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In Silico genomic fingerprints of the pathogenic cyanobacterial strains isolated from Alma-Gol Wetland (Golestan Province, Iran), using highly repeated sequences | ||
| Rostaniha | ||
| دوره 26، شماره 1 - شماره پیاپی 72، دی 2025، صفحه 27-48 اصل مقاله (2.2 M) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/bot.j.iran.2025.369416.1420 | ||
| نویسندگان | ||
| Marzieh Samadi1؛ Hassan Beiranvand1؛ Morteza Mohajeri َAmiri2؛ Bahareh Nowruzi* 3 | ||
| 1MSc Student, Department of Biology, SR.C., Islamic Azad University, Tehran, Iran | ||
| 2Assistant Prof., Department of Biology, SR.C., Islamic Azad University, Tehran, Iran | ||
| 3Associate Prof., Department of Biology, SR.C., Islamic Azad University, Tehran, Iran | ||
| چکیده | ||
| Alma-Gol Wetland (Golestan Province, Iran) is a permanent habitat for the cultivation of many fish and aquatic organisms and is a popular destination for many travelers during the warm seasons of the year. The discovery of several dead fish carcasses on the water surface indicated the presence of cyanotoxins in the said area. Therefore, the present study, is aimed to screen potentially toxic cyanobacterial strains in this wetland by amplifying mcyG, mcyD, and mcyE toxic genes, as well as structural genes including 16S rRNA and ITS. It was also employed the rep-PCR technique to amplify highly repetitive genes HIP, STRR, and ERIC. The results obtained from the dendrogram based on the amplification of palindromic primers showed that, strains Nostoc sp. 9, Aliinostoc sp. 1, and Desmonostoc sp. 10 clustered together, while strains Calothrix sp. 7 and Neowestiellopsis sp. 2 mostly formed separate branches. However, only the strain Neowestiellopsis sp. 2 contained the toxic gene mcyD. Furthermore, comparative analysis of the ITS region length revealed that, both the length and the structure of the D1-D1 and BOX B helices differed from those of other strains. This study is among the first to demonstrate that fingerprinting techniques can provide new insights into different cyanobacterial strains. | ||
| کلیدواژه‌ها | ||
| Dendrogram؛ molecular phylogeny؛ palindromic primers؛ phylogenetic tree؛ toxic cyanobacteria | ||
| عنوان مقاله [English] | ||
| مطالعه انگشت‌نگاری ژنومی درون‌رایانه‌ای سویه‌های سیانوباکتریایی بیماری‌زا جداشده از تالاب آلماگل (استان گلستان) با استفاده از توالی‌های به شدت تکراری | ||
| نویسندگان [English] | ||
| مرضیه صمدی1؛ حسن بیرانوند1؛ مرتضی مهاجری‌امیری2؛ بهاره نوروزی3 | ||
| 1دانشجوی کارشناسی ارشد بخش زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 2استادیار بخش زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 3دانشیار بخش زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| چکیده [English] | ||
| تالاب آلماگل (استان گلستان)، محل دایمی پرورش بسیاری از ماهی‌ها و موجودات آبزی، محل بازدید عمومی بسیاری از گردشگران در فصول گرم سال است. شناسایی اجساد مرده چندین ماهی روی سطح آب نشان از حضور سیانوباکتری‌های بیماری‌زا (سیانوتوکسین‌ها) در این منطقه بود. به همین دلیل در تحقیق حاضر، با تکثیر ژن‌های بیماری‌زای mcyG، mcyD و mcyE و همچنین ژن‌های ساختاری از جمله 16S rRNA و ITS به همراه استفاده از روش rep-PCR برای تکثیر ژن‌های به شدت تکراری HIP، STRR و ERIC، به غربالگری سویه بالقوه سیانوباکتری‌های بیماری‌زای این تالاب پرداخته شد. نتایج به دست آمده از دندروگرام حاصل از تکثیر پرایمرهای پالیندرومیک نشان داد سویه‌های Nostoc sp. 9، Aliinostoc sp. 1 و Desmonostoc sp. 10 در کلاد مشترک و سویه‌های Calothrix sp. 7 و Neowestiellopsis sp. 2 در بیشتر موارد، انشعابات جداگانه‌ای را تشکیل دادند. با این حال، تنها سویه Neowestiellopsis sp. 2 حاوی ژن بیماری‌زای mcyD بود. همچنین، نتایج حاصل از آنالیز مقایسه طول مناطق ITS نشان داد که طول منطقه و همچنین ساختار مارپیچ‌های D1-D1 و BOX B با سایر سویه‌ها متفاوت بود. مطالعه حاضر جزو نخستین تحقیقات انجام شده است که نشان داد می‌توان با استفاده از روش انگشت‌نگاری، اطلاعات جدیدی را در زمینه سویه‌های مختلف سیانوباکتری ارایه نمود. | ||
| کلیدواژه‌ها [English] | ||
| پرایمرهای پالیندرومیک, درخت فیلوژنتیک, دندروگرام, سیانوباکتری‌های سمی, فیلوژنی مولکولی | ||
| مراجع | ||
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