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Endophytic fungi associated with chickpea (Cicer arietinum L.) in Iran | ||
| Mycologia Iranica | ||
| دوره 12، شماره 2، اسفند 2025، صفحه 105-122 اصل مقاله (1.84 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22092/mi.2026.371898.1340 | ||
| نویسندگان | ||
| Mohammad Hojjati1؛ Mohammad Javan-Nikkhah* 1؛ Mohammad Reza Bihamta2؛ Khalil-Berdi Fotouhifar1؛ Zahra Rastaghi1 | ||
| 1Department of Plant Protection, Faculty of Agriculture, University of Tehran, Karaj, Iran | ||
| 2Department of Agronomy, Faculty of Agriculture, University of Tehran, Karaj, Iran | ||
| چکیده | ||
| Chickpea (Cicer arietinum) is an important legume crop. This annual plant is widely cultivated across many regions of the world. A notable characteristic of chickpeas is their multiple symbiotic associations with diverse microorganisms that contribute to soil fertility. In this study, we investigated the diversity of endophytic fungi in seeds, aerial and underground organs of twenty-one genotypes of chickpea. The genotypes were obtained from the Dryland Agricultural Research Institute (Maragheh, East Azerbaijan, Iran). The genotypes were grown under both greenhouse and field conditions using disinfected and non-disinfected seeds. Additionally, to assess endophytic fungal diversity under field conditions, sampling was conducted in chickpea fields in the villages of Anjirak and Znylaan-Sofla, Kermanshah County, as well as in fields on the experimental campus of the Faculty of Agriculture, University of Razi, Kermanshah, Iran. A total of 366 fungal strains were isolated, of which 80 isolates were obtained from plants grown from non-disinfected and 60 isolates from disinfected seeds in the greenhouse. Moreover, from chickpea genotypes planted on a farm in Hamadan Province and 48 plant samples collected across six fields in Kermanshah Province, a total of 50 and 155 endophytic fungal isolates were obtained, respectively. Furthermore, a total of 21 fungal isolates were recovered from seeds of genotypes ‘Desi 37’, ‘Kaka’, ‘Sufi’, and ‘Flip 09-2780’. Following morphological characterization and sequencing of representative isolates based on the genomic regions tef1-α, tub2, ITS rDNA, cal and rpb2, ten fungal species were identified, belonging to six genera. The identified species were Allophoma labilis, Aspergillus fumigatus, A. luchuensis, A. niger, A. tubingensis, Chaetomium rectangulare, Cladosporium ramotenellum, Fusarium acuminatum, F. redolens and Penicillium chrysogenum. The most isolates belong to Aspergillus (60.65%) and Fusarium species (27.86%). Among the chickpea genotypes, the greatest number of isolates were obtained from ‘Flip 09-2780’ (43 isolates) and ‘Adel’ (41 isolates). | ||
| کلیدواژهها | ||
| greenhouse؛ field؛ morphology؛ phylogeny؛ diversity | ||
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