asghari, A., shokrpour, M., mohammad doost chaman abad, H., panahi, E., imani, A. (2011). Evaluation of genetic diversity of Dactylis glomerata L. ecotypes using RAPD markers. , 18(2), 214-226.
ali asghari; majid shokrpour; hamidreza mohammad doost chaman abad; elham panahi; aliakbar imani. "Evaluation of genetic diversity of Dactylis glomerata L. ecotypes using RAPD markers". , 18, 2, 2011, 214-226.
asghari, A., shokrpour, M., mohammad doost chaman abad, H., panahi, E., imani, A. (2011). 'Evaluation of genetic diversity of Dactylis glomerata L. ecotypes using RAPD markers', , 18(2), pp. 214-226.
asghari, A., shokrpour, M., mohammad doost chaman abad, H., panahi, E., imani, A. Evaluation of genetic diversity of Dactylis glomerata L. ecotypes using RAPD markers. , 2011; 18(2): 214-226.

Evaluation of genetic diversity of Dactylis glomerata L. ecotypes using RAPD markers

تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران
Article 6, Volume 18, Issue 2, February 2011, Pages 214-226    PDF (241.17 K)
Document Type: Research Paper
Authors
ali asghari* ; majid shokrpour; hamidreza mohammad doost chaman abad; elham panahi; aliakbar imani
Abstract
Determining genetic diversity of plant materials is necessary in the beginning of any breeding program. In this study, genetic diversity within and among 15 orchardgrass ecotypes collected from different parts of Iran, 7 foreign ecotypes (USA, Russia, Stonia, Spain) and  3 ecotypes with unknown origin were evaluated using RAPD markers. Eight primers from 40 tested RAPD primers produced 73 polymorphic bands. The total number of polymorphic bands within ecotypes varied from 25 to 49 bands. The averages of total and within ecotypes genetic diversity and the degree of genetic differentiation were estimated 0.291, 0.181 and 0.378, respectively. Molecular variance analysis based on squared Euclidean distances indicates that larger proportions of variability existed within ecotypes (78.37 %). Cluster analysis of molecular data, grouped the entries under study into 8 cluster. The grouping based on molecular data had not correspondence with the geographical pattern. Using principal coordinate analysis, the first tree coordinates determined 59.48% of the total variation. Grouping of ecotypes using the first two coordinates confirmed the results of cluster analysis. Cluster analysis of morphological data, grouped the entries into 4 cluster, but great match between the results of morphological and molecular data weren’t observed. In this study, a great amount of genetic diversity observed between and within orchardgrass ecotypes that could be used for selection suitable varieties and the ecotypes with exceeding genetic distance could be used in crossing programs for producing heterosis, mapping populations and disperse generations.
Keywords
Cluster analysis. Genetic diversity; molecular markers; Orchardgrass; RAPDs
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