| تعداد نشریات | 284 |
| تعداد نشریات سازمان | 82 |
| تعداد شماره‌ها | 3,034 |
| تعداد مقالات | 33,949 |
| تعداد مشاهده مقاله | 45,378,618 |
| تعداد دریافت فایل اصل مقاله | 67,269,059 |
Genetic diversity of Lecanicillium fungicola causing button mushroom dry bubble disease using ISSR markers and evaluation of its sensitivity against essential oils and selected fungicides | ||
| Mycologia Iranica | ||
| دوره 12، شماره 1، شهریور 2025، صفحه 69-82 اصل مقاله (1.98 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22092/mi.2025.369172.1310 | ||
| نویسندگان | ||
| Farzaneh Memarzadeh1؛ Bahram Sharifnabi* 1؛ Rahim Eslamizadeh2 | ||
| 1Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan, IRAN | ||
| 2Jolgeh Dez Agricultural Company, Dezful, Khuzestan, Iran | ||
| چکیده | ||
| Dry bubble disease, caused by Lecanicillium fungicola, is a major challenge in button mushroom cultivation worldwide. This study examined the genetic diversity of L. fungicola isolates collected from various regions of Alborz, Isfahan, and Khuzestan provinces. Ten ISSR primers were employed to assess genetic diversity among the isolates. A total of 224 amplified fragments were obtained, with a similarity coefficient of 0.42 to 0.82 among isolates. Based on a similarity coefficient of 67%, the isolates were categorized into six groups. The average Polymorphism Information Content (PIC) was 0.772, with the highest PIC observed at 0.888, indicating a polymorphism rate of 96.8%. Furthermore, the efficacy of six commercial fungicides (including Trifloxystrobin, Copper Oxide, Iprodione + Carbendazim, Penconazole, Neem, and Carbendazim) as well as four plant essential oils (Thyme, Peppermint, Eucalyptus, and Rosemary) on mycelial growth of the isolates was assessed at various concentrations using the disc-diffusion method. The results revealed varying levels of sensitivity to fungicides, with the isolate exhibiting the highest growth rate also showing high sensitivity to the fungicides. Among the tested fungicides, Iprodione + Carbendazim exhibited the highest inhibition. Peppermint Essential oil at a concentration of 0.8 ppm had the highest mycelial growth inhibition. This study provides valuable insights into the genetic diversity of L. fungicola isolates, their various level of sensitivity to fungicides, and the potential use of essential oils in disease management. It offers a foundation for future research on resistance evolution and integrated disease control strategies. | ||
| کلیدواژه‌ها | ||
| Agaricus bisporus؛ Molecular marker؛ Chemical control؛ Plant extract | ||
| مراجع | ||
|
Anonymous. 2021a. Health Canada. https://pr-rp.hc-sc.gc.ca/mrl-lrm/
Anonymous. 2021b. European Commission. https://ec.europa.eu/food/plant/pesticides/eu-pesticides-database/active-substances/?event=search.as
Anonymous. 2022. American Mushroom Institute. https://www.americanmushroom.org/integrated-pest-management/fungicides
Allahyari M, Gholamnezhad J, Maleki M. 2021. Evaluation of the plant essential oils to control of dry bubble disease (Lecanicillium fungicola (Preuss) Zare) in the white button mushroom. Polish Journal of Natural Sciences. 36(1): 5-23. https://doi.org/10.31648/pjns.7303.
Baysal Ö, Mercati F, İkten H, Yıldız RÇ, Carimi F, Aysan Y, … et al. 2011. Clavibacter michiganensis subsp. michiganesis: Tracking strains using their genetic differentiations by ISSR markers in Southern Turkey. Physiological and Molecular Plant Pathology. 75(3): 113-119. https://doi.org/10.1016/J.PMPP.2010.10.002.
Berendsen RL, Baars JJ, Kalkhove SI, Lugones LG, Wösten HA, Bakker PA. 2010. Lecanicillium fungicola: causal agent of dry bubble disease in white‐button mushroom. Molecular Plant Pathology. 11(5): 585-595. 10.1111/j.1364-3703.2010.00627.x.
Bhat MA, Bhat Z, Khan N, Mohiddin F, Mir G. G. 2017. Response of fungicides against Verticillium fungicola and the host fungus Agaricus bisporus under in vitro conditions. Pesticide Research Journal. 29(1): 6-11.
Bonnen AM, Hopkins C. 1997. Fungicide resistance and population variation in Verticillium fungicola, a pathogen of the button mushroom, Agaricus bisporus. Mycological Research. 101(1): 89-96. https://doi.org/10.1017/S0953756296002237.
