Evaluation the effect of binary mixing interactions of triflusulfuron-methyl, chloridazone, and clopyralid on common purslane (Portulaca oleracea L.) by isobole models

AZIMI NAIBI, S.; Aliverdi, A.; AHMADVAND, G.; CHITBAND, A.A.

doi:10.22092/jaep.2025.368333.1538

	Evaluation the effect of binary mixing interactions of triflusulfuron-methyl, chloridazone, and clopyralid on common purslane (Portulaca oleracea L.) by isobole models
آفات و بیماری‌ های گیاهی
Volume 92, Issue 2, March 2025, Pages 280-293 PDF (930.01 K)
Document Type: Weed Science
DOI: 10.22092/jaep.2025.368333.1538
Authors
S. AZIMI NAIBI¹; A. Aliverdi^* ²; G. AHMADVAND²; A.A. CHITBAND³
¹MSc student in Agrotechnology - Weed Science, Department of Plant Production and Genetics, Faculty of Agriculture, Bu-Ali Sina University, Hamedan
²Associate Professor, Department of Plant Production and Genetics, Faculty of Agriculture, Bu-Ali Sina University, Hamedan
³Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
Abstract
To investigate effect of mixing triflusulfuron-methyl, chloridazone, and clopyralid on common purslane, three experiments were separately conducted at outdoor condition at Bu-Ali Sina University, Hamadan in the summer of 2023. In each experiment, 0, 6.25, 12.5, 25, 50, and 100% of the labeled dose of the two herbicides were prepared in the mixture ratios of (100:0), (80:20), (60:40), (40:60), (20:80), and (0:100) and applied at the 4-5 leaf stage of common purslane. The mixability of the herbicides was evaluated using an appropriate isobole model, after fitting a suitable isobole model (based on a lack-of-fit test), on the ED₅₀ values (herbicide rate required to control 50% of common purslane) obtained from different mixture ratios. The good fit of the linear Additive Dose model on the ED₅₀ values obtained from different mixture ratios of chloridazone + triflusulfuron-methyl indicates an additive effect between them; therefore, they are mixable. The good fit of the nonlinear Hewlett model on the ED₅₀ values obtained from different mixture ratios of clopyralid + triflusulfuron-methyl (λ = 1.66) indicates a synergistic effect between them; therefore, they are also mixable. While the good fit of the nonlinear Woelund model on the ED₅₀ values obtained from different mixture ratios of clopyralid + chloridazone (η₁ = 1.7 and η₂ = 3.8) indicates an antagonistic interaction between them, thus they are not mixable.
Keywords
Additive effect; antagonistic effect; Hewlett model; synergistic effect; Voelund model

References
ABBASPOOR, M., A.A. CHITBAND, M.R. MOLKARA and H. TAVAKOLI. 2013. Using isobolographic analysis for the evaluation of additive, synergism and antagonism effects in binary mixture of glyphosate and clopyralid on Acroptylon repense control. Iranian Plant Protection Research. 27: 294-300. DOI: http://doi.org/10.22067/jpp.v27i3.26731 ARMEL, G.R., P.L. RARDON, M.C. MCCOMRICK and N.M. FERRY, 2007. Differential response of several carotenoid biosynthesis inhibitors in mixtures with atrazine. Weed Technology. 21: 947-53. DOI: http://doi.org/10.1614/wt-06-133.1 ASHIGH, J. and J.C. HALL, 2010. Bases for interactions between saflufenacil and glyphosate in plants. Journal of Agricultural and Food Chemistry. 58: 7335-7343. DOI: http://doi.org/10.1021/jf100595a BARBIERI, G.F., B.G. YOUNG, F.E. DAYAN, J.C. STREIBIG, H.TAKANO, Jr.A. MEROTTO and L.A. AVILA, 2022. Herbicide mixtures: interactions and modeling. Advances in Weed Science. 40: e020220051. DOI: http://doi.org/ 10.51694/AdvWeedSci/2022;40:seventy-five011 BERENBAUM, M.C., 1981. Criteria for analyzing interactions between biologically active agents. Advances in Cancer Research. 