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Comparative study of Amatem and ethanol leaf extract of Mangifera indica on male reproductive hormones and histology of the testis | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 02 فروردین 1404 اصل مقاله (627.25 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2025.367693.3420 | ||
| نویسندگان | ||
| Justina Nwandimma Nwangwa1؛ Ekementeabasi Aniebo Umoh* 2؛ ESU UKPAI ENENE3 | ||
| 1Department of Physiology, University of Calabar, Calabar, Cross River State, Nigeria. | ||
| 2Department of Physiology, Arthur Jarvis University, Akpabuyo, Cross River State, Nigeria | ||
| 3Department of Physiology, Federal University Wukari, Wukari, Taraba State, Nigeria. | ||
| چکیده | ||
| Conflicting reports on reproductive disorders following the use of antimalarials have been recorded. This research aimed at comparing an orthodox drug (Amatem) with a herbal remedy (Mangifera indica) used for treating malaria on male reproductive hormone with the view of ascertaining which will be protective on reproductive parameters. Twenty-five male rats weighing 250-300 g were used for this study. Animals were divided into five groups, namely: Control, low dose M. indica (Mag [LD]), middle dose M. inidca (Mag [MD]), high dose M indica (Mag [HD]), and Amatem group. All animals were allowed access to normal rat chow and water throughout the period of the experiment. Following the oral toxicity study, Mag [LD], Mag [MD], and Mag [HD] were administered ethanolic extract of M. indica leaf at 400 mg/kg, 800 mg/kg, and 1600 mg/kg body weight. Amatem was dissolved in normal saline and administered following the recommended dose of 8 mg/kg body weight. All administration lasted for one week, and sacrifice was carried out on the eighth day. Blood was gotten via cardiac puncture and spun in a centrifuge to get the serum, which was used for testosterone, FSH, and LH assays through the method of enzyme-linked immunoassay (ELISA). The testis was excised and histology done using H&E staining. ANOVA was used to test the level of significance of results among experimental groups. Serum testosterone was higher in Amatem, Mag (MD), and Mag (HD) compared to the control at p < 0.05. There was also a significant increase in testosterone of Amatem compared to Mag (MD) and Mag (HD) at p < 0.05. Follicle stimulating hormone (FSH) and luteinizing hormone (LH) of Amatem, Mag [LD], Mag [MD], and Mag [HD] showed no significant difference with that of the control group. Mild to severe distortions were seen in the histology of the testis in the extract and drug-administered groups. Though Amatem and M. indica increase testosterone levels, caution is needed, as the distortions of the testis may pose harmful effects in the long run. Gas chromatography mass spectrometry (GC-MS) technique revealed 43 compounds, with heptadecane having the most peak areas of 8.00%. | ||
| کلیدواژه‌ها | ||
| Malaria؛ Mango leaf extract؛ Antimalarial | ||
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آمار تعداد مشاهده مقاله: 43 تعداد دریافت فایل اصل مقاله: 85 |
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