اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,043
تعداد مقالات34,036
تعداد مشاهده مقاله46,410,893
تعداد دریافت فایل اصل مقاله77,478,081
Saemi, Fatemeh, shirazi nezhad, Ali, Hosseini, Mohammad Hossein, Hashemi, Majid, Rahimian, Abdollah, Kamgar, Maryam, Rezaeishoorijeh, Reyhaneh, Dabiri, Fatemeh, Hayati, masoumeh, Amiri, sona. (1403). Evaluation of the Efficacy of Humoral Immunity Response of Killed Oil Adjuvant Escherichia coli Vaccine in Layer Chicken against Avian E. coli Serotype O78 Infection. سامانه مدیریت نشریات علمی, 80(1), 161-168. doi: 10.32592/ARI.2025.80.1.161
Fatemeh Saemi; Ali shirazi nezhad; Mohammad Hossein Hosseini; Majid Hashemi; Abdollah Rahimian; Maryam Kamgar; Reyhaneh Rezaeishoorijeh; Fatemeh Dabiri; masoumeh Hayati; sona Amiri. "Evaluation of the Efficacy of Humoral Immunity Response of Killed Oil Adjuvant Escherichia coli Vaccine in Layer Chicken against Avian E. coli Serotype O78 Infection". سامانه مدیریت نشریات علمی, 80, 1, 1403, 161-168. doi: 10.32592/ARI.2025.80.1.161
Saemi, Fatemeh, shirazi nezhad, Ali, Hosseini, Mohammad Hossein, Hashemi, Majid, Rahimian, Abdollah, Kamgar, Maryam, Rezaeishoorijeh, Reyhaneh, Dabiri, Fatemeh, Hayati, masoumeh, Amiri, sona. (1403). 'Evaluation of the Efficacy of Humoral Immunity Response of Killed Oil Adjuvant Escherichia coli Vaccine in Layer Chicken against Avian E. coli Serotype O78 Infection', سامانه مدیریت نشریات علمی, 80(1), pp. 161-168. doi: 10.32592/ARI.2025.80.1.161
Saemi, Fatemeh, shirazi nezhad, Ali, Hosseini, Mohammad Hossein, Hashemi, Majid, Rahimian, Abdollah, Kamgar, Maryam, Rezaeishoorijeh, Reyhaneh, Dabiri, Fatemeh, Hayati, masoumeh, Amiri, sona. Evaluation of the Efficacy of Humoral Immunity Response of Killed Oil Adjuvant Escherichia coli Vaccine in Layer Chicken against Avian E. coli Serotype O78 Infection. سامانه مدیریت نشریات علمی, 1403; 80(1): 161-168. doi: 10.32592/ARI.2025.80.1.161

Evaluation of the Efficacy of Humoral Immunity Response of Killed Oil Adjuvant Escherichia coli Vaccine in Layer Chicken against Avian E. coli Serotype O78 Infection

