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Sanakhan Rezaiyeh, Hadiseh, Modiri, Leila, Chaichi Nosrati, Arash. (1404). Molecular Characterization of nfsA and nfsB Genes in Furazolidone-Resistant Salmonella Spp. Isolated from Poultry Eggs. سامانه مدیریت نشریات علمی, 80(2), 373-382. doi: 10.32592/ARI.2025.80.2.373
Hadiseh Sanakhan Rezaiyeh; Leila Modiri; Arash Chaichi Nosrati. "Molecular Characterization of nfsA and nfsB Genes in Furazolidone-Resistant Salmonella Spp. Isolated from Poultry Eggs". سامانه مدیریت نشریات علمی, 80, 2, 1404, 373-382. doi: 10.32592/ARI.2025.80.2.373
Sanakhan Rezaiyeh, Hadiseh, Modiri, Leila, Chaichi Nosrati, Arash. (1404). 'Molecular Characterization of nfsA and nfsB Genes in Furazolidone-Resistant Salmonella Spp. Isolated from Poultry Eggs', سامانه مدیریت نشریات علمی, 80(2), pp. 373-382. doi: 10.32592/ARI.2025.80.2.373
Sanakhan Rezaiyeh, Hadiseh, Modiri, Leila, Chaichi Nosrati, Arash. Molecular Characterization of nfsA and nfsB Genes in Furazolidone-Resistant Salmonella Spp. Isolated from Poultry Eggs. سامانه مدیریت نشریات علمی, 1404; 80(2): 373-382. doi: 10.32592/ARI.2025.80.2.373

Molecular Characterization of nfsA and nfsB Genes in Furazolidone-Resistant Salmonella Spp. Isolated from Poultry Eggs

Archives of Razi Institute
مقاله 11، دوره 80، شماره 2، خرداد و تیر 2025، صفحه 373-382    اصل مقاله (972.54 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.2.373
نویسندگان
Hadiseh Sanakhan Rezaiyeh؛ Leila Modiri* ؛ Arash Chaichi Nosrati
Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran.
چکیده
Furazolidone (FZD), a broad-spectrum antibiotic in the nitrofuran class, is banned in many countries due to health concerns. The illegal use of FZD in poultry can lead to drug resistance in bacteria, such as Salmonella spp., which infect both poultry and humans. Contaminated eggs are a primary source of Salmonella infection. This study investigated the resistance of Salmonella isolates from eggs to FZD to gain crucial insights into the prevalence of resistant strains within the population. To this end, the susceptibility of 22 Salmonella enterica isolates from eggshells to FZD was determined using the disk diffusion and minimum inhibitory concentration methods. Then, the mutations in the nfsA and nfsB genes were examined using the polymerase chain reaction method and sequencing. Results were analyzed using GeneRunner software and BLAST online software. It was found that 27.27% and 9.09% of the isolates had high and medium resistance to FZD, respectively. The minimum inhibitory concentration results were determined to be 32 μg/ml for sensitive isolates, 256 μg/ml for intermediate isolates, and 512 μg/ml for resistant isolates. Sequencing analysis identified six insertion mutations and one transition mutation in the nfsA gene of resistant isolates, as well as one silent mutation in the nfsB gene of a sensitive isolate. The study highlights substantial resistance to FZD in Salmonella isolates from eggs, associated with mutations in the nfsA gene. These findings underscore the necessity for monitoring and managing resistance in foodborne pathogens. The significant resistance to FZD and the related mutations in the nfsA gene highlight the critical need for continuous surveillance and research to address the growing issue of antimicrobial resistance, especially in food products.
کلیدواژه‌ها
Furazolidone؛ nfsA؛ nfsB؛ Resistance؛ Salmonella
عنوان مقاله [English]
بررسی خصوصیات مولکولی ژن‌های nfsA و nfsB در ایزوله های سالمونلا مقاوم به فورازولیدون جدا شده از تخم مرغ
چکیده [English]
فرازولیدون (FZD)، یک آنتی‌بیوتیک وسیع‌الطیف از دسته نیتروفوران‌ها، به دلیل نگرانی‌های بهداشتی در بسیاری از کشورها ممنوع شده است. استفاده غیرقانونی از FZD در طیور می‌تواند به بروز مقاومت دارویی در باکتری‌ها، از جمله گونه های سالمونلا، که هم بر روی طیور و هم بر روی انسان‌ها تأثیر می‌گذارند، منجر شود. تخم‌مرغ‌های آلوده منبع اصلی عفونت باکتری سالمونلا هستند. این مطالعه به بررسی مقاومت ایزوله‌های سالمونلا از تخم‌مرغ‌ها به FZD پرداخته است تا اطلاعات مهمی درباره شیوع سویه‌های مقاوم در جمعیت به دست آورد. به این منظور، حساسیت 22 ایزوله سالمونلا انتریکا از پوسته‌های تخم‌مرغ به FZD با استفاده از روش‌های انتشار دیسک و حداقل غلظت مهار کنندگی(MIC) تعیین شد. سپس جهش‌های ژن‌های nfsA و nfsB با روش واکنش زنجیره‌ای پلیمراز و تعیین توالی مورد بررسی قرار گرفت و نتایج آن با استفاده از نرم‌افزار GeneRunner و نرم‌افزار آنلاین BLAST مورد تجزیه و تحلیل قرار گرفت. 27.27 و 9.09 درصد جدایه ها به ترتیب مقاومت بالا و متوسط به فورازولیدون داشتند. نتایج MIC برای جدایه های حساس، متوسط و مقاوم به ترتیب 32، 256 و 512 میکروگرم بر میلی لیتر تعیین شد. تجزیه و تحلیل نتایج توالی‌یابی شش جهش درج و یک جهش انتقالی در nfsA در ایزوله‌های مقاوم و با مقاومت متوسط و یک جهش بی‌صدا در nfsB در یک ایزوله حساس نشان داد.این مطالعه مقاومت قابل توجهی به FZD در ایزوله‌های سالمونلا از تخم‌مرغ‌ها را نشان می‌دهد که با جهش‌های ژن nfsA مرتبط است. این یافته‌ها بر ضرورت پایش و مدیریت مقاومت در پاتوژن‌های غذایی تأکید می‌کند. مقاومت قابل توجه به FZD و جهش‌های مرتبط در ژن nfsA نیاز حیاتی به پایش و تحقیق مستمر برای مقابله با مسئله روزافزون مقاومت میکروبی، به ویژه در محصولات غذایی، را برجسته می‌کند.
کلیدواژه‌ها [English]
فورازولیدون, nfsA, nfsB, مقاومت, سالمونلا
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