اخبار و اعلانات

 آمار

تعداد نشریات285
تعداد نشریات سازمان83
تعداد شماره‌ها3,139
تعداد مقالات34,918
تعداد مشاهده مقاله50,383,514
تعداد دریافت فایل اصل مقاله90,276,872
A Waheeb, Saad, Noori, Ali Mohammed, Hussein, Hussein Khaleel, Omar, Jalal Karim, Abdelsalam, Nader R.. (1404). Evaluation of Natural Essential Oils as Sustainable Disinfectants for the Control of Microbial Contaminants in Vitro Plant Tissue Culture. سامانه مدیریت نشریات علمی, 15(1), 146-152. doi: 10.22034/jmpb.2026.371791.2121
Saad A Waheeb; Ali Mohammed Noori; Hussein Khaleel Hussein; Jalal Karim Omar; Nader R. Abdelsalam. "Evaluation of Natural Essential Oils as Sustainable Disinfectants for the Control of Microbial Contaminants in Vitro Plant Tissue Culture". سامانه مدیریت نشریات علمی, 15, 1, 1404, 146-152. doi: 10.22034/jmpb.2026.371791.2121
A Waheeb, Saad, Noori, Ali Mohammed, Hussein, Hussein Khaleel, Omar, Jalal Karim, Abdelsalam, Nader R.. (1404). 'Evaluation of Natural Essential Oils as Sustainable Disinfectants for the Control of Microbial Contaminants in Vitro Plant Tissue Culture', سامانه مدیریت نشریات علمی, 15(1), pp. 146-152. doi: 10.22034/jmpb.2026.371791.2121
A Waheeb, Saad, Noori, Ali Mohammed, Hussein, Hussein Khaleel, Omar, Jalal Karim, Abdelsalam, Nader R.. Evaluation of Natural Essential Oils as Sustainable Disinfectants for the Control of Microbial Contaminants in Vitro Plant Tissue Culture. سامانه مدیریت نشریات علمی, 1404; 15(1): 146-152. doi: 10.22034/jmpb.2026.371791.2121

Evaluation of Natural Essential Oils as Sustainable Disinfectants for the Control of Microbial Contaminants in Vitro Plant Tissue Culture

