree in different almond genotypes. Final project report, No. 55537, published by AREEO (Agricultural Research, Education & Extension Organization). [In Persian] Rahimi, M. K. (2005). Intensive medical microbiology. Tehran: Ayizh Publications. [In Persian] Rutledge, C. B., & Douglas, G. C. (1988). Culture of meristem tips and micropropagation of 12 commercial clones of poplar in vitro. Physiologia Plantarum, 72, 367-373. Seyed Tabatabai, B. A., & Omidi, M. (2011). Plant tissue and cell culture. University of Tehran. [In Persian] Shahnavazi, A. (2012). Evaluating the economic benefits of Iranian almond research using the economic surplus model. Agricultural Economics Research, 1, 142-121. [In Persian] Shakafandeh, A., & Ghasemi, M. (2008). Investigation of the effects of BA and TDZ on bud growth and body embryogenesis of immature almond cobs (Prunus dulcis L.) late flowering cultivar '7 Shahroud'. Journal of Horticultural Sciences (Agricultural Sciences and Industries), 2, 147-154. [In Persian] Sharifi, A., Moshtaghi, N., & Bagheri, A. (2010). Cultivation of applied plant tissue of some agricultural, horticultural and ornamental products. Mashhad University Jihad. [In Persian] Shtereva, L., Vassilevska-Ivanova, R., Karceva, T., & Kraptchev, B. (2014). Micropropagation of six Paulownia genotypes through tissue culture. Journal of Central European Agriculture, 15, 147-156. Sujatha, M., & Mukta, N. (1996). Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas. Plant Cell, Tissue and Organ Culture, 44, 135-141. Tehranifar, A., Kafi, M., & Adli, M. (2002). Almond cultivation. Tehran: Botany of basic selection and transplantation of agricultural operations of pests and diseases, processing and grading: Mashhad Agricultural Jihad Publications. [In Persian] Wazwai, A. (1999). Investigating the trend of working almonds in Iran and comparing it with several countries in Australia and the United States. Scientific speech, Faculty of Agriculture, Karaj University. [In Persian] Webster, S., Mitchell, S. A., & Ahmad, M. H. (2003). A novel surface sterilization method for reducing microbial contamination of field-grown medicinal explants intended for in vitro culture. Biotechnology Centre of West Indies University 17th SRC Conference on Science and Technology for Economic Development: Technology Driven Agriculture and Agro-Processing, Biotechnology Centre, Mona, Kingston, Jamaica. Xing, W., Bao, M., Qin, H., & Ning, G. (2010). Micropropagation of Rosa rugosa through axillary shoot proliferation. Acta Biologica Cracoviensia Series Botanica, 52, 69-75. Yıldırım, H., Onay, A., Süzerer, V., Tilkat, E., Özden-Tokatlı, Y., & Akdemir, H. (2010). Micrografting of almond (Prunus dulcis Mill.) cultivars "Ferragnes" and "Ferraduel". Scientia Horticulturae, 125, 361–367. Zolfaghari Nasab, R., Khosroshahi, M., Grigorian, V., & Motalebi Azar, A. (2004). Investigation of the increase in natural hybrids of apricot × tomato. Iranian Journal of Horticultural Science and Technology, 2, 81–92. [In Persian]
| ||
|
آمار تعداد مشاهده مقاله: 220 تعداد دریافت فایل اصل مقاله: 146 |
||