Caitano CE, Iossi MR, Pardo-Giménez A, Vieira Júnior WG, Dias ES, Zied DC. 2020. Design of a useful diagrammatic scale for the quantification of Lecanicillium fungicola disease in Agaricus bisporus cultivation. Current Microbiology. 77: 4037-4044. https://doi.org/10.1007/s00284-020-02232-8.
Chrysayi-Tokousbalides M, Kastanias M, Philippoussis A, Diamantopoulou P. 2007. Selective fungitoxicity of famoxadone, tebuconazole and trifloxystrobin between Verticillium fungicola and Agaricus bisporus. Crop Protection. 26(4): 469-475. https://doi.org/10.1016/j.cropro.2006.02.016.
Dianez F, Santos M, Parra C, Navarro MJ, Blanco R, Gea FJ. 2018. Screening of antifungal activity of 12 essential oils against eight pathogenic fungi of vegetables and mushroom. Letters in Applied Microbiology. 67(4): 400-410. https://doi.org/10.1111/lam.13053.
Fletcher J, Yarham D. 1976. The incidence of benomyl tolerance in Verticillium fungicola, Mycogone perniciosa and Hypomyces rosellus in mushroom crops. Annals of Applied Biology. 84(3): 343-353.
https://doi.org/10.1111/j.1744-7348.1976.tb01777.x.
Gea FJ, Navarro M, Santos M, Diánez F, Carrasco J. 2021. Control of fungal diseases in mushroom crops while dealing with fungicide resistance: A review. Microorganisms 9(3):585-609. https://doi.org/10.3390/microorganisms9030585.
Gea FJ, Carrasco J, Diánez F, Santos M, Navarro MJ 2014. Control of dry bubble disease (Lecanicillium fungicola) in button mushroom (Agaricus bisporus) by spent mushroom substrate tea. European Journal of Plant Pathology. 138: 711-720. https://doi.org/10.1007/s10658-013-0344-y.
Geösel A, Szabó A, Akan O, Szarvas J. 2014. Effect of essential oils on mycopathogens of Agaricus bisporus. Proceedings of 8th International Conference on Mushroom Biology and Mushroom Products (ICMBMP8). Delhi, India Vol. 2: 530-536.
Grogan H. 2008. Challenges facing mushroom disease control in the 21st century. 6th International Conference on Mushroom Biology and Mushroom Products. Bonn, Germany: p. 120-127.
Idrees M, Irshad M. 2014. Molecular markers in plants for analysis of genetic diversity: a review. European Academic Research. 2(1): 1513-1540.
Largeteau ML, Baars JP, Regnault-Roger C, Savoie J-M. 2006. Molecular and physiological diversity among Verticillium fungicola var. fungicola. Mycological Research. 110(4): 431-440. https://doi.org/10.1016/j.mycres.2005.11.016.
Largeteau ML, Mata G, Savoie J-M. 2008. Verticillium fungicola var. fungicola: comparison of some Mexican and French isolates. Revista Mexicana De Micología. 26:35-40.
Largeteau ML, Savoie J-M. 2008. Effect of the fungal pathogen Verticillium fungicola on fruiting initiation of its host, Agaricus bisporus. Mycological Research. 112(7): 825-828 https://doi.org/10.1016/j.mycres.2008.01.018.
Levi A, Thomas C. E, Simmons A. M, Thies J. A. 2005. Analysis based on RAPD and ISSR markers reveals closer similarities among Citrullus and Cucumis species than with Praecitrullus fistulosus (Stocks) Pangalo. Genetic Resources and Crop Evolution. 52(4): 465-472. https://doi.org/10.1007/s10722-005-2260-2.
Mehrparvar M, Goltapeh E. M, Safaie N, Ashkani S, Hedesh R. M. 2016. Antifungal activity of essential oils against mycelial growth of Lecanicillium fungicola var. fungicola and Agaricus bisporus. Industrial Crops and Products. 84: 391-398. https://doi.org/10.1016/j.indcrop.2016.02.012.
Mehrparvar M, Mohammadi Goltapeh E, Safaei N. 2013. Resistance of Iranian Lecanicillium fungicola to benzimidazole and ergosterol demethylation inhibiting fungicides. Journal of Agricultural Science and Technology. 15(2): 389-396.
Mehrparvar M, Mohammadi Goltapeh E, Safaie N. 2012. Evaluation of genetic diversity of Iranian Lecanicillium fungicola isolates using URP marker. Journal of Crop Protection. 1(3): 229-238.
