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Association of NPY and PRL genes with reproductive traits in West Azerbaijan native chicken | ||
| علوم و فنون دامپروری | ||
| Article 2, Volume 4, Issue 17, March 2016, Pages 15-24 PDF (743.74 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/aasrj.2016.106671 | ||
| Abstract | ||
| Neuropeptide Y (NPY) and Prolactin (PRL) are two candidate genes with a wide variety of physiological functions in growth and especially in reproduction processes. The current study was designed to investigate the associations of PRL and NPY gene polymorphism with reproductive traits in West Azerbaijan native chicken. Genomic DNAs were extracted from 100 chickens. Genotyping for the PRL and NPY genes were determined by using PCR-RFLP method. The PCR products from PRL and NPY loci were digested with AluI, and DraI restriction endonuclease, respectively. Two alleles were found for both genes, A and a alleles for NPY, with frequencies of 0.25 and 0.75, T and C alleles for PRL, with frequencies of 0.22 and 0.78, respectively. The association analysis between the polymorphism PRL and NPY genes and reproductive traits were carried out. Significant relationship was not found between genotypes with reproductive traits. The results of current study showed that using information of genes related to reproductive traits couldn't be used to improve the performance of native chicken of East Azerbaijan province. | ||
| Keywords | ||
| PRL and NPY genes; Reproductive traits; Polymorphism; PCR-RFLP | ||
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