35: 269-335. DOI: http://doi.org/10.1016/S0065-230X(08)60912-4 BESANÇON, T.E., D. PENNER and W.J. EVERMAN, 2018. Reduced translocation is associated with antagonism of glyphosate by glufosinate in giant foxtail (Setaria faberi) and velvetleaf (Abutilon theophrasti). Weed Science. 66: 159-167. DOI: 10.1017/wsc.2017.72 BLACKSHAW R.E. 1989. Synergistic mixes of DPX-A7881 and clopyralid in canola (Brassica napus). Weed Technology. 3: 690-695. DOI: http://doi.org/10.1017/s0890037x00033054 BUSI, R., T.A. GAINES and S. POWLES, 2017. Phorate can reverse P450 metabolism-based herbicide resistance in Lolium rigidum. Pest Management Science. 73: 410-417. DOI: http://doi.org/10.1002/ps.4441 CHITBAND, A.A., R. GHORBANI, M.H. RASHED MOHASSEL and M. NABIZADE. 2018. Joint action of some usable important broadleaf herbicides in sugar beet. Iranian Plant Protection Research. 31: 374-387. DOI: http://doi.org/10.22067/jpp.v31i3.50875 CHITBAND, A.A., R. GHORBANI, M. NABIZADE and E. ZAIDALI, 2017. Efficacy of mixing common herbicides in control of broadleaf weeds in sugar beet (Beta vulgaris L.). Journal of Sugar Beet. 33: 91-102. DOI: http://doi.org/10.22092/jsb.2017.101878.1099 CHITBAND, A.A., V. SARABI and A. ALIVERDI, 2025. Joint action of common herbicides on the control of Alhagi pseudalhagi (Bieb.) Desv.: A comparison study. Weed Research. 65: e12679. DOI: http://doi.org/10.1111/wre.12679 DEVEIKYTE, I., L. SARUNAITE and V. SEIBUTIS, 2015. Evaluation of pre- and postemergence herbicide combinations for broadleaved weeds in sugar beet. In: PRICE, A., J. KELTON and L. SARUNAITE. Herbicides, agronomic crops and weed biology. IntechOpen. DOI: http://doi.org/10.5772/61437 FAO. 2023. Food and Agriculture Organization www.fao.org/faostat/en/#data/QC FINCH, B., H. WRIGHT-SMITH, J. DAVIS, AND B. SCOTT, 2024. Jar Test Instructions: Assessing Pesticide-Fertilizer Compatibility (Jar Test). University of Arkansas. FSA2166R. www.uaex.uada.edu/publications/PDF/FSA-2166.pdf HEAP I, 2025. The international herbicide-resistant weed database. www.weedscience.org HICKS, T.V., G.R. WEHTJE and T.L. GREY, 1998. The interaction of pyridate and 2,4-DB in peanut (Arachis hypogaea), Florida beggarweed (Desmodium tortuosum), and sicklepod (Senna obtusifolia). Weed Science. 46: 284-288. DOI: http://doi.org/10.1017/s0043174500089426 HOLLAWAY, K.L., N.D. HALLAM and A.G. FLYNN, 1996. Synergistic joint action of MCPA ester and metsulfuron-methyl. Weed Research. 36: 369-374. DOI: http://doi.org/ 10.1111/j.1365-3180.1996.tb01666.x HOSSEYNI, S.M., H. NAJAFI, B. SANI and H. MOZAFARI, 2022. Investigation the effect of the application of single and mixing of propyzamide and ethofumesate herbicides in the control of dodder (Cuscuta campestris), grass and broad leaves weeds of sugar beet (Beta vulgaris L.). Journal of Crop Ecophysiology. 16: 563-580. DOI: http://doi.org/10.30495/JCEP.2023.1932247.1806 (in Persian with English summary) JING, X., G. YAO, D. LIU, M. LIU, P. WANG and Z. ZHOU, 2016. Environmental fate of chiral herbicide fenoxaprop-ethyl in water-sediment microcosms. Scientific Reports. 6: 26797. DOI: http://doi.org/10.1038/srep26797 KELLEY, K.B., L.M. WAX, A.G. HAGER and D.E. RIECHERS, 2005. Soybean response to plant growth regulator herbicides is affected by other postemergence herbicides. Weed Science. 53: 101-112. DOI: http://doi.org/10.1614/ws-04-078r LE, V.V., A.V. NGUYEN, D.T. LUU, F.B. FRITSCHI, C.T. NGUYEN, T.L. HO, 2024. Inhibitory effects of N-trans-cinnamoyltyramine on growth of invasive weeds and weedy rice. Plant-Environment Interactions. 5: e70017. DOI: 10.1002/pei3.70017 MAJIDI, M., G. HEIDARI and Y. EMAM, 2023. Qualitative characteristics of sugar beet as affected by different broadleaf herbicides combinations. Iran Agricultural Research. 36: 1-6. DOI: http://doi.org/10.22099/iar.2017.4126 (in Persian with English summary) MASABNI, J.G. and B.H. ZANDSTRA, 1999. Discovery of a common purslane (Portulaca oleracea) biotype resistant to linuron. Weed Technology. 13: 599-605. DOI: http://doi.org/10.1017/S0890037X00046261 OTTIS, B.V., J.D. MATTICE and R.E. TALBERT, 2005. Determination of antagonism between cyhalofop-butyl and other rice (Oryza sativa) herbicides in barnyardgrass (Echinochloa crus-galli). Journal of Agricultural and Food Chemistry. 53: 4064-068. DOI: http://doi.org/10.1021/ jf050006d OU, J., C.R. THOMPSON, P.W. STAHLMAN, N. BLOEDOW and M. JUGULAM, 2018. Reduced translocation of glyphosate and dicamba in combination contributes to poor control of Kochia scoparia: evidence of herbicide antagonism. Scientific Reports. 8: 1-11. DOI: http://doi.org/10.1038/ s41598-018-23742-3 PARADOWSKI, A. and T. PRACZYK, 2004. Evaluation of selected mixtures of active ingredients for weed control in sugar beet. Progress in Plant Protection. 44: 1004-1007. DOI: http://doi.org/10.24425/jppr.2023.146878 RASHIDI, S., A.R. YOUSEFI, N. GOICOECHEA, M. POURYOUSEF, P. MORADI, S. VITALINI and M. IRITI, 2021. Allelopathic interactions between seeds of Portulaca oleracea L. and crop species. Applied Sciences. 11: 3539. DOI: http://doi.org/10.3390/app11083539 RITZ, C., F. BATY, J.C. STREIBIG and D. GERHARD, 2015. Dose-response analysis using R. PLoS One. 10: e0146021. DOI: http://doi.org/10.1371/journal.pone.0146021 SCHUSTER, C.L., K. AL-KHATIB and J.A. DILLE, 2007. Mechanism of antagonism of mesotrione on sulfonylurea herbicides. Weed Science. 55: 429-434. DOI: 10.1614/ws-06-217.1 SØBYE, K.W., J.C. STREIBIG, N. CEDERGREEN, 2011. Prediction of joint herbicide action by biomass and chlorophyll a fluorescence. Weed Research. 5:23-32. DOI: http://doi.org/10.1111/j.1365-3180.2010.00824.x SOROKA, S.V. and G.J. GADZHIEVA, 2006. State of weed infestation and features of sugar beet protection in Belarus. Matica Srpska Journal for Natural Sciences. 110: 165-172. DOI: http://doi.org/10.2298/ZMSPN0610165S TEYMOURINIA, M., A.A. CHITBAND, G. REZAEE and S. KHAYRANDISH, 2023. The joint action of glyphosate, clethodim, and imazethapyr to control cogongrass (Imperata cylinderica L. Beauv) in the margin of the irrigation canals (A case study using two different approaches). Crop Protection. 174: 106413. DOI: http://doi.org/10.1016/j.cropro.2023.106413 WANAMARTA, G., D. PENNER and J.J. KELLS, 1989. The basis of bentazon antagonism on sethoxydim absorption and activity. Weed Science. 37: 400-404. DOI: http://doi.org/10.1017/s004317450007212x WILSON R.G., 1994. New herbicides for postemergence application in sugarbeet (Beta vulgaris). Weed Technology. 8: 807-811. DOI: http://doi.org/10.1017/S0890037X00028724 ZAND, E., N. NEZAMABADI, M.A. BAGHESTANI, P. SHIMI and S.K. MOUSAVI, 2019. A guide to chemical control of weeds in Iran. Jihad-e-Daneshgahi Press. Mashhad. pp. 154. (In Persian) ABBASPOOR, M., A.A. CHITBAND, M.R. MOLKARA and H. TAVAKOLI. 2013. Using isobolographic analysis for the evaluation of additive, synergism and antagonism effects in binary mixture of glyphosate and clopyralid on Acroptylon repense control. Iranian Plant Protection Research. 27: 294-300. DOI: http://doi.org/10.22067/jpp.v27i3.26731 ARMEL, G.R., P.L. RARDON, M.C. MCCOMRICK and N.M. FERRY, 2007. Differential response of several carotenoid biosynthesis inhibitors in mixtures with atrazine. Weed Technology. 21: 947-53. DOI: http://doi.org/10.1614/wt-06-133.1 ASHIGH, J. and J.C. HALL, 2010. Bases for interactions between saflufenacil and glyphosate in plants. Journal of Agricultural and Food Chemistry. 