Journal of Medicinal plants and By-products
مقاله 20، دوره 15، شماره 1، فروردین و اردیبهشت 2026، صفحه 146-152    اصل مقاله (9.4 M)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22034/jmpb.2026.371791.2121
نویسندگان
Saad A Waheeb* 1؛ Ali Mohammed Noori1؛ Hussein Khaleel Hussein1؛ Jalal Karim Omar2؛ Nader R. Abdelsalam3
1Department of Horticulture and Landscape Design, College of Agriculture, Kirkuk University, Kirkuk, Iraq
2Ministry of Health, Sulaymaniyah General Directorate of Health, Blood Bank Directorate, Sulaymaniyah, Iraq
3Agricultural Botany Department, Faculty of Agriculture, (Saba Basha), Alexandria University, Alexandria, Egypt
چکیده
The current investigation assessed the impact of five natural essential oils on the in vitro sterilization effectiveness of different explants. The different explants were subjected to three concentrations (1%, 2%, 3% v/v) and three exposure times (1, 2, and 3 min). In addition, the sterilization rates were assessed after 14 days. Results explained that sterilization effectiveness was clearly affected by both oil type and treatment factors. At 1% concentration, eucalyptus and neem attained the highest early sterilization in Bougainvillea (83.3% and 76.0%, respectively), though peppermint and clove confirmed lower activity (56.0% and 66.7%, respectively). The results indicated that increasing the exposure time to 3 min usually increased sterilization rates, with clove, eucalyptus, peppermint, thyme, and neem oils succeeding in 89 to 100% sterilization through species but caused loss of viability. At 2% concentration and 2 min exposure, most oils efficiently sterilized explants while maintaining tissue viability; for example, A. julibrissin explants reached 83.3 to 100% sterilization without death, whereas 3% concentration often caused explant death, mainly in Bougainvillea and R. chinensis minima. Thyme and neem oils reliably confirmed the highest and most reliable activity, attaining 100% sterilization through concentrations and exposure times in R. chinensis minima and A. julibrissin, emphasizing their broad-spectrum antimicrobial potential. Clove required greater concentrations to reach similar efficacy due to its eugenol content, whereas eucalyptus and peppermint performed moderately well at lower concentrations. Based on these results, a recommended sterilization protocol for in vitro culture means using 2% essential oil concentration with 2-minute exposure time.
کلیدواژه‌ها
Tissue culture؛ Essential oils؛ In vitro؛ Sterilization
مراجع
  1. Newman D.J., Cragg G.M. Natural products as sources of new drugs from 1981 to 2014. Journal of Natural Products. 2016; 79(3): 629-661.
  2. Newman D.J., Cragg G.M. Natural products as sources of new drugs over the nearly four decades from 01/1981 to 09/2019. Journal of Natural Products. 2020;83(3): 770-803.
  3. Hyldgaard M., Mygind T., Meyer R.L. Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components. Frontiers in Microbiology. 2012;3: 12.
  4. Mutlu-İngok A., Devecioglu D., Dikmetas D.N., Karbancioglu-Guler F., Capanoglu E. Antibacterial, antifungal, antimycotoxigenic, and antioxidant activities of essential oils: An updated review. Molecules. 2020; 25(20): 4711. https://doi.org/10.3390/molecules25204711
  5. Nakhaei F. Identification of essential oil components, total phenolic content and antioxidant properties of Mentha aquatica in Southern Khorasan Province, Iran. Journal of Medicinal Plants and By-products. 2024; 13(1): 43–49. doi:10.22092/jmpb.2022.354236.1354
  6. Rao J., Chen B., McClements D.J. (2019). Improving the efficacy of essential oils as antimicrobials in foods: Mechanisms of action. Annual Review of Food Science and Technology. 2019; 10(1): 365-387.
  7. Sánchez E., García S., Heredia N. Extracts of edible and medicinal plants damage membranes of Vibrio cholerae. Applied and Environmental Microbiology. 2010; 76(20): 6888-6894.
  8. Bolouri, P., Salami, R., Kouhi, S., Kordi, M., Asgari Lajayer, B., Hadian, J., & Astatkie, T. Applications of essential oils and plant extracts in different industries. Molecules. 2022; 27(24): 8999. https://doi.org/10.3390/molecules27248999
  9. Chaghakaboodi Z., Kahrizi D., Nasiri J. Chemical composition and antimicrobial potential of Eucalyptus camaldulensis essential oil from Shushtar, Iran. Journal of Medicinal Plants and By-products. 2024; 13(3): 691–702. doi:10.22034/jmpb.2024.365950.1700
  10. Tajkarimi M.M., Ibrahim S.A., Cliver D.O. Antimicrobial herb and spice compounds in food. Food Control. 2010; 21(9): 1199-1218.
  11. Burt S. Essential oils: their antibacterial properties and potential applications in foods-a review. International Journal of Food Microbiology. 2004;94(3): 223-253.
  12. George D.R., Smith T.J., Shiel R.S., Sparagano O.A.E., Guy J.H. Mode of action and variability in efficacy of plant essential oils showing toxicity against the poultry red mite, Dermanyssus gallinae. Veterinary Parasitology. 2009;161(3-4): 276-282.
