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مقایسه مقادیر فنل کل در بافت‌های برگ و میوه ژنوتیپ‌های به و بررسی تأثیر آن در مقاومت به بیماری آتشک | ||
| نهال و بذر | ||
| مقاله 4، دوره 32، شماره 3، آذر 1395، صفحه 331-345 اصل مقاله (248.54 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/spij.2016.113062 | ||
| نویسندگان | ||
| الناز قضاتی1؛ حمید عبداللهی* 2؛ سعید پیری1 | ||
| 1دانشگاه آزاد اسلامی، واحد ابهر، دانشکده کشاورزیو منابع طبیعی، ابهر | ||
| 2پژوهشکده میوه‌های معتدله و سردسیری، مؤسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| چکیده | ||
| فنل‌ها از متابولیت‌های ثانویه مؤثر در تحمل به تنش‌های محیطی در گیاهان است و بافت‌های برگ و میوه درخت به منبع مهمی از این گروه ترکیبات محسوب می‌شوند. پژوهش اخیر با هدف بررسی میزان و تغییرات فنل در بافت‌های برگ و میوه ژنوتیپ‌های به منطقه اصفهان و ارتباط آن با تحمل به بیماری آتشک انجام شد.برای این منظور، دوازده ژنوتیپ گزینش شدهKVD4،SHA1 ، KVD3، PH2، ET1، NB3، KVD1، KM1، SVS1، SVS2،NB2 و شاهد رقم اصفهان، مورد ارزیابی قرار گرفتند. عصاره‌های اتانولی از نمونه‌های برگ در بهار و تابستان واز میوه‌های رسیده در ابتدای پاییز تهیه و با استفاده از معرف فولین سیکالتیو میزان فنل کل بر حسب معادل اسید گالیک اندازه‌گیری شد. نتایج بیانگر این بود که ژنوتیپ‌های مورد مطالعه از نظر میزان فنل کل برگ دارای تفاوت معنی‌داری بودند. ژنوتیپ KVD4 و رقم اصفهان با مقادیر 9/14و 7/2 میلی‌گرم اسیدگالیک برگرم بافت تازه برگ به ترتیب بیش‌ترین و کم‌ترین میزان فنل کل را داشتند. برگ‌های ژنوتیپ‌های به، افزایش پیوسته‌ای را در مواد فنلی تا انتهای تابستان نشان دادند.برخلاف نمونه‌های برگی، تفاوت معنی‌داری در میزان فنل کل میوه‌ها در ارقام و ژنوتیپ‌های مورد بررسی مشاهده نشد. ارزیابی تأثیر میزان فنل به عنوان یکی از مهم‌ترین آنتی‌اکسیدان‌های غیر‌آنزیمی روی توسعه بیماری آتشک، بیانگر همبستگی منفی شاخص حساسیت واریته‌ای (I.V.S) با میزان فنل کل در برگ‌های ابتدای تابستان در ژنوتیپ‌های به مورد بررسی بود. | ||
| کلیدواژه‌ها | ||
| Cydonia oblonga Mill؛ اسید گالیک؛ Erwinia amylovora؛ فنل؛ آتشک | ||
| عنوان مقاله [English] | ||
| Comparison of Total Phenolic Contents of the Leaves and Fruits of Quince Genotypes and its Effects on the Fire Blight Resistance | ||
| نویسندگان [English] | ||
| E. Ghozati1؛ H. Abdollahi2؛ S. Piri1 | ||
| 1respectively, Faculty of Agriculture and Natural Resources, Abhar Branch, Islamic Azad University, Abhar, Iran. | ||
| 2Temperate Fruits Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. | ||
| چکیده [English] | ||
| Phenols are important secondary metabolites with important role in tolerance to the environmental stresses, and quince leaves and fruits are significant sources of phenolic compounds. The current research was conducted to study the contents and variations of total phenolic compounds in the leaves and fruits tissues of selected quince genotypes from Isfahan and investigate its eventual role in the fire blight resistance in this species.Twelve quinces genotypes, KVD4, SHA1, KVD3, PH2, ET1, NB3, KVD1, KM1, SVS1, SVS2, NB2 and cv. Isfahan as control were evaluated.The ethanol extracts of the leaves and fruits were prepared during spring and summer and total phenol contents were determined by the folin-ciocalteu method, based on equivalent gallic acid. The results showed differences in phenolic compounds in the leaves, of the genotypes, so that genotype KVD4 and cv. Isfahan, with 17.8 and 2.7 mg gallic acid/g fresh weight of the leaves, had the highest and lowest total phenols, respectively. Also a constant increase was observed in the phenol contents of the leaves up to end of the summer. On the other hand, no significant difference was found between the amounts of total phenols in fruits of genotypes. The evaluation of phenol content as major components of non-enzymatic anti-oxidative defense system, against fire blight invasion showed negative correlation of Isv, with phenolic contents of the quince leaves from early summer. | ||
| کلیدواژه‌ها [English] | ||
| Cydonia oblonga, gallic acid, Erwinia amylovora, phenol, fire blight | ||
| مراجع | ||
|
Abdollahi, H. 2010. Pear, Botany, Cultivars and Rootstocks. Iranian Agricultural Ministry Publications, Tehran, Iran, 210pp. (in Persian).
Abdollahi, H., Alipour, M., Khorramdel Azad, M., Mehrabipour, S., Ghasemi, A., Adli, M., Atashkar, D., and Akbari, M. 2013. Establishment of quince (Cydonia oblonga Mill.) germplasm collection from various regions of Iran. Acta Horticulturae 976: 199-203.
Abdollahi, H., Ghahremani, Z., and Erfani Nia, K. 2015. Role of electron transport chain of chloroplasts in oxidative burst of interaction between Erwinia amylovora and host cells. Photosynthesis Research 124: 231–242.
Alaa, G. M., Koutb, O. M., Sayed, A., and El-D.H. 2010. Use of hematological parameters to assess the efficiency of quince (Cydonia oblonga Miller) leaf extract in alleviation of the effect of ultraviolet-A radiation on African catfish Clarias gariepinus. Journal of Photochemistry and Photobiology 99: 1-8.
Alipour, M., Abdollahi, H., Abdousi, V., Ghasemi, A. A., Adli, M., and Mohamadi, M. 2014. Evaluation of vegetative and reproductive characteristics and distinctness of some quince (Cydonia oblonga Mill.) genotypes from different regions of Iran. Seed and Plant Improvement Journal 30-1: 507-529 (in Persian).
Amirahmadi, Z. 2015. Evaluation of phenolic and flavonoide compounds and antioxidant capacity and their variations in the tissues of quince cultivars and genotypes from Northern region of Iran. M.Sc. Thesis, College of Agricultire, Islamic Azad University, Karaj Branch, Karaj, Iran. 113pp. (in Persian).
Azarabadi, S. 2014. Assessment of resistance and survey on some tolerance structures to fire blight (Erwinia amylovora) in some new pear cultivars and rootstocks from Iran. M.Sc. Thesis, College of Agricultire, Islamic Azad University, Varamin-Pishva Branch, Varamin, Iran. 128pp. (in Persian).
Bakhriddinov, N.B. 1985. Wild relatives of fruit crops in Central Asia and the upper limit of their distribution. Dep: 3408-85. 14pp. (in Russian).
Bakhshi, D., and Arakawa O.2006. Effects of UV-B irradiation on phenolic compound accumulation and antioxidant activity in ‘Jonathan’ apple influenced by bagging, temperature and maturation. Journal of Food, Agriculture and Environment 4: 75-79.
Costa, R. M., Magalhães, A. S., Pereira, J. A., Andrade, P. B., Valentão, P., Carvalho,M., and Silva, B. M. 2009. Evaluation of free radical-scavenging and antihemolytic activities of quince (Cydonia oblonga) leaf: A comparative studywith green tea (Camellia sinensis). Food Chemistry and Toxicology 47: 860-865.
Ghahremani, Z., Alipour, M., Abdollahi, H., Mohammdi, M., Ghasemi, A., and Adli, M. 2013. Selecting effective indices for evaluation of fire blight resistance in quince germplasm in orchard condition. Acta Horticulturae 1056: 247-252.
Hamauzu, Y., Yasui, H., Inno, T., Kume, C., and Omanyuda, M. 2005. Phenolic profile antioxidant property, and anti-influenza viral activity of Chinese quince(Pseudocydonia sinesis Schneid.), quince (Cydonia oblonga Mill.) and apple (Malus domestica Mill.) fruits. Journal of Agricultural and Food Chemistry 53: 928-934.
Hassani, M. 2013. Isolation and evaluation of diversity of some PR genes in host plants of fire blight and their expression in this interaction. M.Sc. Thesis, College of Agriculture, Islamic Azad University of Tehran, Science and Research Branch, Tehran, Iran, 143pp. (in Persian).
Khoramdel Azad, M., Nasiri, J., and Abdollahi, H. 2013. Genetic diversity of selected Iranian quinces using SSRs from apples and pears. Biochemical Genetics 51: 426-442.
Macheix, J. J., Fleuriet A., and Billot J. 1990. Fruit Phenolics. CRC Press, Boca Raton, Florida, USA. 292pp. Oliveira, A. P., Pereira, J. A., Andrade, P. B., Valentão, P., Seabra, R. M., and Silva, B. M. 2007. Phenolic profile of Cydonia oblonga Miller leaves. Journal of Agricultural and Food Chemistry 55: 7926-7930.
Sabeti, H. 1996. Iranian Forests, Trees and Shrubs. Publications of Agricultural and Natural Resources Organization, Tehran, Iran. 810 pp. (in Persian).
Sawdogo, W. R., Meda, A., Lamien, C. E., Kiendrebeogo, M., Guissou, I., and Nacoulma, O. G. 2006. Phenolic content and antioxidant activity if six Acanthaceae from Burkina Faso. Journal of Biological Sciences 6: 249-252.
Shiraishi, T., Yamada, T., Nicholson, R. L., and Kunoh, H., 1995. Phenylalanine ammonia-lyase in barley: Activity enhancement inresponse to Erysiphe graminis f.sp. hordei (race 1) a pathogen, and pisi,a non-pathogen. Physiological and Molecular Plant Pathology 46: 153-162.
Silva, B. M., Andrade, P. B., Ferreres, F., Domingues, A. L., Seabra, R. M., and Ferreira, M. A. 2002. Phenolic profile of quince fruit (Cydonia oblonga Miller) (pulp and peel). Journal of Agricultural and Food Chemistry 50: 4615-4618.
Silva, B. M., Andrade, P. B., Valentão, P., Ferreres, F., Seabra, R. M., and Ferreira, M. A. 2004. Quince (Cydonia oblonga Miller) fruit (pulp, peel, and seed) and jam antioxidant activity. Journal of Agricultural and Food Chemistry 52: 4405- 4712.
Singh, R. P., Murthy, K. N. C., and Jayaprakasha, G. K. 2002. Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitromodels. Journal of Agricultural and Food Chemistry 50: 81-86.
Sokol-letowska, A., Oszmianski, J., and Wojdylo, A. 2007. Antioxidant activity of the phenolic compounds of hawthorn, pine and skullcap. Food Chemistry 103:853-859.
Venisse, J.S., Guller, G., and Brisset, M.N. 2001. Evidence for the involvement of an oxidative stress in the initiation of infection of pear by Erwinia amylovora. Plant Physiology 125: 2164-2172.
Vermerris, W., and Nicholson, R. 2006. Phenolic Compound Biochemistry. Springer Press, Dordrecht, The Netherlands. 276pp.
Viljevac, M., Dugalic, K., Stolfa, I., Dermic, E., Cvjetkovic, B., Sudar, R., Kovacevic, J., Cesar, V., Lepedus, H., and Jurkovic, Z. 2009. Biochemical basis of apple leaf resistance to Erwinia amylovora infection. Food Technology and Biotechnology 47: 281–287.
Wang, X., Jia, W., and Zhao, A. 2006. Anti-influenza agents from plants and traditional Chinese medicine. Phytotherapy Research 20: 335-341.
Yildirim, H. T. 2006. Evaluation of color parameters and antioxidant activities of fruit wines. InternationalJournal of Food Sciences and Nutrition 57: 47-63.
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