Memarzadeh F, Sharifnabi B, Eslamizadeh R. 2020. Lecanicillium fungicola, the predominant cause of Agaricus bisporus basidiocarp deformity in Isfahan, Alborz and Khuzestan Provinces. Mycologia Iranica. 7(2): 259-261. https://doi.org/ 10.22092/mi.2020.124405.
Mitina G, Kazartsev I, Vasileva A, Yli‐Mattila T. 2017. Multilocus genotyping-based species identification of entomopathogenic fungi of the genus Lecanicillium (= Verticillium lecanii sl). Journal of Basic Microbiology. 57(11): 950-961. https://doi.org/10.1002/jobm.201700092.
Mohammadi Goltapeh E, Porjam E. 2001. Principles of edible mushrooms culture. Tarbiat Modares University Publication office of scientific works, Tehran, Iran.
Murray M, Thompson W. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research. 8(19): 4321-4326. https://doi.org/10.1093/nar/8.19.4321.
Navarro M J, Tello J C. 2005. Reduced sensitivity of the mushroom pathogen Verticillium fungicola to prochloraz-manganese in vitro. Mycological Research. 109(6): 741-745. https://doi.org/10.1017/S095375620500242X.
Rokni N, Sadati SAA, Safaie N, Ebrahimi MA, Samimifar M 2019. Assessment of genetic relatedness among commercial and wild strains of Agaricus bisporus using repetitive extragenic palindromic sequences and polymerase chain reaction. Mycoscience. 60(5): 293-297. https://doi.org/10.1016/j.myc.2019.06.002.
Rokni N, Zare R, Shafeinia A. 2020. Molecular detection of Lecanicillium fungicola on white button mushroom (Agaricus bisporus), using species-specific primer set. Rostaniha. 21(2): 206-217. https://doi.org/10.22092/botany.2020.343141.1203.
Santos TLd, Belan LL, Zied DC, Dias ES, Alves E.2017. Essential oils in the control of dry bubble disease in white button mushroom. Ciência Rural. 47. https://doi.org/10.1590/0103-8478cr20160780.
Singh M, Kamal S, Sharma VP. 2020. Status and trends in world mushroom production-III-world production of different mushroom species in 21st century. Mushroom Research. 29(2): 75. https://doi.org/10.36036/MR.29.2.2020.113703.
Singh S, Lal AA. 2019. In-vitro evaluation of selected fungicides against Verticillium fungicola in white button mushroom (Agaricus bisporus) cultivation. Think India Journal. 22(34): 1266-1272.
Singh S, Lal AA, Yaduman R, Simon S, Ramteke P. 2018. Genetic variability of strains of Verticillium fungicola causing dry bubble disease of white button mushroom Agaricus bisporus (Lange) Imbach. Annals of Plant Protection Sciences. 26(1): 137-141. https://doi.org/10.5958/0974-0163.2018.00030.7.
Siwulski M, Sobieralski K, Górski R, Lisiecka J, Sas-Golak I. 2011. Temperature and pH impact on the mycelium growth of Mycogone perniciosa and Verticillium fungicola isolates derived from polish and foreign mushroom growing houses. Journal of Plant Protection Research. 51(3): 268-272. https://doi.org/10.2478/v10045-0011-0044-6.
Sunaryo W, Idris SD, Pratama AN, Ratanasut K. 2020. Genetic relationships among cultivated and wild bananas from East Kalimantan, Indonesia based on ISSR markers. Biodiversitas Journal of Biological Diversity. 21(2): 824-832. https://doi.org/10.13057/biodiv/d210250.
Toledo AV, Franco ME, Medina R, de Remes Lenicov AMM, Balatti PA. 2019. Assessment of the genetic diversity of Argentinean isolates of Beauveria bassiana (Ascomycota: Hypocreales) using ISSR markers. Journal of King Saud University-Science. 31(4): 1264-1270. https://doi.org/10.1016/j.jksus.2018.02.006.
Zaker M. 2016. Natural plant products as eco-friendly fungicides for plant diseases control-a review. The Agriculturists. 14(1): 134 https://doi.org/10.3329/agric.v14i1.29111.
Zare R, Gams W. 2008. A revision of the Verticillium fungicola species complex and its affinity with the genus Lecanicillium. Mycological Research. 112(7): 811-824. https://doi.org/10.1016/j.mycres.2008.01.019.
Zied DC, Nunes JS, Nicolini VF, Gimenez AP, Rinker DL, Dias ES. 2015. Tolerance to Lecanicillium fungicola and yield of Agaricus bisporus strains used in Brazil. Scientia Horticulturae. 190: 117-122. https://doi.org/10.1016/j.scienta.2015.04.021 | ||
|
آمار تعداد مشاهده مقاله: 71 تعداد دریافت فایل اصل مقاله: 75 |
||