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زهراگینی چهار جدایه از قارچ Beauveria bassiana روی جمعیّت‌های تابستان‌گذران و زمستان‌گذران سن گندم، Eurygaster integriceps | ||
| مهار زیستی در گیاه‌پزشکی | ||
| مقاله 3، دوره 2، شماره 1، خرداد 1393، صفحه 31-41 اصل مقاله (598.68 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2014.100325 | ||
| نویسندگان | ||
| زینب قمری زارع1؛ حسن عسکری2؛ حبیب عباسی پور1؛ عزیز شیخی گرجان2؛ آیت الله سعیدی زاده1 | ||
| 1دانشکده‌ی کشاورزی دانشگاه شاهد، تهران | ||
| 2موسسه تحقیقات گیاه‌پزشکی کشور، تهران | ||
| چکیده | ||
| در این تحقیق زهراگینی چهار جدایه‌ی ایرانی Beauveria bassiana روی نمونه‌های تابستان‌گذران و زمستان‌گذران حشرات کامل سن گندم به‌روش زیست سنجی از طریق اسپری کردن غلظت‌های مختلف و نمونه‌برداری در طول زمان، مورد بررسی قرار گرفت. سن‌های کامل زمستان‌گذران حساسیّت بیشتری را نسبت به‌سن‌های کامل تابستان‌گذران نشان دادند (F= 69.99; df= 31, 1; P-value= 0.0001). مقادیر LC50 برای جدایه‌ی فشند در مرحله‌ی تابستان‌گذران و مرحله‌ی زمستان‌گذران به‌ترتیب 108×1 و 105×2 اسپور بر میلی‌لیتر محاسبه شد. مقدار LC50 برای جدایه‌ی Spt-566 روی سن‌های زمستان‌گذران، 107×3 اسپور در میلی‌لیتر محاسبه شد. زمان لازم برای ایجاد مرگ و میر 50 درصد سن‌ها (LT50) برای جدایه‌ی فشند با 109 اسپور در میلی‌لیتر در دو مرحله‌ی رشدی سن تابستان‌گذران و زمستان‌گذران به‌ترتیب 13 و 7 روز و برای جدایه‌ی Spt-566 در مرحله‌ی زمستان‌گذران 19 روز محاسبه گردید. جدایه‌ی فشند با کمترین زمان لازم جهت مرگ و میر سن‌ها و پایین‌ترین LC50 به‌عنوان جدایه‌ی زهراگین مؤثر روی سن گندم تعیین شد. همچنین افزودن روغن به سوسپانسیون قارچ باعث افزایش شدت بیمارگری قارچ روی دو جمعیت سن گندم در همه‌ی جدایه‌ها شد. | ||
| کلیدواژه‌ها | ||
| زیست‌سنجی؛ Beauveria bassiana؛ سن گندم؛ مکان زمستان‌گذرانی؛ Eurygaster integriceps | ||
| عنوان مقاله [English] | ||
| Virulence of four Beauveria bassiana isolates on aestivating and overwintering populations of Eurygaster integriceps | ||
| نویسندگان [English] | ||
| Zeinab1 Ghamari Zare1؛ Hassan Askary2؛ Habib Abbasipour1؛ Aziz Sheikhi Gorjan2؛ Ayatollah SaeidiZadeh1 | ||
| چکیده [English] | ||
| In this study, virulence of four Beauveria bassiana isolates on aestivating and overwintering populations of Sunn pest (Eurygaster integriceps) adults was investigated. Bioassays were conducted and performed by spraying fungal spore ssuspension on adult sunn pest. Obtained data was analyzed to compare mortality in different treatments and to estimate LC50 and LT50. The results showed that overwintering adults of Sunn pest were more susceptible than the summer population. According to the results, LC50 of Fashand isolate was 1×108 and 2×105 spore/ml on aestivation and overwintering populations, respectively. LC50 of Spt-566 isolate was 3×107 spore/ml on the overwintering population. Estimated LT50 for Fashand isolate (109 spore/ml) on aestivating and overwintering populations were 13 and 7 days, respectively, whereas, estimated LT50 for Spt-566 isolate was 19 days on overwintering population. On the basis of this study, Fashand isolate was more effective than Spt-566 on two physiological stages of Sunn pest adult. The results also showed that adding oils to the suspension of the spores increase virulence of the isolates on all stages of sunn pest. | ||
| کلیدواژه‌ها [English] | ||
| Bioassay, Beauveria bassiana, Eurygaster integriceps, sunn pest, Overwintering site | ||
| مراجع | ||
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