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GENETIC VARIATION AMONG NATURAL POPULATIONS OF AGRPYRON CRISTAUM (POACEAE) BASED ON SDS-PAGE OF SEED PROTEINS | ||
| The Iranian Journal of Botany | ||
| مقاله 7، دوره 19، شماره 2، اسفند 2013، صفحه 186-193 اصل مقاله (759.83 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/ijb.2013.4156 | ||
| نویسندگان | ||
| Mehdi Yousofi* 1؛ Mehrnoosh Esmaeili1؛ Mahmood Otroshy2 | ||
| 1Department of Biology, Payam Noor University, Tehran, Iran. | ||
| 2Agricultural Biotechnology Research Institute -Central region of Iran (ABRII) | ||
| چکیده | ||
| SDS-PAGE of seed proteins in 11 natural populations of Agropyron cristatum and one cultivated population of Elymus junceus was studied. In total 31 protein bands of different molecular weights were detected on the polyacrylamide gel indicating a high diversity. By performing Cluster Analysis and Principle Coordinate Analysis on these data, all studied populations were grouped in three major clusters. Clear correlation was seen between the population in three clusters with their geographical localities. This result suggests that seed protein markers between the natural populations of Agropyron cristatum are mainly correlated with eco-geographical factors and cannot be used alone for its taxonomy in the infra-specific levels. | ||
| کلیدواژه‌ها | ||
| Agropyron cristatum؛ cluster analysis؛ Iran؛ SDS PAGE؛ Seed protein | ||
| عنوان مقاله [English] | ||
| تنوع ژنتیکی بین جمعیت های خودروی Agropyron cristatum براساس الکتروفورز پروتئین‌های دانه | ||
| نویسندگان [English] | ||
| مهدی یوسفی1؛ مهرنوش اسماعیلی1؛ محمود اوتروشی2 | ||
| 1دانشیار گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه پیام نور، تهران. | ||
| 2مؤسسه تحقیقات فناوری کشاورزی، منطقه مرکزی ایران، نجف آباد. | ||
| چکیده [English] | ||
| پروتئین‌های ذخیره‌ای دانه 11 جمعیت خودروی Agropyron cristatum و یک جمعیت کاشته شدهElymus junceus از طریق الکتروفورز روی ژل پلی آکریل آمید بررسی شدند. در مجموع 31 باند پروتئینی با وزنهای مولکولی متفاوت روی ژل ظاهر شد که نشانگر تنوع بالا است. با انجام آنالیز خوشه‌ای و آنالیز مولفه‌های اصلی روی داده‌های به دست آمده، تمام جمعیت‌های مورد مطالعه در سه خوشه گروه بندی شدند. ارتباط آشکاری بین تاکسونهای هر خوشه و مکانهای جغرافیائی آنها مشاهده شد. نتایج پیشنهاد می‌کند که مارکرهای پروتئینی دانه جمعیت های خودروی Agropyron cristatum عمدتا با عوامل اکوجغرافیائی همبستگی دارند و به تنهائی نمی‌توانند برای تاکسونومی این گونه در سطوح زیرگونه‌ای مفید واقع شوند. | ||
| کلیدواژه‌ها [English] | ||
| Agropyron cristatum, cluster analysis, Iran, SDS PAGE, Seed protein | ||
| مراجع | ||
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