58: 7335-7343. DOI: http://doi.org/10.1021/jf100595a BARBIERI, G.F., B.G. YOUNG, F.E. DAYAN, J.C. STREIBIG, H.TAKANO, Jr.A. MEROTTO and L.A. AVILA, 2022. Herbicide mixtures: interactions and modeling. Advances in Weed Science. 40: e020220051. DOI: http://doi.org/ 10.51694/AdvWeedSci/2022;40:seventy-five011 BERENBAUM, M.C., 1981. Criteria for analyzing interactions between biologically active agents. Advances in Cancer Research. 35: 269-335. DOI: http://doi.org/10.1016/S0065-230X(08)60912-4 BESANÇON, T.E., D. PENNER and W.J. EVERMAN, 2018. Reduced translocation is associated with antagonism of glyphosate by glufosinate in giant foxtail (Setaria faberi) and velvetleaf (Abutilon theophrasti). Weed Science. 66: 159-167. DOI: 10.1017/wsc.2017.72 BLACKSHAW R.E. 1989. Synergistic mixes of DPX-A7881 and clopyralid in canola (Brassica napus). Weed Technology. 3: 690-695. DOI: http://doi.org/10.1017/s0890037x00033054 BUSI, R., T.A. GAINES and S. POWLES, 2017. Phorate can reverse P450 metabolism-based herbicide resistance in Lolium rigidum. Pest Management Science. 73: 410-417. DOI: http://doi.org/10.1002/ps.4441 CHITBAND, A.A., R. GHORBANI, M.H. RASHED MOHASSEL and M. NABIZADE. 2018. Joint action of some usable important broadleaf herbicides in sugar beet. Iranian Plant Protection Research. 31: 374-387. DOI: http://doi.org/10.22067/jpp.v31i3.50875 CHITBAND, A.A., R. GHORBANI, M. NABIZADE and E. ZAIDALI, 2017. Efficacy of mixing common herbicides in control of broadleaf weeds in sugar beet (Beta vulgaris L.). Journal of Sugar Beet. 33: 91-102. DOI: http://doi.org/10.22092/jsb.2017.101878.1099 CHITBAND, A.A., V. SARABI and A. ALIVERDI, 2025. Joint action of common herbicides on the control of Alhagi pseudalhagi (Bieb.) Desv.: A comparison study. Weed Research. 65: e12679. DOI: http://doi.org/10.1111/wre.12679 DEVEIKYTE, I., L. SARUNAITE and V. SEIBUTIS, 2015. Evaluation of pre- and postemergence herbicide combinations for broadleaved weeds in sugar beet. In: PRICE, A., J. KELTON and L. SARUNAITE. Herbicides, agronomic crops and weed biology. IntechOpen. DOI: http://doi.org/10.5772/61437 FAO. 2023. Food and Agriculture Organization www.fao.org/faostat/en/#data/QC FINCH, B., H. WRIGHT-SMITH, J. DAVIS, AND B. SCOTT, 2024. Jar Test Instructions: Assessing Pesticide-Fertilizer Compatibility (Jar Test). University of Arkansas. FSA2166R. www.uaex.uada.edu/publications/PDF/FSA-2166.pdf HEAP I, 2025. The international herbicide-resistant weed database. www.weedscience.org HICKS, T.V., G.R. WEHTJE and T.L. GREY, 1998. The interaction of pyridate and 2,4-DB in peanut (Arachis hypogaea), Florida beggarweed (Desmodium tortuosum), and sicklepod (Senna obtusifolia). Weed Science. 46: 284-288. DOI: http://doi.org/10.1017/s0043174500089426 HOLLAWAY, K.L., N.D. HALLAM and A.G. FLYNN, 1996. Synergistic joint action of MCPA ester and metsulfuron-methyl. Weed Research. 36: 369-374. DOI: http://doi.org/ 10.1111/j.1365-3180.1996.tb01666.x HOSSEYNI, S.M., H. NAJAFI, B. SANI and H. MOZAFARI, 2022. Investigation the effect of the application of single and mixing of propyzamide and ethofumesate herbicides in the control of dodder (Cuscuta campestris), grass and broad leaves weeds of sugar beet (Beta vulgaris L.). Journal of Crop Ecophysiology. 16: 563-580. DOI: http://doi.org/10.30495/JCEP.2023.1932247.1806 (in Persian with English summary) JING, X., G. YAO, D. LIU, M. LIU, P. WANG and Z. ZHOU, 2016. Environmental fate of chiral herbicide fenoxaprop-ethyl in water-sediment microcosms. Scientific Reports. 6: 26797. DOI: http://doi.org/10.1038/srep26797 KELLEY, K.B., L.M. WAX, A.G. HAGER and D.E. RIECHERS, 2005. Soybean response to plant growth regulator herbicides is affected by other postemergence herbicides. Weed Science. 