  13. Kim S.I., Roh J.Y., Kim, D.H., Lee H.S., Ahn Y.J. (2003). Insecticidal activities of aromatic plant extracts and essential oils against Sitophilus oryzae and Callosobruchus chinensis. Journal of Stored Products Research. 2003; 39(3): 293-303.
  14. Astani A., Reichling J., Schnitzler P. Screening for antiviral activities of isolated compounds from essential oils. Evidence‐Based complementary and Alternative Medicine. 2011;2011(1): 253643.
  15. Silva F., Ferreira S., Duarte A., Mendonca D.I., Domingues F.C. Antifungal activity of Coriandrum sativum essential oil, its mode of action against Candida species and potential synergism with amphotericin B. Phytomedicine. 2011;19(1): 42-47.
  16. Brenes A., Roura E. Essential oils in poultry nutrition: Main effects and modes of action. Animal Feed Science and Technology. 2009; 158(1-2): 1-14.
  17. Ahmadifar E., Falahatkar, B., Akrami R. Effects of dietary thymol‐carvacrol on growth performance, hematological parameters and tissue composition of juvenile rainbow trout, Oncorhynchus mykiss. Journal of Applied Ichthyology. 2011; 27(4): 1057-1060.
  18. Kamal T.F., Al-saad K.G., Noori A.M. Initiation and Multiplication of Petunia Explants (Petunia hybrida L.) in Vitro Propagation Initiation and Multiplication of Petunia Explants (Petunia hybrida L.) in Vitro Propagation. IOP Conf. Series: Earth and Environmental Science. 2024;13(1371): 042054. https://doi.org/10.1088/1755-1315/1371/4/042054
  19. Abdulraheem Z.H., Noori A.M., Husni H.S., Lateef M.A., Alsaffar M.F. Digitalis purpurea L. Propagated in Vitro with Various Hormones Digitalis purpurea L. Propagated in Vitro with Various Hormones. IOP Conf. Series: Earth and Environmental Science. 2024; 13(1371): 042452. https://doi.org/10.1088/1755-1315/1371/4/042050
  20. Mosa N. Assessment Genetic Stability of Magnolia Grandiflora L. By Aflp Marker Via Tissue Culture Technology. Kirkuk University Journal for Agricultural Sciences. 2024; 15(4): 38-45. doi: 10.58928/ku24.15405
  21. Hesami M., Naderi R., Tohidfar M. Modeling and Optimizing in vitro Sterilization of Chrysanthemum Leaf Explants Using MLP-NSGAII. Frontiers in Plant Science. 2019; 10, 282. https://doi.org/10.3389/fpls.2019.00282
  22. Jasim N.S., Salih A.M., Ati M.A. Evaluating the efficiency of plant essential oils against common fungal contamination affecting tissue culture of date palms (Phoenix dactylifera L.) by in vitro culture. Research Journal of Chemistry and Environment. 2021; 25(6).
  23. Eliwa G.I., et al. In vitro explant surface sterilization and bud proliferation responses in plant micropropagation. Scientific Reports. 2024; 14:456. https://doi.org/10.1038/s41598-024-55685-3
  24. Bakkali F., Averbeck S., Averbeck D., Idaomar M. Biological effects of essential oils–a review. Food and Chemical Toxicology. 2008; 46(2): 446-475.
  25. Lambert R.J.W., Skandamis P.N., Coote P.J., Nychas G.J. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. Journal of Applied Microbiology. 2001; 91(3):453-462.
  26. Kasavi C., Finore I., Lama L., Nicolaus B., Oliver S.G., Oner, E. T., & Kirdar, B. (2012). Evaluation of industrial Saccharomyces cerevisiae strains for ethanol production from biomass. Biomass and Bioenergy. 2012; 45:230-238.
  27. Marchese A., Arciola C.R., Coppo E., Barbieri R., Barreca D., Chebaibi S., Daglia M., et al. The natural plant compound carvacrol as an antimicrobial and anti-biofilm agent: Mechanisms, synergies and bio-inspired anti-infective materials. Biofouling. 2018;34(6): 630-656.
  28. Mirzania F., Ghasemian Yadegari J., Salimikia I., Sarrafi Y., Aliahmadi A., Aalaei J. Variation of Eryngium caeruleum essential oil content and biological activity in wild and cultivated conditions. Journal of Medicinal Plants and By-products. 2024; 13(2): 439–446. doi:10.22034/jmpb.2023.361961.1544
  29. Biswas K., Chattopadhyay I., Banerjee R.K., Bandyopadhyay U. Biological activities and medicinal properties of neem (Azadirachta indica). Current Science. 2002; 1336-1345.
  30. Hasan H.A., Raauf A.M.R., Razik B.M. A., Hassan B.A.R. Chemical composition and antimicrobial activity of the crude extracts isolated from Zingiber officinale by different solvents. Pharmaceut Anal Acta. 2012; 3(9): 1-5.
  31. Settanni L., Palazzolo E., Guarrasi V., Aleo A., Mammina C., Moschetti G., Germanà M.A. Inhibition of foodborne pathogen bacteria by essential oils extracted from citrus fruits cultivated in Sicily. Food Control. 2012; 26(2): 326-330.
  32. Mohamad Zen N.I., Zaidan U.H., Abd. Gani S.S., Shamsi S., Hassan M., Marikkar N. Exploring the therapeutic potential of Stevia rebaudiana Bertoni leaves essential oil: biological effects for topical applications. Journal of Medicinal Plants and By-products. 2024; 13(3): 504–512. doi:10.22034/jmpb.2024.361730.1541
آمار
تعداد مشاهده مقاله: 98
تعداد دریافت فایل اصل مقاله: 58


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب