اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها2,944
تعداد مقالات33,229
تعداد مشاهده مقاله43,866,041
تعداد دریافت فایل اصل مقاله20,372,307
Dare, Babatunde, Blessing, Kolawole, Peter, Peter, Cinderela, Ebele, Olawale, Obembe,, Samson, Adeleke. (1404). Testicular Histological Changes and Ki-67 Expression in Lead (II) Oxide Toxicity among Neonatal Wistar Rats and Modulatory Role of Retinol. سامانه مدیریت نشریات علمی, 80(2), 461-471. doi: 10.32592/ARI.2025.80.2.461
Babatunde Joseph Dare; Kolawole Odunola Blessing; Peter Alabi Peter; Ebele Cinderela; Obembe, O Olawale; Adeleke o Samson. "Testicular Histological Changes and Ki-67 Expression in Lead (II) Oxide Toxicity among Neonatal Wistar Rats and Modulatory Role of Retinol". سامانه مدیریت نشریات علمی, 80, 2, 1404, 461-471. doi: 10.32592/ARI.2025.80.2.461
Dare, Babatunde, Blessing, Kolawole, Peter, Peter, Cinderela, Ebele, Olawale, Obembe,, Samson, Adeleke. (1404). 'Testicular Histological Changes and Ki-67 Expression in Lead (II) Oxide Toxicity among Neonatal Wistar Rats and Modulatory Role of Retinol', سامانه مدیریت نشریات علمی, 80(2), pp. 461-471. doi: 10.32592/ARI.2025.80.2.461
Dare, Babatunde, Blessing, Kolawole, Peter, Peter, Cinderela, Ebele, Olawale, Obembe,, Samson, Adeleke. Testicular Histological Changes and Ki-67 Expression in Lead (II) Oxide Toxicity among Neonatal Wistar Rats and Modulatory Role of Retinol. سامانه مدیریت نشریات علمی, 1404; 80(2): 461-471. doi: 10.32592/ARI.2025.80.2.461

Testicular Histological Changes and Ki-67 Expression in Lead (II) Oxide Toxicity among Neonatal Wistar Rats and Modulatory Role of Retinol

Archives of Razi Institute
مقاله 11، دوره 80، شماره 2، خرداد و تیر 2025، صفحه 461-471    اصل مقاله (659.35 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2025.80.2.461
نویسندگان
Babatunde Joseph Dare* 1؛ Kolawole Odunola Blessing2؛ Peter Alabi Peter3؛ Ebele Cinderela4؛ Obembe, O Olawale5؛ Adeleke o Samson6
1Department of Anatomy, Faculty of Basic Medical Sciences, University of Osun, Osun State, Nigeria
2Department of Anatomy, Osun State University, Osogbo, Nigeria
3Microbiology, Osun State University
4Medical Laboratory, Osun State University
5Department of Physiology, Osun State University, Osogbo, Nigeria
6Department of Anatomy, Health Sciences, Ilorin, Nigeria
چکیده
Lead toxicity has been recognized in soft tissues, whereas retinoid facilitates embryonic development during the intrauterine phase and support tissue regeneration. This study investigated histological changes associated with the testes of animals exposed to lead II oxide during intrauterine period and the protective role of retinol.
Twenty (20) gestational animals were utilized for the study; these animals were subjected to exposure of lead (II) oxide throughout duration of three (3) weeks corresponding to their gestational period. The subjects received daily administration of 60 mg/kg/body weight of lead (II) oxide alongside 25,000 IU/kg body weight of retinol, respectively. Offspring from each designated group were procured post-parturition and subsequently allocated into distinct cages for duration of three (3) weeks comprising a neonatal period of 21 days. The progeny euthanized via cervical dislocation twelve (12) hours subsequent to the completion of the neonatal period of 21 days, followed by the excision of testes, which were then fixed in Bouin’s fluid for histopathological examination and immunohistochemical analysis of Ki-67 protein expression.
The study established teratogenic effect of lead (II) oxide on the testes, degenerative alteration in the seminiferous tubules, vascular congestions and alteration in spermatogonia lineage across the seminiferous tubules. Widening of interstitial spaces and degeneration of leydig cells characterized the animals exposed to intra-uterine lead (II) oxide. Retinol treated expressed improved testicular histological features and expression of Ki-67 migration moiety in neonate testes.
The embryogenic influence of retinol was established through the examination of testicular histological features in animals subjected to intrauterine exposure and the corresponding testicular expression of Ki-67. Consequently, retinol demonstrated protective and fertility-enhancing properties against the detrimental effects induced by exposure to lead (II) oxide
کلیدواژه‌ها
Lead (II) oxide؛ Retinol؛ Infertility؛ Teratogens؛ Wistar Rats
مراجع
  1. RÍsovÁ V. The pathway of lead through the mother's body to the child. Interdisciplinary toxicology. 2020; 12(1), 1–6.
  2. Dare Joseph Babatunde, Olaniyan Olugbemi Tope, Oigiagbe Eboselume Fidelis, Okotie Gloria Enevwo, Eweoya Olugbenga. Aqueous Extract of Adansonia digitata reversed Cotton Seed oil Induced Testicular Damage in Wistar rats. JBRA Assisted Reproduction. 2021; 25(2):257-265.
  3. Sallmen M, Lindbohm ML, Nurminen M. Paternal exposure to lead and infertility. Epidemiology. 2000; 11:148-152.
  4. Naha N., Bhar R.B., Mukherjee A., and Chowdhury A.R. Structural alteration of spermatozoa in the persons employed in lead acid battery factory. Indian J Physiol Pharmacol. 2005;49: 153-162.
  5. Batra, N., Nehru, B., Bansal, M.P. Influence of lead and zinc on rat male reproduction at biochemical and histopathological levels. J. Appl. Toxicol. 2021;21, 507-112.
  6. Zasada M, Budzisz E. Retinoids: active molecules influencing skin structure formation in cosmetic and dermatological treatments. Postepydermatologii i alergologii. 2019;36(4), 392–397.
  7. Khalil S, Bardawil T, Stephan C. Retinoids: a journey from the molecular structures and mechanisms of action to clinical uses in dermatology and adverse effects. J Dermatolog Treat. 2017; 28:684–96.
  8. Duester G. Retinoic acid synthesis and signalling during early organogenesis. Cell. 2008; 134:921–31.
  9. Zizola CF, Frey SK, Jitngarmkusol S. Cellular retinol-binding protein type I (CRBP-I) regulates adipogenesis. Mol Cell Biol. 2010; 30:3412–20.
 