Archives of Razi Institute
مقاله 19، دوره 80، شماره 1، فروردین و اردیبهشت 2025، صفحه 161-168    اصل مقاله (357.92 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.1.161
نویسندگان
Fatemeh Saemi* 1؛ Ali shirazi nezhad2؛ Mohammad Hossein Hosseini1؛ Majid Hashemi3؛ Abdollah Rahimian1؛ Maryam Kamgar4؛ Reyhaneh Rezaeishoorijeh5؛ Fatemeh Dabiri1؛ masoumeh Hayati1؛ sona Amiri6
1Razi Vaccine and Serum Research Institute, Shiraz Branch, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran
2ریاست موسسه رازی شعبه شیراز
3Shiraz Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran
4Razi institute
5Kazzeron university
6razi institute
چکیده
Colibacillosis is one of the most important bacterial diseases of chickens and turkeys which caused by avian pathogenic Escherichia coli (APEC). Mortality, low body weight, and high FCR in colibacillosis affected poultry farms and higher carcass condemnation at slaughterhouse caused great economic impacts on the poultry industry. In recent years, production of homologous and heterologous APEC vaccines has been evaluated. In this study, mineral oil as an adjuvant for inactivated E. coli used and inoculated via injection route to laying chicken. At 28 days of age, 60 birds were subsequently divided into six experimental groups of 10 chickens per group. Chickens in group control did not receive E. coli vaccines; whereas five treatment groups were vaccinated subcutaneously with a formalin inactivated, mineral-oil adjuvant E. coli vaccine containing isolate of E. coli serotype O78; T1, T2, T3, T4, and T5 groups were vaccinated at 28 days of age with 0.2 ml (8 x106, 16 x106, 33 x106, 66 x106, and 133 x106 cfu/ml) per dose of E. coli O78 respectively. IgG antibody titers against E. coli was evaluated 10 weeks after inoculation with ELISA method. Results showed the significant titer in IgG antibodies in the immunized birds compared to the unimmunized control group (P <0.05), anti IgG antibodies increased weekly after injection of produced compounds in most vaccinated groups up to week four. Overall, prepared E. coli vaccine in Razi institute, Shiraz branch induced high levels of immune responses in the vaccinated laying hens as revealed by ELISA. Although, to considerable immunological stimulus it is suggested that all the chickens in the experimental group receive a booster dose four weeks after the first immunization.
کلیدواژه‌ها
APEC؛ Colibacillosis؛ Immunization؛ Vaccine
مراجع
  1. Rawiwet V, Chansiripornchai N. The efficacy of Escherichia coli aroA-live vaccine in broilers against avian E. coli serotype O78 infection. The Thai Journal of Veterinary Medicine. 2009; 39(4):337-42.
  2. Ghunaim H, Abu-Madi MA, Kariyawasam S. Advances in vaccination against avian pathogenic Escherichia coli respiratory disease: potentials and limitations. Veterinary microbiology. 2014 Aug 6; 172(1-2):13-22.
  3. Lozica L, Morteza Gholi CS, Kela A, Lošić I, Horvatek Tomić D, Gottstein Ž. Autogenous Escherichia coli vaccine application as an innovative antimicrobial therapy in poultry farming—A case report. Vaccines. 2022 Sep 19; 10(9):1567.
  4. Alber A, Stevens MP, Vervelde L. The bird’s immune response to avian pathogenic Escherichia coli. Avian Pathology. 2021 Sep 3;50(5):382-91.
  5. Li L, Thøfner I, Christensen JP, Ronco T, Pedersen K, Olsen RH. Evaluation of the efficacy of an autogenous Escherichia coli vaccine in broiler breeders. Avian Pathology. 2017 May 4; 46(3):300-8.
  6. Ali A, I Abd El-Mawgoud A, M Dahshan AH, A EL-Sawah A, A Nasef S. Escherichia coli in broiler chickens in Egypt, its virulence traits and vaccination as an intervention strategy. Novel Research in Microbiology Journal. 2019 Aug 1; 3(4):415-27.
  7. Filho TF, Fávaro Jr C, Ingberman M, Beirão BC, Inoue A, Gomes L, Caron LF. Effect of spray Escherichia coli vaccine on the immunity of poultry. Avian Diseases. 2013 Sep 1; 57(3):671-6.
  8. Gyles CL. Antimicrobial resistance in selected bacteria from poultry. Animal health research reviews. 2008 Dec;9 (2):149-58.
  9. Cowan ST. Cowan and Steel's manual for the identification of medical bacteria.1974.
  10. DebRoy C, Roberts E, Fratamico PM. Detection of O antigens in Escherichia coli. Animal health research reviews. 2011 Dec;12(2):169-85.
  11. Barnes HJ. Colibacillosis. Diseases of poultry. 2003:631-52.
  12. Lynne AM, Foley SL, Nolan LK. Immune response to recombinant Escherichia coli Iss protein in poultry. Avian Diseases. 2006 Jun 1;50(2):273-6.
  13. Bolin CA, Jensen AE. Passive immunization with antibodies against iron-regulated outer membrane proteins protects turkeys from Escherichia coli septicemia. Infection and immunity. 1987 May; 55(5):1239-42.
  14. Roland K, Karaca K, Sizemore D. Expression of Escherichia coli antigens in Salmonella typhimurium as a vaccine to prevent airsacculitis in chickens. Avian diseases. 2004 Sep 1; 48(3):595-605.
  15. Kariyawasam S, Wilkie BN, Hunter DB, Gyles CL. Systemic and mucosal antibody responses to selected cell surface antigens of avian pathogenic Escherichia coli in experimentally infected chickens. Avian Diseases. 2002 Jul 1; 46(3):668-78.
  16. Śmiałek M, Kowalczyk J, Koncicki A. Influence of vaccination of broiler chickens against Escherichia coli with live attenuated vaccine on general properties of E. coli population, IBV vaccination efficiency, and production parameters-A field experiment. Poultry science. 2020 Nov 1; 99(11):5452-60.
  17. Abd El-Mawgoud AI, El-Nahass ES, Shany SA, El-Sawah AA, Dahshan AH, Nasef SA, Ali A. Efficacy of live attenuated vaccine and commercially available lectin against avian pathogenic E. coli infection in broiler chickens. Veterinary Sciences. 2020 May 13;7(2):65.
  18. Roland K, Curtiss III R, Sizemore D. Construction and evaluation of a Δcya Δcrp Salmonella typhimurium strain expressing avian pathogenic Escherichia coli O78 LPS as a vaccine to prevent airsacculitis in chickens. Avian diseases. 1999 Jul 1:429-41.
  19. Landman WJ, Van Eck JH. The efficacy of inactivated Escherichia coli autogenous vaccines against the E. coli peritonitis syndrome in layers. Avian Pathology. 2017 Nov 2;46(6):658-65.
  20. Christensen H, Bachmeier J, Bisgaard M. New strategies to prevent and control avian pathogenic Escherichia coli (APEC). Avian Pathology. 2021 Sep 3;50(5):370-81.
  21. Gregersen RH, Christensen H, Ewers C, Bisgaard M. Impact of Escherichia coli vaccine on parent stock mortality, first week mortality of broilers and population diversity of E. coli in vaccinated flocks. Avian Pathology. 2010 Aug 1;39(4):287-95.
  22. La Ragione RM, Woodward MJ, Kumar M, Rodenberg J, Fan H, Wales AD, Karaca K. Efficacy of a live attenuated Escherichia coli O78∶ K80 vaccine in chickens and turkeys. Avian diseases. 2013 Jun 1;57(2):273-9.
  23. Koutsianos D, Gantelet H, Franzo G, Lecoupeur M, Thibault E, Cecchinato M, Koutoulis KC. An assessment of the level of protection against colibacillosis conferred by several autogenous and/or commercial vaccination programs in conventional pullets upon experimental challenge. Veterinary Sciences. 2020 Jun 30;7(3):80.
  24. Kariyawasam S, Wilkie BN, Gyles CL. Resistance of broiler chickens to Escherichia coli respiratory tract infection induced by passively transferred egg-yolk antibodies. Veterinary microbiology. 2004 Mar 5;98(3-4):273-84.
آمار
تعداد مشاهده مقاله: 1,069
تعداد دریافت فایل اصل مقاله: 409


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب