Omidi, M., Behjat Sasan, B., Naghavi, M., Kalate Jari, S., Etminan, A. (2011). Effect of growth regulators, medium and explant on callus induction in Taxus baccata L.. , 27(2), 316-325. doi: 10.22092/ijmapr.2011.6447
M. Omidi; B. Behjat Sasan; M.R. Naghavi; S. Kalate Jari; A.R. Etminan. "Effect of growth regulators, medium and explant on callus induction in Taxus baccata L.". , 27, 2, 2011, 316-325. doi: 10.22092/ijmapr.2011.6447
Omidi, M., Behjat Sasan, B., Naghavi, M., Kalate Jari, S., Etminan, A. (2011). 'Effect of growth regulators, medium and explant on callus induction in Taxus baccata L.', , 27(2), pp. 316-325. doi: 10.22092/ijmapr.2011.6447
Omidi, M., Behjat Sasan, B., Naghavi, M., Kalate Jari, S., Etminan, A. Effect of growth regulators, medium and explant on callus induction in Taxus baccata L.. , 2011; 27(2): 316-325. doi: 10.22092/ijmapr.2011.6447

Effect of growth regulators, medium and explant on callus induction in Taxus baccata L.

تحقیقات گیاهان دارویی و معطر ایران
Article 13, Volume 27, Issue 2 - Serial Number 52, July 2011, Pages 316-325    PDF (288.31 K)
Document Type: Research Paper
DOI: 10.22092/ijmapr.2011.6447
Authors
M. Omidi1; B. Behjat Sasan* 2; M.R. Naghavi1; S. Kalate Jari2; A.R. Etminan3
1Department of Agronomy and Plant Breeding, University of Tehran, Tehran, Iran
2Department of Horticultural, Islamic Azad University, Tehran, Iran
3Department of Agronomy and Plant Breeding,Islamic Azad University, Kermanshah, Iran
Abstract
Taxus baccata L. is an endangered forest tree species. Taxol is recognized as a highly effective anticancer drug. It is mainly extracted from the bark and needles of taxus. A factorial experiment was carried out on the base of completely randomized design to find the best combination of media, explants and plant regulators for callus induction in order to produce biomass taxol. In this research three factors were used as follows: MS medium and 4-modified MS media, which differ in salts and nitrogen construction and glutamine, two plant regulators (NAA, 2, 4-D and Kin), and two explants (stem and leaf).  Following two months of callus, the tissue was transferred to ½ MS supplemented with 0.4 mg/l 2, 4-D+ 3mg/l Kin. The maximum percentage of callus induction (96.97%) was obtained from stem segments on ½ MS (475 mg/l KNO3, 412.5 mg/l NH4NO3) medium in combination with glutamine (100 mg/l) and containing 2,4-D (1mg/l) + Kin (1mg/l). The best response (80.67 mm2) was observed from leaf on ½ MS in combination with glutamine (100 mg/l) and containing NAA (2mg/l)+ Kin (0.2 mg/l) for callus size. Stem callus showed meristematic cells. According to the results, glutamine showed significant effects on callus induction and growth. The effect of low concentrations of growth regulators on callus induction and callus size was also more than that of high concentrations of growth regulators.
Keywords
Taxus baccata L; growth regulators; Callus; MS
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