  1. Phang CH. Mating of Rats. In: Murphy, D., Carter, D.A. (eds) Transgenesis Techniques. Methods in Molecular Biology™, 19193; 18.
  2. Offor SJ, Mbagwu HOC and Orisakwe OE. Lead Induced Hepato-renal Damage in Male Albino Rats and Effects of Activated Charcoal. Front. Pharmacol. 2017;8:107.
  3. Dare BJ, Obielodan OA, Adegoke AA, Lawal IA, Adekoya AO. The Nephrotoxicity of Cotton Seed Oil and Gossypol and the Possible Modulatory Role of Grape-seed Proanthocyanidin in Adult Rats Journal of Morphological Sciences. 2023;40,200–207.
  4. Dare J. Babatunde, Doshiya Joseph, Lawal I. Adetayo, Ibiyemi T. Victoria, Abayomi Taiwo. Metabolic activities and cytokeratin-7 (CK7) expression in gossypol induced hepatotoxicity treated with aqueous extract of Phoenix dactyliferain rat model. Eur. J. Anat. 2021;25 (3): 339-349.
  5. Matson CK, Murphy MW, Griswold MD, Yoshida S, Bardwell VJ, Zarkower D. The mammalian doublesex homolog DMRT1 is a transcriptional gatekeeper that controls the mitosis versus meiosis decision in male germ cells. Dev. Cell. 2010; 19, 612–624.
  6. Gautam AK, Agarwal K, Shah BA, Kumar S, Saiyed HN. Lead induced sperm toxicity in mouse and MPG treatment. J. Environ. Biol. 2001; 22, 287-291.
  7. Livera G, Rouiller-Fabre V, Pairault C, Levacher C, Habert R. Regulation and perturbation of testicular functions by vitamin A. Reproduction. 2002;124(2):173-80.
  8. Chowdhury AR. Recent advances in heavy metals induced effect on male reproductive function – A retrospective, Al Ameen J. Med. Sci. 2009; 2:37–42.
  9. Wang C, Zhang Y, Liang J., Shan G, Wang Y, Shi Q. Impacts of ascorbic acid and thiamine supplementation at different concentrations on lead toxicity in testis. ClinicaChimica Acta. 2006; 370, 82-88.
  10. Queiroz EK, Waissmann W. Occupational exposure and effects on the male reproductive system. Cad Saude Publica. 2006; 22:485-493.
  11. Snyder EM, Small C, Griswold MD. Retinoic acid availability drives the asynchronous initiation of spermatogonial differentiation in the mouse. Biol. Reprod. 2010; 83, 783–790.
  12. Helsel A, Griswold MD. Retinoic acid signaling and the cycle of the seminiferous epithelium. CurrOpinEndocrMetab Res. 2019;6:1-6.
  13. Endo T, Freinkman E, de Rooij DG, Page DC. Periodic production of retinoic acid by meiotic and somatic cells coordinates four transitions in mouse spermatogenesis. Proceedings of the National Academy of Sciences of the United States of America. 2017; 114(47):E10132–E10141.
  14. Teletin M, Vernet N, Yu J, Klopfenstein M, Jones JW, Feret B, Kane MA, Ghyselinck NB, Mark M. Two functionally redundant sources of retinoic acid secure spermatogonia differentiation in the seminiferous epithelium. Development (Cambridge, England). 2019;146(1):dev170225.
  15. llah S, Ennab W, Wei Q, Wang C, Quddus A, Mustafa S, et al. Impact of Cadmium and Lead Exposure on Camel Testicular Function: Environmental Contamination and Reproductive Health. Animals. 2023;13(14):2302.
  16. Xiong L, Zhou B, Young JL, Wintergerst K, Cai L. Exposure to low-dose cadmium induces testicular ferroptosis. Ecotoxicol. Environ. Saf. 2022;234:113373.
  17. Ullah S, Ennab W, Wei Q, Wang C, Quddus A, Mustafa S, et al. Male reproductive toxicity of lead in animals and humans. ASCLEPIOS Study Group. Occup Environ Med. 1998; 55(6):364-74.
  18. Sokol Z, Berman N. The effect of age of exposure on lead-induced testicular toxicity, Toxicology. 1991;69(3):269-78.
آمار
تعداد مشاهده مقاله: 39
تعداد دریافت فایل اصل مقاله: 84


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب