Roohbakhsh, A., Karimi, G. (2009). In vitro evaluation of xanthine oxidase inhibitory activity of aqueous extract of seven medicinal herbs. , 25(2), 139-148. doi: 10.22092/ijmapr.2009.7185
A. Roohbakhsh; G. Karimi. "In vitro evaluation of xanthine oxidase inhibitory activity of aqueous extract of seven medicinal herbs". , 25, 2, 2009, 139-148. doi: 10.22092/ijmapr.2009.7185
Roohbakhsh, A., Karimi, G. (2009). 'In vitro evaluation of xanthine oxidase inhibitory activity of aqueous extract of seven medicinal herbs', , 25(2), pp. 139-148. doi: 10.22092/ijmapr.2009.7185
Roohbakhsh, A., Karimi, G. In vitro evaluation of xanthine oxidase inhibitory activity of aqueous extract of seven medicinal herbs. , 2009; 25(2): 139-148. doi: 10.22092/ijmapr.2009.7185

In vitro evaluation of xanthine oxidase inhibitory activity of aqueous extract of seven medicinal herbs

تحقیقات گیاهان دارویی و معطر ایران
Article 2, Volume 25, Issue 2 - Serial Number 44, August 2009, Pages 139-148    PDF (247.82 K)
Document Type: Research Paper
DOI: 10.22092/ijmapr.2009.7185
Authors
A. Roohbakhsh* 1; G. Karimi2
1Department of Physiology and Pharmacology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
2Department of Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract
Over production of uric acid by xanthine oxidase (XO) causes gout. XO inhibitors for example allopurinol are the most important available anti gout drugs. Medicinal herbs are available natural sources that may be useful for the treatment of gout. In this study the inhibitory activity of aqueous extracts of Matricaria chamomilla L., Hypericum perforatum L., Fraxinus excelsior L., Zea mays L., Trachyspermum copticum (L.) Link, Cynara scolymus L. and Hedera helix L. were measured. In these experiments, under controlled conditions xanthine turns into uric acid by XO. Uric acid absorbance was measured at 295 nm using a UV spectrophotometer. Adding allopurinol (as positive control) or aqueous extracts to the solution containing XO, can decrease uric acid production by inhibition of this enzyme. At first, XO inhibitory activity of allopurinol and reproducibility of the method was evaluated by conducting three experiments. The results showed an EC50= 0.43 μg/ml for allopurinol. Then, XO inhibitory activity of aqueous extracts at 0.1, 0.5, 1, 1.5, 2 and 3 mg/ml were measured. Matricaria chamomilla could inhibit enzyme up to 68% (P< 0.001) while maximum XO inhibitory activities of Hypericum perforatum and Cynara scolymus were 36% (P< 0.001) and 21% (P< 0.001) respectively. Other extracts did not have any significant effect on XO. Our obtained results showed that part of anti gout effects of Matricaria chamomilla, Hypericum perforatum and Cynara scolymus is due to XO inhibition.
Keywords
Matricaria chamomilla L; Hypericum perforatum L; Fraxinus excelsior L; Zea mays L; Trachyspermum copticum (L.) Link; Cynara scolymus L; Hedera helix L
References
- Akdemir, H., Aşik, Z., Paşaoğlu, H., Karaküçük, I., Oktem, I.S. and Koç, R.K., 2001. The effect of allopurinol on focal cerebral ischaemia: an experimental study in rabbits. Neurosurgery Review, 24(2-3): 131-135.
- Bosisio, E., Mascetti, D. and Caballion, P., 2000. Screening of plants from New caledonia and Vanuatu for inhibitory activity of Xanthine oxidase and elastase. Pharmaceutical Biology, 38(1): 18-24.
- Costantino, L., Rastelli, G. And Albasini, A., 1992a. Inhibitory activity of flavonols towards the xanthine oxidas enzyme. International Journal of Pharmaceutics, 86: 17-23.
- Costantino, L., Albasini, A., Rastelli, G. and Benvenuti, S., 1992b. Activity of polyphenolic crude extracts as scavengers of superoxide radicals and inhibitors of xanthine oxidase. Planta Medica, 58(4): 342-344.
- Costantino, L., Rastelli, G. and Albasini, A., 1995. Anthocyanidines as inhibitors of xanthine oxidase. Pharmazie, 50(8): 573-574.
- Cos, P., Ying, L., Calomme, M., Hu, J.P., Cimanga, K., Van Poel, B., Pieters, L., Vlietinck, A.J. and Vanden Berghe, D., 1998. Structure-activity relationship and classification of flavonoids as inhibitors of xanthine oxidase and superoxide scavengers. Journal of Natural Products, 61(1): 71-76.
- Duke, J., 2001. Hand book of Medicinal Herbs. CRC Press, Washington D.C., 893p.
- Fauci, A., Kasper, D., Longo, D., Braunwald, E., Hauser, S., Jameson, J. and Loscalzo, J., 2008. Harrison’s Principle of Internal Medicine. McGraw Hill, New York, 2674p.
- González, A.G., Bazzocchi, I.L., Moujir, L., Ravelo, A.G., Correa, M.D. and Gupta, M.P., 1995. Xanthine oxidase inhibitory activity of some Panamanian plants from Celastraceae and Lamiaceae. Journal of Ethnopharmacology, 46(1): 25-29.
- Gruenwald, J., Brendler, T. and Jaenicke, C., 2000. PDR for Herbal Medicines. Medical Economics, Montvale, 1108p.
- Hatano, T., Yasuhara, T., Fukuda, T., Noro, T. and Okuda, T., 1989. Phenolic constituents of licorice. II. Structures of licopyranocoumarin, licoarylcoumarin and glisoflavone, and inhibitory effects of licorice phenolics on xanthine oxidase. Chemical and Pharmaceutical Bulletin, 37(11): 3005-3009.
- Hayashi, T., Nagayama, K., Arisawa, M., Shimizu, M., Suzuki, S., Yoshizaki, M., Morita, N., Ferro, E., Basualdo, I. and Berganza, L.H., 1989. Pentagalloylglucose, a xanthine oxidase inhibitor from a Paraguayan crude drug, "Molle-i" (Schinus terebinthifolius). Journal of Natural Products, 52(1): 210-211.
- Katzung, B., 2007. Basic & Clinical Pharmacology. McGraw Hill, New York, 1082p.
- Kong, L.D., Cai, Y., Huang, W.W., Cheng, CH. and Tan, R.X., 2000. Inhibition of xanthine oxidase by some Chinese medicinal plants used to treat gout. Journal of Ethnopharmacology, 73(1-2): 199-207.
- Lin, C.C., Yen, F.L., Hsu, F.F. and Lin, J.M., 2000. Anti-hypercholesterolaemia, antioxidant activity and free radical scavenger effects of traditional Chinese medicine prescriptions used for stroke. The Journal of Pharmacy and Pharmacology, 52(11): 1387-1393.
- Lio, M., Moriyama, A., Matsumoto, Y., Takakin, N. and Fukumoto, M., 1985. Inhibiton of xanthine oxidase by flavonoids. Agricultural and Biological Chemistry, 49(7): 2173-2176.
- Nakanishi, T., Nishi, M., Inada, A., Obata, H., Tanabe, N., Abe, S. and Wakashiro, M., 1990. Two new potent inhibitors of xanthine oxidase from leaves of Perilla frutescens (L.) Britton var. acuta Kudo. Chemical and Pharmaceutical Bulletin, 38(6): 1772-1774.
- Owen, P.L. and Johns, T., 1999. Xanthine oxidase inhibitory activity of northeastern North American plant remedies used for gout. Journal of Ethnopharmacology, 64(2): 149-160.
- Presibella, M., Santos, C. and Weffort-Santos, A., 2007. In Vitro Antichemotactic Activity of Chamomilla recutita Hydroethanol Extract. Pharmaceutical Biology, 45(2): 124 - 130
- Sheu, S.Y. and Chiang, H.C., 1996. Inhibitory effects of plant growth regulators on xanthine oxidase. Anticancer Research, 16(1): 311-315.
- Sarawek, S., Feistel, B., Pischel, I. and Butterweck, V., 2008. Flavonoids of Cynara scolymus possess potent xanthin oxidase inhibitory activity in vitro but are devoid of hypouricemic effects in rats after oral application. Planta Medica, 74(3): 221-227.
- Sweeney, A.P., Wyllie, S.G., Shalliker, R.A. and Markham, J.L., 2001. Xanthine oxidase inhibitory activity of selected Australian native plants. Journal of Ethnopharmacology, 75(2-3): 273-277.
- Zhou, C.X., Tanaka. J., Cheng, C.H., Higa, T. and Tan, R.X., 1999. Steroidal Alkaloids and Stilbenoids from Veratrum taliense. Planta Medica, 65(5): 480-482.
- Wede, I., Altindag, Z.Z., Widner, B., Wachter, H. and Fuchs, D., 1998. Inhibition of xanthine oxidase by pterins. Free Radical Research, 29(4): 331-338.
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