53: 101-112. DOI: http://doi.org/10.1614/ws-04-078r LE, V.V., A.V. NGUYEN, D.T. LUU, F.B. FRITSCHI, C.T. NGUYEN, T.L. HO, 2024. Inhibitory effects of N-trans-cinnamoyltyramine on growth of invasive weeds and weedy rice. Plant-Environment Interactions. 5: e70017. DOI: 10.1002/pei3.70017 MAJIDI, M., G. HEIDARI and Y. EMAM, 2023. Qualitative characteristics of sugar beet as affected by different broadleaf herbicides combinations. Iran Agricultural Research. 36: 1-6. DOI: http://doi.org/10.22099/iar.2017.4126 (in Persian with English summary) MASABNI, J.G. and B.H. ZANDSTRA, 1999. Discovery of a common purslane (Portulaca oleracea) biotype resistant to linuron. Weed Technology. 13: 599-605. DOI: http://doi.org/10.1017/S0890037X00046261 OTTIS, B.V., J.D. MATTICE and R.E. TALBERT, 2005. Determination of antagonism between cyhalofop-butyl and other rice (Oryza sativa) herbicides in barnyardgrass (Echinochloa crus-galli). Journal of Agricultural and Food Chemistry. 53: 4064-068. DOI: http://doi.org/10.1021/ jf050006d OU, J., C.R. THOMPSON, P.W. STAHLMAN, N. BLOEDOW and M. JUGULAM, 2018. Reduced translocation of glyphosate and dicamba in combination contributes to poor control of Kochia scoparia: evidence of herbicide antagonism. Scientific Reports. 8: 1-11. DOI: http://doi.org/10.1038/ s41598-018-23742-3 PARADOWSKI, A. and T. PRACZYK, 2004. Evaluation of selected mixtures of active ingredients for weed control in sugar beet. Progress in Plant Protection. 44: 1004-1007. DOI: http://doi.org/10.24425/jppr.2023.146878 RASHIDI, S., A.R. YOUSEFI, N. GOICOECHEA, M. POURYOUSEF, P. MORADI, S. VITALINI and M. IRITI, 2021. Allelopathic interactions between seeds of Portulaca oleracea L. and crop species. Applied Sciences. 11: 3539. DOI: http://doi.org/10.3390/app11083539 RITZ, C., F. BATY, J.C. STREIBIG and D. GERHARD, 2015. Dose-response analysis using R. PLoS One. 10: e0146021. DOI: http://doi.org/10.1371/journal.pone.0146021 SCHUSTER, C.L., K. AL-KHATIB and J.A. DILLE, 2007. Mechanism of antagonism of mesotrione on sulfonylurea herbicides. Weed Science. 55: 429-434. DOI: 10.1614/ws-06-217.1 SØBYE, K.W., J.C. STREIBIG, N. CEDERGREEN, 2011. Prediction of joint herbicide action by biomass and chlorophyll a fluorescence. Weed Research. 5:23-32. DOI: http://doi.org/10.1111/j.1365-3180.2010.00824.x SOROKA, S.V. and G.J. GADZHIEVA, 2006. State of weed infestation and features of sugar beet protection in Belarus. Matica Srpska Journal for Natural Sciences. 110: 165-172. DOI: http://doi.org/10.2298/ZMSPN0610165S TEYMOURINIA, M., A.A. CHITBAND, G. REZAEE and S. KHAYRANDISH, 2023. The joint action of glyphosate, clethodim, and imazethapyr to control cogongrass (Imperata cylinderica L. Beauv) in the margin of the irrigation canals (A case study using two different approaches). Crop Protection. 174: 106413. DOI: http://doi.org/10.1016/j.cropro.2023.106413 WANAMARTA, G., D. PENNER and J.J. KELLS, 1989. The basis of bentazon antagonism on sethoxydim absorption and activity. Weed Science. 37: 400-404. DOI: http://doi.org/10.1017/s004317450007212x WILSON R.G., 1994. New herbicides for postemergence application in sugarbeet (Beta vulgaris). Weed Technology. 8: 807-811. DOI: http://doi.org/10.1017/S0890037X00028724 ZAND, E., N. NEZAMABADI, M.A. BAGHESTANI, P. SHIMI and S.K. MOUSAVI, 2019. A guide to chemical control of weeds in Iran. Jihad-e-Daneshgahi Press. Mashhad. pp. 154. (In Persian)
Statistics Article View: 233 PDF Download: 186

Journal management system. designed by sinaweb

Related Links

Evaluation the effect of binary mixing interactions of triflusulfuron-methyl, chloridazone, and clopyralid on common purslane (Portulaca oleracea L.) by isobole models