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H, CharuMeena, R, Sagaya Jansi, S, Aishwarya, Shanmugaraj, Balamurugan, Panthagani, Praveen Kumar. (1404). Exploring Breast Cancer-Associated Genes: A Comprehensive Analysis and Competitive Endogenous RNA Network Construction. سامانه مدیریت نشریات علمی, 80(2), 447-460. doi: 10.32592/ARI.2025.80.2.447
CharuMeena H; Sagaya Jansi R; Aishwarya S; Balamurugan Shanmugaraj; Praveen Kumar Panthagani. "Exploring Breast Cancer-Associated Genes: A Comprehensive Analysis and Competitive Endogenous RNA Network Construction". سامانه مدیریت نشریات علمی, 80, 2, 1404, 447-460. doi: 10.32592/ARI.2025.80.2.447
H, CharuMeena, R, Sagaya Jansi, S, Aishwarya, Shanmugaraj, Balamurugan, Panthagani, Praveen Kumar. (1404). 'Exploring Breast Cancer-Associated Genes: A Comprehensive Analysis and Competitive Endogenous RNA Network Construction', سامانه مدیریت نشریات علمی, 80(2), pp. 447-460. doi: 10.32592/ARI.2025.80.2.447
H, CharuMeena, R, Sagaya Jansi, S, Aishwarya, Shanmugaraj, Balamurugan, Panthagani, Praveen Kumar. Exploring Breast Cancer-Associated Genes: A Comprehensive Analysis and Competitive Endogenous RNA Network Construction. سامانه مدیریت نشریات علمی, 1404; 80(2): 447-460. doi: 10.32592/ARI.2025.80.2.447

Exploring Breast Cancer-Associated Genes: A Comprehensive Analysis and Competitive Endogenous RNA Network Construction

Archives of Razi Institute
مقاله 9، دوره 80، شماره 2، خرداد و تیر 2025، صفحه 447-460    اصل مقاله (697.07 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.2.447
نویسندگان
CharuMeena H1؛ Sagaya Jansi R* 2؛ Aishwarya S3؛ Balamurugan Shanmugaraj4؛ Praveen Kumar Panthagani5
1Department of Bioinformatics Stella Maris College, Chennai, Tamil Nadu, India
2Department of Bioinformatics, Stella Maris College, Chennai, Tamil Nadu, India
3Department of Bioinformatics, Stella Maris College, Chennai, Tamil Nadu, India
4Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore, 641021, Tamil Nadu, India
5Molecular Biology Department, Ampath Lab, Hyderabad, Telangana
چکیده
Breast cancer is a cancer affecting women in which cells become abnormal and multiplies in an uncontrollable fashion. These cancers are hereditary and gene mutations with geographic indications stand alone in most invasive breast cancer types, several other factors like age, gender, ethnic background and environment also contribute for the occurrence. Non-coding RNAs are competing endogenous RNAs that participate in the progression of different cancers. The present study intends to uncover the differentially expression genes in breast cancer. In this study, with the Breast cancer RNA-Seq data obtained from TCGA, expression and survival analyses were performed in order to study gene expression of the samples and genes computationally through R programming. The results obtained after each analysis were inferred visually and logically. Total 613 genes were found to be differentially expressed among the samples out of which 254 were up regulated and 359 were down regulated. The differentially expressed genes obtained using the package TCGA bio links was further used in the construction of ceRNA network. From the results of analyses using TCGA bio links, 352 lncRNAs, 183 miRNAs and 254 mRNAs were found to show aberrant expression in the breast cancer samples. Out of the 352 lncRNAs, the 2 most commonly and repeatedly expressed lncRNAs LINC00 461 and MALAT 1 were found to be more potent therapeutic biomarker and found that their regulated miRNA target genes are enriched in the samples. This study constructed the competing endogenous RNA networks and furthermore unravels the underlying biomarkers for breast cancer cohorts.
کلیدواژه‌ها
Breast cancer؛ miRNAs؛ lncRNAs؛ ceRNA network؛ R program
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