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اثر متیلجاسمونات بر آنزیمهای متابولیسمی و مواد فنلی در گیاه دارویی آگاستاکه (Agastache foeniculum [Pursh] Kuntze) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 3، دوره 30، شماره 3 - شماره پیاپی 65، مرداد 1393، صفحه 361-369 اصل مقاله (302.71 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2014.7500 | ||
| نویسندگان | ||
| فاطمه رؤففرد1؛ مظفر شریفی* 2؛ رضا امیدبیگی3؛ فاطمه سفیدکن4؛ مهرداد بهمنش5؛ نوراله احمدی6 | ||
| 1دانشجوی دکترا، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تربیت مدرس | ||
| 2دانشیار، گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس | ||
| 3استاد، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تربیت مدرس | ||
| 4استاد، مؤسسه تحقیقات جنگلها و مراتع کشور | ||
| 5دانشیار، گروه ژنتیک، دانشکده علوم زیستی، دانشگاه تربیت مدرس | ||
| 6استادیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تربیت مدرس | ||
| چکیده | ||
| تأثیر متیلجاسمونات بر فعالیت آنزیمهای فنیلآلانینآمونیولیاز (PAL) و 4-کوماراتکوآلیگاز (4CL)، مقدار فنل کل و مقدار پروتئین کل در گیاه دارویی آگاستاکه (Agastache foeniculum [Pursh] Kuntze) بررسی شد. آزمایشها در قالب طرح کاملاً تصادفی و در شرایط هیدروپونیک انجام شد و گیاهان در معرض غلظتهای مختلف متیلجاسمونات (0، 1/0 و 1 میلیمولار) قرار گرفتند. بعد از گذشت 24 ساعت از شروع تیمار، فعالیت آنزیم PAL در گیاهان تیمار شده با 1 میلیمولار متیل جاسمونات، در مقایسه با گیاهان تیمار شده با 1/0 میلیمولار متیلجاسمونات و گیاهان شاهد، بهطور معنیداری افزایش یافت. فعالیت آنزیم 4CL نیز بعد از گذشت 24 ساعت از تیمار با 1 میلیمولار متیلجاسمونات، در مقایسه با تیمار 1/0 میلیمولار متیلجاسمونات و تیمار شاهد، بهطور معنیداری افزایش یافت. با وجود این تیمارهای متیلجاسمونات هیچ اثر معنیداری بر مقدار فنل کل بعد از گذشت 8، 12 و 24 ساعت از تیمار در مقایسه با تیمارهای شاهد هر یک از زمانهای یاد شده نداشتند. مقدار پروتئین کل با تیمارهای 1 یا 1/0 میلیمولار متیلجاسمونات بعد از گذشت 24 ساعت از تیمار در مقایسه با شاهد، بهطور معنیداری افزایش یافت. | ||
| کلیدواژهها | ||
| متیلجاسمونات؛ آگاستاکه (Agastache foeniculum [Pursh] Kuntze)؛ فنیلآلانینآمونیالیاز؛ 4-کوماراتکوآلیگاز؛ مواد فنلی | ||
| عنوان مقاله [English] | ||
| Effect of methyl jasmonate on metabolic enzymes and phenolics, in Agastache foeniculum [Pursh] Kuntze | ||
| نویسندگان [English] | ||
| F. Raouf Fard1؛ M. Sharifi2؛ R. Omidbaigi3؛ F. Sefidkon4؛ M. Behmanesh5؛ N. Ahmadi6 | ||
| 1PhD. Student, Department of Horticulture, College of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| 2Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran | ||
| 3Department of Horticulture, College of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| 4Research Institute of Forests and Rangelands, Tehran, Iran | ||
| 5Department of Genetics, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran | ||
| 6Department of Horticulture, College of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| چکیده [English] | ||
| The effect of methyl jasmonate (MeJa) upon Agastache foeniculum [Pursh] Kuntze was investigated in aspects of enzymatic activities of phenylalanine ammonialyase (PAL) and 4-coumarate:CoA ligase (4CL), total phenolic content and total protein. The experiments were carried out based on a completely randomized design under a hydroponic system. The plants were subjected to different concentrations of MeJa (0, 0.1 and 1 mM). The PAL activity significantly increased in plants treated with 1 mM MeJa for 24 h compared with 0.1mM MeJa and control. In addition, 4CL activity also significantly increased at 24 h after 1mM MeJa treatment compared with 0.1mM MeJa treatment and control. However, MeJa treatments did not have any significant effect on total phenolic compounds after 8, 12 or 24 h of treatment compared with controls of those times. Total protein content significantly increased with 1 or 0.1 mM MeJa treatments after 24h of treatment compared with control plants. | ||
| کلیدواژهها [English] | ||
| methyl jasmonate, Agastache foeniculum [Pursh] Kuntze, phenylalanine ammonialyase, 4-Coumarate CoA ligase, Phenolic compounds | ||
| مراجع | ||
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